Extraction, isolation and MALDI-QTOF MS/MS analysis of β- d -Glucan from the fruiting bodies of Daedalea quercina

Barrientos, Rodell C.; Clerigo, Melody M.; Paano, Anamy Ma C.

doi:10.1016/j.ijbiomac.2016.08.044

Cited by 24 publications

(8 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Fourier Transform-Infrared Attenuated Total Reflectance (FTIR-ATR) spectra of HWE-VVP or UAE-VVP was detected using a Card-Fourier Infrared Spectrometer (Nicolet, Madison, WI, USA) to determine the chemical bonds, chemical nature and functional groups of extracted polysaccharides. The spectrum was recorded from 400 to 4000 cm −1 by accumulation of16 scans using resolution setting of 4 cm −1 and plotted as % transmittance vs. wavenumber [ 50 ].…”

Section: Methodsmentioning

confidence: 99%

Ultrasound-Assisted Extraction of Polysaccharides from Volvariella volvacea: Process Optimization and Structural Characterization

et al. 2018

View full text Add to dashboard Cite

The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs. A maximum polysaccharide yield of 8.28 ± 0.23% was obtained under the optimized conditions of ultrasound power of 175 W, extraction temperature of 57 °C, extraction time of 33 min, and the ratio of liquid to raw material of 25:1, respectively. Compared to the hot-water extraction, the ultrasound-assistance favored the extraction of polysaccharides from V. volvacea for its higher polysaccharide yield and efficiency. Further preliminary polysaccharide structural characterization indicated that ultrasound treatment affected the monosaccharide compositions and ratios, and molecular weight range of polysaccharides extracted from V. volvacea.

show abstract

Section: Methodsmentioning

confidence: 99%

Ultrasound-Assisted Extraction of Polysaccharides from Volvariella volvacea: Process Optimization and Structural Characterization

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Once the samples were dry, the plate was loaded into the instrument, and high voltage turned on for 20 min prior to data acquisition (to stabilize flight-tube voltages). Adding salt to the DHB matrix can help with carbohydrate ionization (by the formation of Na-adducts) [ 70 ]. Addition of 0.5 uL of 0.5M NaCl to the spotted 1 μL sample (prior to complete drying) did improve signal to noise slightly for low-mass ions.…”

Section: Methodsmentioning

confidence: 99%

“…The DPPH scavenging percentage activity was calculated as follows [ 70 ]: Scavenging ability % = [1 − (A s − A b )/A c ] × 100 where A s is the absorbance of EPS sample with DPPH, A b is the absorbance of the blank which is the absorbance of EPS without DPPH, A c is the absorbance of the negative control which is the absorbance of DPPH alone without EPS.…”

Section: Methodsmentioning

confidence: 99%

Isolation, Characterization and Bioactivities of an Extracellular Polysaccharide Produced from Streptomyces sp. MOE6

Elnahas

Amin

Hussein³

et al. 2017

Molecules

View full text Add to dashboard Cite

A Streptomyces strain was isolated from soil and the sequence of 1471 nucleotides of its 16S rDNA showed 99% identity to Streptomyces sp. HV10. This newly isolated Streptomyces strain produced an extracellular polysaccharide (EPS) composed mainly of glucose and mannose in a ratio of 1:4.1, as was characterized by Fourier transform infrared spectroscopy (FTIR), HPLC and 1H-NMR. The antioxidant activities of the partially purified MOE6-EPS were determined by measuring the hydroxyl free radical scavenging activity and the scavenging of 2,2-diphenyl-2-picryl-hydrazyl (DPPH) radicals. In addition, the partially purified MOE6-EPS showed high ferrous ion (Fe2+) chelation activity which is another antioxidant activity. Interestingly, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays that were colorimetric assays for NAD(P)H-dependent cellular oxidoreductases and a proxy of the number of viable cells, showed that the partially purified MOE6-EPS inhibited the proliferation of the human breast cancer cells (MDA-MB-231). The scratch wound assay showed that MOE6-EPS reduced the migration of mouse breast cancer cells (4T1). This study reports the production of EPS from Streptomyces species with promising antioxidant, metal chelating and mammalian cell inhibitory activities.

show abstract

“…MALDI-TOF-MS spectrometry is a convenient tool for the structural analysis of polysaccharide (Barrientos, Clerigo, & Paano, 2016;Ding et al, 2015Ding et al, , 2016, and it has been used to probe the major connectivity of BTp1 in the present study.…”

Section: Maldi-tof-ms Analysis Of Enzymatic Hydrolysatementioning

confidence: 95%

Structural characterization and immunomodulatory activity of a water soluble polysaccharide isolated from Botrychium ternatum

Zhao

Liu

et al. 2017

Carbohydrate Polymers

View full text Add to dashboard Cite

As a folk medicine, Botrychium ternatum has been used for thousands of years in China. In the present work, a water soluble polysaccharide BTp1 was extracted and purified from B. ternatum. Based on the MALDI matrix 3-aminoquinoline-α-cyano-4-hydroxycinnamic acid, the molecular weight of BTp1 was determined to be 11638Da directly. Monosaccharide analysis showed that BTp1 was composed of arabinose (Ara). Combining enzymatic hydrolysis and subsequent MALDI-TOF analysis, a linear backbone of BTp1, consisted of (1→5)-linked α-l-Araf, was inferred quickly. Then according to NMR experiments, the whole structure of BTp1 was established. The repeating unit of BTp1 was deduced as a linear backbone with branches at O-2, O-3 and its neighboring O-2 positions terminated with (1→)-linked α-l-Araf, respectively. The immunomodulatory assay exhibited that BTp1 could significantly enhance the viability and promote the release of NO in RAW 264.7 cells, suggesting that BTp1 could be a potential immunomodulatory agent in pharmacological fields.

show abstract

Extraction, isolation and MALDI-QTOF MS/MS analysis of β- d -Glucan from the fruiting bodies of Daedalea quercina

Cited by 24 publications

References 38 publications

Ultrasound-Assisted Extraction of Polysaccharides from Volvariella volvacea: Process Optimization and Structural Characterization

Ultrasound-Assisted Extraction of Polysaccharides from Volvariella volvacea: Process Optimization and Structural Characterization

Isolation, Characterization and Bioactivities of an Extracellular Polysaccharide Produced from Streptomyces sp. MOE6

Structural characterization and immunomodulatory activity of a water soluble polysaccharide isolated from Botrychium ternatum

Contact Info

Product

Resources

About