Extraction of a soluble polysaccharide from Auricularia polytricha and evaluation of its anti-hypercholesterolemic effect in rats

Zhao, Shuang; Rong, Chengbo; Liu, Yu; Xu, Feng; Wang, Shouxian; Duan, Chang-Ling; Chen, Jie; Wu, Xuezhong

doi:10.1016/j.carbpol.2014.12.041

Cited by 69 publications

(43 citation statements)

References 33 publications

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“…In the anomeric region, the signals at 110.24 ppm were assigned to alpha-Araf, the signals at 105.1 and 98.35 ppm were assigned to beta-Glcp and alpha-Glcp, the signals at 101.27 and 101.24 ppm were assigned to beta-Galp [29], and the signals at 100.51 and 100.31 ppm were assigned to alpha-Manp. The signals for carbons of alpha-Araf and beta-Glc (1→6) were obviously stronger than those of beta-Galp and Glc [30]; this suggested that Ara and beta-Glc (1→6) residues were present in flexible side chains. The chemical shift at 19.4 ppm was assigned to the methyl group of Rha, which was consistent with the detected sugar compositions.…”

Section: Structural Characterization Of S-3-1mentioning

confidence: 97%

“…Guinea pig serum was diluted with BBS to a concentration giving about 50% hemolysis. Triplicate samples containing 50 L of each serially diluted polysaccharide fraction were mixed with 50 L diluted serum, added to microplate wells, and incubated at 37 ∘ C. After 10 min, sensitized sheep erythrocytes (100 L) and BBS (100 L) were added to each well, and the samples were incubated for an additional 30 …”

Section: Complement-fixing Assaymentioning

confidence: 99%

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Preliminary Characterization of a Homogeneous Polysaccharide with Anticomplement Activity from Sijunzi Decoction

Wang

Peng

et al. 2018

Journal of Chemistry

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Sijunzi decoction (SJZD) is a classical herbal prescription in traditional Chinese medicine (TCM) used for enhancing the function of immune system. In previous studies, a polysaccharide fraction S-3 was screened from SJZD by assessment of immune system regulation, intestinal microbiota, and SCFA in order to explore the immune active ingredients in SJZD. In the present study, S-3 was further purified, and a homogeneous polysaccharide S-3-1 with a molecular mass of 13.5 × 10 4 Da was obtained after further fractionation by Sephadex G-150 size-exclusion chromatography. The immunological activities of S-3-1 were assayed in vitro for the first time. The determination of the anticomplement activity showed that S-3-1 displayed inhibitory effects on classical pathway of the complement system, with CH 50 values of 530 g/mL. The FT-IR analysis showed that S-3-1 had absorptive peaks characteristic of polysaccharides. The methylation and GC-MS analysis showed that it is comprised of Rha, Ara, Xyl, Man, Gal, and Glc in a relative molar ratio of 0.35 : 0.37 : 1.4 : 0.31 : 3 : 0.8 and that it mainly contained 1,4-linked-Glc and 1,6-linked-Gal glycosidic bonds. The morphology of S-3-1 was observed by atomic force microscope (AFM). These results provided evidences for tracking the material basis of SJZD immune activity.

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Section: Structural Characterization Of S-3-1mentioning

confidence: 97%

Section: Complement-fixing Assaymentioning

confidence: 99%

Preliminary Characterization of a Homogeneous Polysaccharide with Anticomplement Activity from Sijunzi Decoction

Wang

Peng

et al. 2018

Journal of Chemistry

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“…Therefore, an important prerequisite for expanding the application range of polysaccharides as a food additive hydrocolloid is to reveal the rheological properties of polysaccharides. APPS had anticancer activities (Song and Du, 2010), antitumor effects (Song and Du, 2012), antioxidant (Chen and Xue, 2018) and antihypercholesterolemic effect (Zhao et al, 2015). However, no rheological properties of APPS have been reported in the literature.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Ultrasonic/Microwave Assisted Extraction (UMAE) and Rheological Properties of Polysaccharides from Auricularia polytricha

Zhang¹,

Ding²,

Xu³

et al. 2020

American Journal of Biochemistry and Biotechnology

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Polysaccharides from Auricularia polytricha (APPS) were extracted using Ultrasonic/Microwave Assisted Extraction (UMAE) technology. UMAE conditions of APPS were optimized with Response Surface Methodology (RSM). Rheological properties of APPS were investigated. The results suggested that the optimal UMAE conditions for APPS were ratio of water to raw material 22 (mL/g), extraction time 15.3 min and microwave power 59W. APPS solution exhibited Newtonian flow behavior at low concentration, formed entanglement network at lower concentration and established weak gel structures with solid-like properties at higher concentration. The viscosity of APPS solutions decreased when temperature increased. The viscosity, storage (G') and loss (G") modulus of APPS solution increased when sucrose concentration increased. Heating time and freeze-thaw changes had no significant effect on rheological properties of APPS. The results suggested that UMAE was a suitable and efficient method for APPS extraction and APPS had potential as a novel food additive hydrocolloid in food industry.

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“…Plant-derived polysaccharides are widely used because of their manifold biological properties. For instance, several studies have shown their anti-fatigue activities [9], anti-hypercholesterolemic effects [10], hepato-nephroprotective activities [4], anticoagulant effects [11] or antibacterial properties [12]. Recent data have also demonstrated that these natural biomolecules possess a considerable potential to prevent various oxidative stress-related disorders [13].…”

Section: Introductionmentioning

confidence: 99%

Novel Antioxidant, Anti-α-Amylase, Anti-Inflammatory and Antinociceptive Water-Soluble Polysaccharides from the Aerial Part of Nitraria retusa

Rjeibi

Hentati

Feriani

et al. 2019

Foods

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In this paper, water-soluble polysaccharides (named as NRLP) were extracted from Nitraria retusa leaves. The main structural features of NRLP were determined by High-pressure size exclusion chromatography, Fourier transform infrared and Gas Chromatography/Mass Spectrometry-Electronic Impact analysis. The in vitro and in vivo biological potential of NRLP were evaluated by measuring its antioxidant (•OH and DPPH• scavenging, total antioxidant capacity), anti-α-amylase as well as anti-inflammatory and antinociceptive activities in a mice model. NRLP was composed of Rha (33.7%), Gal (18.1%), GalA (15.0%), Glc (13.3%), Ara (13.3%), Xyl (3.8%), and GlcA (2.8%) and showed a Molecular Weight (Mw) of 23.0 kDa and a polydispersity index (PDI) of 1.66. The investigations highlighted a significant antioxidant activity (IC50 = 2.4–2.6 mg/mL) and an inhibition activity against α-amylase (IC50 = 4.55 mg/mL) in a dose-dependent manner. Further, NRLP revealed interesting anti-edematous effects and antinociceptive activities (both > 70%). These results open up new pharmacological prospects for the water-soluble polysaccharides extracted from Nitraria retusa leaves.

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Extraction of a soluble polysaccharide from Auricularia polytricha and evaluation of its anti-hypercholesterolemic effect in rats

Cited by 69 publications

References 33 publications

Preliminary Characterization of a Homogeneous Polysaccharide with Anticomplement Activity from Sijunzi Decoction

Preliminary Characterization of a Homogeneous Polysaccharide with Anticomplement Activity from Sijunzi Decoction

Optimization of Ultrasonic/Microwave Assisted Extraction (UMAE) and Rheological Properties of Polysaccharides from Auricularia polytricha

Novel Antioxidant, Anti-α-Amylase, Anti-Inflammatory and Antinociceptive Water-Soluble Polysaccharides from the Aerial Part of Nitraria retusa

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