Extraction of atropine by ultrasounds in different solvent systems

Djılanı, Abdelouaheb; Legseir, Belgacem

doi:10.1016/j.fitote.2004.11.004

Cited by 11 publications

(4 citation statements)

References 14 publications

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“…A number of achiral assays have also been reported for the determination of S-Hyosc in plant extracts and pharmaceuticals [22][23][24][25][26] and in human serum [27,28]. Hyosc has been enantioselectively resolved on the ␣ 1 -acid glycoprotein-CSP [29,30], on the human serum albumine-triazine CSP [31] and the teicoplanin-based Chirobiotic T CSP [32].…”

Section: Introductionmentioning

confidence: 99%

HPLC–atmospheric pressure chemical ionization mass spectrometric method for enantioselective determination of R,S-propranolol and R,S-hyoscyamine in human plasma

Siluk¹,

Mager²,

Gronich³

et al. 2007

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

HPLC–atmospheric pressure chemical ionization mass spectrometric method for enantioselective determination of R,S-propranolol and R,S-hyoscyamine in human plasma

Siluk¹,

Mager²,

Gronich³

et al. 2007

Journal of Chromatography B

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“…The alkaloids of interest were isolated from 2.0 g of a wet sample using a chloroformbased extraction method, as described by Djilani and Legseir (2005) [49,50]. The sample was sonicated for 15 min with 10 mL of extraction solution (1:5:1 of chloroform, methanol, and 25% NH 4 OH) and filtered twice with a paper filter.…”

Section: Preparation Of Extracts For Hplc Analysismentioning

confidence: 99%

Modulation of Tropane Alkaloids’ Biosynthesis and Gene Expression by Methyl Jasmonate in Datura stramonium L.: A Comparative Analysis of Scopolamine, Atropine, and Hyoscyamine Accumulation

Rasi,

Sabokdast,

Naghavi

et al. 2024

Life

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Scopolamine and atropine are two medicinal alkaloids derived from Datura stramonium L. with anticholinergic properties. This study explored how methyl jasmonate (MJ), a plant growth regulator, affects the biosynthesis and accumulation of these alkaloids in different plant tissues. The expression levels of putrescine N-methyltransferase (PMT), tropinone reductase I (TR1), and hyoscyamine 6β-hydroxylase (h6h), three critical enzymes in the biosynthetic pathway, were also analyzed. The results indicated that MJ at 150 µM increased the production of scopolamine and atropine in both leaves and roots, while MJ at 300 µM had an adverse effect. Furthermore, MJ enhanced the expression of PMT, TR1, and h6h genes in the roots, the primary site of alkaloid synthesis, but not in the leaves, the primary site of alkaloid storage. These results imply that MJ can be applied to regulate the biosynthesis and accumulation of scopolamine and atropine in D. stramonium, thereby improving their production efficiency.

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“…For the past few years, ultrasonic extraction has become a popular method of obtaining medicinal plant chemical components. [ 11 12 ] Microflow can generate tangential force to plant powder through ultrasonic vibration and can enable the solvent to permeate cells and accelerate active ingredients into the solvent in the plant, thereby improving the extraction rate. At the same time, the activity of extracted substances is not reduced.…”

Section: Introductionmentioning

confidence: 99%

Extraction of chelerythrine and its effects on pathogenic fungus spore germination

Wei

Zhao

2017

Phcog Mag

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Background:Chemical fungicides are widely used to control crop diseases, but these chemicals have adverse effects. They destroy the ecological environment and even have toxic effects on human beings. In this context, the development of botanical pesticides is relevant. One potential botanical pesticide is chelerythrine, a main alkaloid of Chelidonium majus L., which has high antitumor, fungistasis, and antiphlogosis bioactivity.Objective:This study was designed to present an ultrasonic extraction method for chelerythrine and spore germination experiments to inhibit pathogenic fungi. Fungistasis of chelerythrine is now centralized in basic microbiology experiments, such as observing bacteriostatic rings. This study investigates chelerythrine based on pathogenic fungal spore germination and the influence of germ tube elongation.Materials and Methods:Samples of C. majus L., which were wild used in this experiment, were picked from Harbin experimental forest farm of Northeast Forestry University. An L9 (34) orthogonal experiment was designed to optimize the ultrasonic extraction method. All the plant pathogenic fungus strains used in the experiment were preservation strains of Northeast Forestry University Microbial preservation center. Pathogenic fungi were cultivated by joining chelerythrine with and observed germ tube growth and spore germination.Results:The optimum ultrasonication extraction process for chelerythrine has a liquid/solid ratio of 1:8, 35 min of extraction time, 85% of ultrasonic frequency, and 75% of ethanol concentration. When the concentration of chelerythrine was 1.7 × 10−2 mg/ml, the inhibition rates of Septoria microspora Speg. spores and Curvularia lunata were 96.67% and 84.94%, respectively. Moreover, when the concentration of chelerythrine was 1.7 × 10−6 mg/ml, the inhibition rates of S. microspora spores and C. lunata were 47.64% and 12.05%, respectively.Conclusion:Fungistasis activity reached a high level with 1.7 × 10−6 mg/ml of chelerythrine. Chelerythrine has the characteristics of less dosage and obvious fungistasis and has a good prospect for botanical fungicide development.SUMMARY S. microspora spores in chelerythrine concentration of 1.7 × 10−6 mg/ml have an inhibition rate of 47.64%. For chelerythrine concentrations 1.7 × 10−5, 1.7 × 10−4, and 1.7 × 10−3 mg/ml, the inhibition rate is 70%, 80%, and 90%, respectively. When the concentration of chelerythrine was original 1.7 × 10−2 mg/ml, the inhibition rate was 96.67%. As shown in the diagram, the germinal tubes of S. microspora spores were shorter than 50 µm with 1.7 × 10−6, 1.7 × 10−5, and 1.7 × 10−4 mg/ml concentrations of chelerythrine. However, the germinal tubes of spores without chelerythrine could reach 80 µm. With 1.7 × 10−3 mg/ml liquid concentration, the germination was severely inhibited; the germination under concentrate chelerythrine was limited. The inhibitory effect of chelerythrine was greatest in S. microspora. Abbreviations used: C. majus L.: Chelidonium majus L.; Sphaerulina juglandis: S. juglandis; Septoria micr...

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Extraction of atropine by ultrasounds in different solvent systems

Cited by 11 publications

References 14 publications

HPLC–atmospheric pressure chemical ionization mass spectrometric method for enantioselective determination of R,S-propranolol and R,S-hyoscyamine in human plasma

HPLC–atmospheric pressure chemical ionization mass spectrometric method for enantioselective determination of R,S-propranolol and R,S-hyoscyamine in human plasma

Modulation of Tropane Alkaloids’ Biosynthesis and Gene Expression by Methyl Jasmonate in Datura stramonium L.: A Comparative Analysis of Scopolamine, Atropine, and Hyoscyamine Accumulation

Extraction of chelerythrine and its effects on pathogenic fungus spore germination

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