Extraction of human genomic DNA from whole blood using a magnetic microsphere method

Abstract

With the rapid development of molecular biology and the life sciences, magnetic extraction is a simple, automatic, and highly efficient method for separating biological molecules, performing immunoassays, and other applications. Human blood is an ideal source of human genomic DNA. Extracting genomic DNA by traditional methods is time‐consuming, and phenol and chloroform are toxic reagents that endanger health. Therefore, it is necessary to find a more convenient and efficient method for obtaining human genomic DNA. In this study, we developed urea–formaldehyde resin magnetic microspheres and magnetic silica microspheres for extraction of human genomic DNA. First, a magnetic microsphere suspension was prepared and used to extract genomic DNA from fresh whole blood, frozen blood, dried blood, and trace blood. Second, DNA content and purity were measured by agarose electrophoresis and ultraviolet spectrophotometry. The human genomic DNA extracted from whole blood was then subjected to polymerase chain reaction analysis to further confirm its quality. The results of this study lay a good foundation for future research and development of a high‐throughput and rapid extraction method for extracting genomic DNA from various types of blood samples.

Comparative study of three magnetic nano-particles (FeSO4, FeSO4/SiO2, FeSO4/SiO2/TiO2) in plasmid DNA extraction

“…The present results indicated the potential of the present MNPs for isolation of genomic DNA from mammalian cells. However, improvement of digestion solution for blood cells may increase DNA yield [27].…”

Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay

“…If the sample treatment method does not lyse the membrane or inactivate the inhibitors, then the genotype cannot be determined accurately. To facilitate the release of DNA from cells, various methods have been applied, such as using expensive special materials or complex solvents ( Gong and Li, 2014 , Saavedra-Matiz et al., 2013 ). Despite their efficacy, the utility of these procedures is questionable.…”

Non-instrumented DNA isolation, amplification and microarray-based hybridization for a rapid on-site detection of devastating Phytophthora kernoviae

“…convenient handling, short processing times and the absence of energy requirements. [30][31][32] In order to prove a prospective field application, the isothermal amplification variant HDA was optimized to substitute PCR approaches, which commonly require cost-intensive and power-dependent thermocyclers. Amplification of P. kernoviae DNA was carried out in a miniaturized HDA-zeolite-heater.…”