Extraction of Drug from the Biological Matrix: A Review

Prabu, S.; Suriyaprakash, T.N.K.

doi:10.5772/32455

Cited by 25 publications

(14 citation statements)

References 34 publications

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“…S.Lakshmana Prabu and T.N.K. Suriyaprakash in their review mentioned that LLE is more time consuming however their study is on drug extraction [11,17]. On cost effective front LLE was much more cost-effective than SPE.…”

Section: Resultsmentioning

confidence: 99%

Liquid–Liquid Extraction and Solid Phase Extraction for Urinary Organic Acids: A Comparative Study from a Resource Constraint Setting

et al. 2016

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Pre analytical process of extraction for accurate detection of organic acids is a crucial step in diagnosis of organic acidemias by GCMS analysis. This process is accomplished either by solid phase extraction (SPE) or by liquid-liquid extraction (LLE). Both extraction procedures are used in different metabolic laboratories all over the world. In this study we compared these two extraction procedures in respect of precision, accuracy, percent recovery of metabolites, number of metabolites isolated, time and cost in a resource constraint setup. We observed that the mean recovery from SPE was 84.1 % and by LLE it was 77.4 % (p value \0.05). Moreover, the average number of metabolites isolated by SPE and LLE was 161.8 ± 18.6 and 140.1 ± 20.4 respectively. The processing cost of LLE was economical. In a cost constraint setting using LLE may be the practical option if used for organic acid analysis.

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Section: Resultsmentioning

confidence: 99%

Liquid–Liquid Extraction and Solid Phase Extraction for Urinary Organic Acids: A Comparative Study from a Resource Constraint Setting

et al. 2016

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“…Even though analyte get retained on the cartridge, the interferences can be eluted or washed, that results in the analyte-free from interferences. Then the analyte is eluted with elution solvent and either directly injected in or evaporated to dryness followed by dilution with the HPLC mobile phase [15,16].…”

Section: Solid Phase Extractionmentioning

confidence: 99%

“…In protein precipitation method, the analyte should be freely soluble into the reconstituting solvent. Either by converting soluble protein to a nonsoluble state that salting out or by the addition of water miscible precipitation solvent or organic solvents such as acetone, ethanol, acetonitrile or methanol, this technique can be achieved [10,15].…”

Section: Protein Precipitationmentioning

confidence: 99%

Bioanalytical Method Development and Validation: A Review

Deshpande¹,

Kasture²,

Mohan³

et al. 2019

Recent Advances in Analytical Chemistry

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“…Various methods have been employed for extraction of drug from plasma which include A. Liquid-liquid extraction (LLE), B. Solid phase extraction (SPE) and C. Protein Precipitation method 22 . Protein precipitation is the simple method of extraction as compared to the LLE and SPE.…”

Section: Biological Matrix Employed In Bioanalysismentioning

confidence: 99%

A Validated Bioanalytical Method for Quantification of Ziprasidone in Human Plasma by RP-HPLC: Application to a Pharmacokinetic Study.

Ahmed¹,

Shaikh²,

Ibrahim³

2017

JCPR

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A simple and robust analytical reversed-phase high-performance liquid chromatography method was developed and validated for Ziprasidone (ZPS) with Albendazole as internal standard drug. The method was developed in human plasma and dosage formulation with high-quality chromatographic separation between the drug peaks by using a stainless steel Reverse Phased analytical column C18 (25 cm, 4.6 mm id, 5 mm particle size). The system was operated at 25 o C using a mobile phase consisting of Phosphate buffer: acetonitrile [60:40, v/v] with pH adjusted 2.5 with ortho phosphoric acid in isocratic flow rate of 1 mL min-1 with ultraviolet detection ,UV 730 D monitored at 230 nm. The parametric statistics, i.e., correlation coefficient of 0.999, was assessed for all the drugs having linearity over the tested concentration range (15-500 ng mL-1) in plasma using calibration curve. The accuracy of samples for six replicate measurements at lower limit of quantitation level was within limit. The method was applicable for the quality control of the mentioned drugs in raw material, bulk drug and pharmaceutical formulations as well as in pharmacokinetic studies.

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Extraction of Drug from the Biological Matrix: A Review

Cited by 25 publications

References 34 publications

Liquid–Liquid Extraction and Solid Phase Extraction for Urinary Organic Acids: A Comparative Study from a Resource Constraint Setting

Liquid–Liquid Extraction and Solid Phase Extraction for Urinary Organic Acids: A Comparative Study from a Resource Constraint Setting

Bioanalytical Method Development and Validation: A Review

A Validated Bioanalytical Method for Quantification of Ziprasidone in Human Plasma by RP-HPLC: Application to a Pharmacokinetic Study.

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