Novel Antioxidant, Anti-α-Amylase, Anti-Inflammatory and Antinociceptive Water-Soluble Polysaccharides from the Aerial Part of Nitraria retusa

Abstract

In this paper, water-soluble polysaccharides (named as NRLP) were extracted from Nitraria retusa leaves. The main structural features of NRLP were determined by High-pressure size exclusion chromatography, Fourier transform infrared and Gas Chromatography/Mass Spectrometry-Electronic Impact analysis. The in vitro and in vivo biological potential of NRLP were evaluated by measuring its antioxidant (•OH and DPPH• scavenging, total antioxidant capacity), anti-α-amylase as well as anti-inflammatory and antinociceptive activities in a mice model. NRLP was composed of Rha (33.7%), Gal (18.1%), GalA (15.0%), Glc (13.3%), Ara (13.3%), Xyl (3.8%), and GlcA (2.8%) and showed a Molecular Weight (Mw) of 23.0 kDa and a polydispersity index (PDI) of 1.66. The investigations highlighted a significant antioxidant activity (IC50 = 2.4–2.6 mg/mL) and an inhibition activity against α-amylase (IC50 = 4.55 mg/mL) in a dose-dependent manner. Further, NRLP revealed interesting anti-edematous effects and antinociceptive activities (both > 70%). These results open up new pharmacological prospects for the water-soluble polysaccharides extracted from Nitraria retusa leaves.

Pharmacological Investigations in Traditional Utilization of Alhagi maurorum Medik. in Saharan Algeria: In Vitro Study of Anti-Inflammatory and Antihyperglycemic Activities of Water-Soluble Polysaccharides Extracted from the Seeds

“…Characteristic bands of polysaccharides were attributed between 527 and 3351 cm −1 . The intense peak at 3351.04 cm −1 corresponded to O-H stretching vibration from polysaccharide and water whereas the signal attributed at 2929.26 cm −1 was assigned to aliphatic bending groups (C-H) [41]. The vibration of carboxylate groups (C=O) were also observed at 1636.30 cm −1 [42].…”

Optimization of complex enzyme‐ultrasonic synergistic extraction of water‐soluble polysaccharides from Perilla frutescens seed meal: Purification, characterization and in vitro antioxidant activity

“…The attractive feature of electron‐transfer classification is that the color of the oxidant changes upon reduction using an antioxidant (Getachew & Chun, 2017). Previous studies have suggested that the antioxidant activity of the polysaccharides can be attributed to their hydrogen‐donating abilities (Rjeibi et al., 2019). The half‐maximal inhibitory concentration (IC 50 ) is an important indicator for evaluating oxidation resistance.…”

Anti-inflammatory activity of a water-soluble polysaccharide from the roots of purple sweet potato

“…In a previous study, a water-soluble polysaccharide isolated from Sedum dendroideum exhibited anti-inammation activity through reducing IL-1b and TNF-a production. 39 Another study also found that the alkali-soluble polysaccharide from purple sweet potato signicantly restrained pro-inammatory agents while enhancing anti-inammatory mediators. 40 These results indicated that WPSPP-1 had potential in vivo anti-inammatory activity.…”