Extraction methods, chemical characterizations and biological activities of mushroom polysaccharides: A mini-review

Gong, Pin; Wang, Siyuan; Li, Meng; Chen, Fuxin; Yang, Wenjuan; Chang, Xiangna; Liu, Ning; Zhao, Yuanyuan; Wang, Jing; Chen, Xuefeng

doi:10.1016/j.carres.2020.108037

Cited by 117 publications

(64 citation statements)

References 81 publications

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“…Polysaccharide is defined as a natural macromolecule compound consisting of 10 or more monosaccharides linked by glycosidic bonds and commonly found in plants, animals and microorganisms [1] . A growing number of researches have shown that edible fungus polysaccharides are often used as nutrients to regulate immune activity and have massive biological activities, such as antitumor, antioxidant, anti‐inflammatory, hypoglycemic, and hepatoprotective effects [1–4] . And researchers also found that the biological activities of polysaccharides are closely linked to its chemical structures [5–7] …”

Section: Introductionmentioning

confidence: 99%

The Structural Characterization of a Novel Water‐Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor‐Bearing Mice

Niu

Liu

et al. 2021

Chemistry & Biodiversity

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In the present study, a novel cold water‐soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated. The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853. Its backbone was composed of α‐D‐Glu, α‐D‐Xyl, α‐D‐Gal and α‐L‐Fuc. The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose‐dependent manner. By analyzing Annexin V‐FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria‐mediated apoptotic pathway. Our results could provide valuable information for the potential application of LGP as an anti‐hepatoma agent.

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Section: Introductionmentioning

confidence: 99%

The Structural Characterization of a Novel Water‐Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor‐Bearing Mice

Niu

Liu

et al. 2021

Chemistry & Biodiversity

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“…S2 possesses the broadest antiviral spectrum, suggesting that the antiviral bioactive compounds may have a higher solubility in water than in organic solvent. Hot water extraction is regarded as a common and simple method to extract water-soluble polysaccharides from Ganoderma mushrooms (Ling-Sing Seow et al, 2013;Nie et al, 2013;Ferreira et al, 2015;Zhu et al, 2015;Gong et al, 2020). In addition, the antiviral bioactive compounds present in S2 appear to be thermally stable and were not negatively affected during the heating process as demonstrated by the results.…”

Section: Discussionmentioning

confidence: 88%

In vitro antiviral activity of medicinal mushroom Ganoderma neo-japonicum Imazeki against enteroviruses that caused hand, foot and mouth disease

et al. 2021

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Hand, foot and mouth disease (HFMD) is a common pediatric infectious disease caused by Enterovirus A species (EV-A) within the family of Picornaviridae. Enteroviruses are nonenveloped and positive-sense single-stranded RNA viruses (Linden et al., 2015). Enterovirus A71 (EV-A71) and coxsackievirus A16 (CV-A16) are the most prevalent etiological pathogens for HFMD (Klein & Chong, 2015). However, CV-A10 and CV-A6 are rapidly emerging and being increasingly reported to cause HFMD outbreaks worldwide such as in

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“…In the case of free radical scavenging, polysaccharides are believed to have greater potential than monosaccharides. This activity may be determined by the size of molecules and the type of binding in the side branches of the main chain of the polysaccharides; nevertheless, their antioxidative activity is still rather moderate [36]. A previous study reported the antioxidant activity of polysaccharides from S. crispa revealed in the ABTS •+ assay.…”

Section: Biological Activity Of Cpsmentioning

confidence: 99%

“…The fruiting body of S. crispa contains approximately 13.4 g of protein, 21.5 g of carbohydrates, and 2.0 g of fat per 100 g of dry weight [19]. Ultrasonic-assisted extrac-tion (with water as an eluent) was used to obtain polysaccharides, since the ultrasonic enhancement of the process causes the disruption of cell walls, reduces particle size, and enhances transfer of cell components resulting in better extraction efficiency than traditional techniques [36]. The isolation of the crude polysaccharides was preceded by preliminary extraction with ethanol (to remove small molecules) and involved the deproteinization method (with Sevage reagent).…”

Section: Chemical Composition Of Cpsmentioning

confidence: 99%

Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study

et al. 2021

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The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from S. crispa (CPS) in one of the most common cancer types—colon cancer. The determination of the chemical composition of CPS has revealed that it contains mostly carbohydrates, while proteins or phenolics are present only in trace amounts. 1H NMR and GC–MS methods were used for the structural analysis of CPS. Biological activity including anticancer, anti-inflammatory and antioxidant properties of CPS was investigated. CPS was found to be non-toxic to normal human colon epithelial CCD841 CoN cells. Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29. Antioxidant activity was determined by various methods and revealed the moderate potential of CPS. The enzymatic assays revealed no influence of CPS on xanthine oxidase and the inhibition of catalase activity. Moreover, pro-inflammatory enzymes such as cyclooxygenase-2 or lipooxygenase were inhibited by CPS. Therefore, it may be suggested that S. crispa is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.

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Extraction methods, chemical characterizations and biological activities of mushroom polysaccharides: A mini-review

Cited by 117 publications

References 81 publications

The Structural Characterization of a Novel Water‐Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor‐Bearing Mice

The Structural Characterization of a Novel Water‐Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor‐Bearing Mice

In vitro antiviral activity of medicinal mushroom Ganoderma neo-japonicum Imazeki against enteroviruses that caused hand, foot and mouth disease

Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study

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