Extraction of Polysaccharide from Dendrobium nobile Lindl. by Subcritical Water Extraction

Abstract

Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials. Polysaccharides as one of the main ingredients in Dendrobium plants showed obvious biological activity. Thus, SWE of polysaccharides obtained from Dendrobium nobile Lindl. was investigated in this work. The response surface methodology (RSM) was combined with a Box–Behnken design to evaluate the influence that the three independent variables had on the response. The optimal extraction conditions (determined via RSM) were 129.83 °C extraction temperature, 16.71 min extraction time, and 1.12 MPa extraction pressure. The maximum predicted polysaccharide yield was 20.67%, which corresponded well with the experiential extraction (21.88%). The polysaccharides obtained from either the stirring extraction, refluxing extraction, ultrasound extraction, or SWE methods were compared, and the extraction processes were modeled. The molecular weight, monosaccharide composition, and antioxidative activities of the polysaccharides were analyzed.

Subcritical Water Enhanced with Deep Eutectic Solvent for Extracting Polysaccharides from Lentinus edodes and Their Antioxidant Activities

“…From the results, we can conclude that the LEPD sample has a stronger hydroxyl scavenging activity. The experimental results are similar to the experimental results using an only deep eutectic solvent or subcritical water to extract polysaccharides [ 63 , 64 ].…”

Ultrasonic extraction, structural characterization, and antioxidant activity of oligosaccharides from red yeast rice

“…According to Figure 3a, when the extraction temperature was 40°C and the liquid/material ratio was 20 ml/g, the yield of oligosaccharides increased remarkably from 30 to 90 min. However, the yield stopped rising when the extraction time extended due to the extraction equilibrium (Liu et al., 2019). Hence, 90 min was selected as the center point for the response surface methodology based on the Box–Behnken design.…”

“…The scavenging effect of RYRO1 on DPPH was concentration dependent and reached a maximum inhibitory capacity of 34.29% at a concentration of 1 mg/ml, after which there was no further increase. As a positive control, ascorbic acid reached a strong DPPH scavenging capacity at a very low concentration (0.1 mg/ml) (Liu et al., 2019).…”

Multi-Pharmaceutical Activities of Chinese Herbal Polysaccharides in the Treatment of Pulmonary Fibrosis: Concept and Future Prospects

“…PDO is a neutral heteropolysaccharide isolated from the Chinese medicinal material dendrobium candidum, with composed of (1→4)-linked Man and (1→4)-linked Glc, the molar ratio is 5.9:1 and the Mw is 1.78 × 10 5 Da ( He et al, 2016 ; Yu W. et al, 2018 ). It has the effects of anti-oxidation, anti-tumor, anti-viral, anti-aging, lowering blood sugar and promoting hair growth ( Wang J.-H. et al, 2017 ; Liu et al, 2019b ), and is often valued in the field of dermatology. Dendrobium nobile polysaccharides can improve the antioxidant capacity of rats and reduce liver inflammation, so it has an antagonistic effect on liver fibrosis ( Sibinska et al, 2017 ).…”