Extraction Method for the Isolation of Terpenes from Plant Tissue and Subsequent Determination by Gas Chromatography

Bowman, Jeremy M.; Braxton, M.Samuel; Churchill, Melissa A.; Hellie, Jade D.; Starrett, Sabrina J.; Causby, Gregory Y.; Ellis, Donald J.; Ensley, Steven D.; Maness, Sheila J.; Meyer, Christopher; Sellers, Joshua R.; Hua, Yang; Woosley, Royce S.; Butcher, David J.

doi:10.1006/mchj.1996.1422

Cited by 24 publications

(9 citation statements)

References 10 publications

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“…Our results are in line with the findings of Bowman et al. (1997), who determined a maceration time of 24 hr for the isolation of terpenes from Abies fraseri .…”

Section: Discussionsupporting

confidence: 93%

Effects of solvent—solvent fractionation on the total terpenoid content and in vitro anti‐inflammatory activity of Serevenia buxifolia bark extract

Truong

Pham

et al. 2021

Food Science & Nutrition

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Severinia buxifolia (Rutaceae) is often used as a traditional medical plant. The present study was carried out to estimate the effects of solvents (petroleum ether and hexane: ethyl acetate) used in liquid–liquid extraction to total terpenoid content (TTC) and in vitro anti‐inflammatory activity of the extracts obtained from S. buxifolia bark. The results showed that solvent fractionation increased the TTC compared with crude extracts. The hexane: ethyl acetate bark extract fraction (HEF) had the highest TTC (731.48 µg/ml) in comparison with the petroleum ether bark extract fraction (PEF) (564.81 µg/ml) and the crude extract (CE) (184.26 µg/ml). In addition, one of composition of terpenoid of S. buxifolia, namely ursolic acid, was determined by HPLC method from the crude CE and the fractions PEF and HEF: 2.44 μg/g DW, 3.56 μg/g DW and 5.04 μg/g DW, respectively. The samples had an in vitro anti‐inflammatory activity comparable with that of two reference standards (aspirin and indomethacin). Particularly, the HEF fraction had the highest in vitro anti‐inflammatory activity (i.e., albumin denaturation: IC50 = 147.91 μg/mL, heat‐induced hemolysis: IC50 = 159.91 μg/mL, proteinase inhibition: IC50 = 117.72 μg/mL, and lipoxygenase activity: IC50 = 90.45 μg/mL). Besides, the preliminary experiments of this study were conducted to determine the influences of maceration factors (solvent type, temperature, and time) for S. buxifolia bark extract. The TTC ranged from 453.70 to 842.59 mg linalool/g DW, and the extraction yield from 2.40% to 5.120% in all extracts. Based on TTC and EY, the hexane: acetone mixture is recommended as the optimal solvent to obtain the crude bark extract (CE) at 46°C for 24 hr of maceration. Extracts of S. buxifolia bark are a promising source for the treatment of inflammatory diseases.

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“…Our results are in line with the findings of Bowman et al. (1997), who determined a maceration time of 24 hr for the isolation of terpenes from Abies fraseri .…”

Section: Discussionsupporting

confidence: 93%

Effects of solvent—solvent fractionation on the total terpenoid content and in vitro anti‐inflammatory activity of Serevenia buxifolia bark extract

Truong

Pham

et al. 2021

Food Science & Nutrition

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“…In 1997 a novel extraction method was proposed, macerating the tissues in the powerful lipophilic solvent dichloromethane (Bowman et al, 1997). These authors confirmed that the molecules identified by gas chromatography and mass spectrometry were identical to those previously noted in the essential oil obtained by hydrodistillation from the needles ( Table 9).…”

Section: Current Knowledge On Abies Extractives and Their Biological supporting

confidence: 52%

Non-wood Forest Products Based on Extractives- A New Opportunity for Canadian Forest Industry Part 2- Softwood Forest Species

Royer¹,

Houde²,

Stevanović³

2013

JFR

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Forest resources are among the most important of Canada (in the case of Quebec, nearly 90% of the territory). Innovation represents an essential challenge for the Canadian forest industry, which is presently undergoing major changes towards finding new solutions for recovery. The processing of forest biomass has become increasingly relevant along with the popular concept of biorefineries. This concept should include the development of novel technologies based on forest extractives. Bioactive molecules are readily available through eco-friendly extraction processes using various types of forest residues including barks which are generated in significant quantities by the industry. This literature review offers a glimpse into the softwood boreal forest with a particular focus on industrial species. We are adopting an ethno-pharmacological approach prior to presenting existing data on bioactive molecules from various sources, along with results from our own laboratory. In conclusion, this paper clearly demonstrates the need for further research on bioactive molecules from Canadian forest species since there remains an important lack of reliable data.

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“…In this study, the isolation method of the extraction with hexane was used. Gas-chromatography (GC) and/or the combination of gas-chromatography and mass-spectroscopy (GC-MS) are commonly used for terpene determination [ 23 , 24 , 25 , 26 , 27 , 28 , 29 ]. The method of GC-MS was used in this study.…”

Section: Introductionmentioning

confidence: 99%

Release of Terpenes from Fir Wood during Its Long-Term Use and in Thermal Treatment

Kačík

Veľková

Šmíra³

et al. 2012

Molecules

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Building structures made from fir wood are often attacked by wood-destroying insects for which the terpenes it contains serve as attractants. One of the possibilities for extending the lifetime of structures is to use older wood with a lower content of terpenes and/or thermally modified wood. The study evaluated the levels of terpenes in naturally aged fir wood (108, 146, 279, 287 and 390 years) and their decrease by thermal treatment (the temperature of 60 °C and 120 °C, treatment duration of 10 h). Terpenes were extracted from wood samples by hexane and analyzed by gas-chromatography mass-spectrometry (GC-MS). The results indicate that recent fir wood contained approximately 60 times more terpenes than the oldest wood (186:3.1 mg/kg). The thermal wood treatment speeded up the release of terpenes. The temperature of 60 °C caused a loss in terpenes in the recent fir wood by 62%, the temperature of 120 °C even by >99%. After the treatment at the temperature of 60 °C the recent fir wood had approximately the same quantity of terpenes as non-thermally treated 108 year old wood, i.e., approximately 60–70 mg/kg. After the thermal treatment at the temperature of 120 °C the quantity of terpenes dropped in the recent as well as the old fir wood to minimum quantities (0.7–1.1 mg/kg). The thermal treatment can thus be used as a suitable method for the protection of fir wood from wood-destroying insects.

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Extraction Method for the Isolation of Terpenes from Plant Tissue and Subsequent Determination by Gas Chromatography

Cited by 24 publications

References 10 publications

Effects of solvent—solvent fractionation on the total terpenoid content and in vitro anti‐inflammatory activity of Serevenia buxifolia bark extract

Effects of solvent—solvent fractionation on the total terpenoid content and in vitro anti‐inflammatory activity of Serevenia buxifolia bark extract

Non-wood Forest Products Based on Extractives- A New Opportunity for Canadian Forest Industry Part 2- Softwood Forest Species

Release of Terpenes from Fir Wood during Its Long-Term Use and in Thermal Treatment

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