Extraction, purification and characterization of water soluble galactomannans from Mimosa pudica seeds

Lavudi, Harikrishna Naik; Seshagirirao, Kottapalli; Goycoolea, Francisco M.

doi:10.24190/issn2564-615x/2017/04.07

Cited by 9 publications

(7 citation statements)

References 37 publications

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“…Also the thermal behavior of the isolated polysaccharides strictly depends on the difference in structural and functional groups. The thermal stability of the isolated polysaccharides can be widely used in pharmaceutical industry as an excipient in granule preparation and in food industry as an effective compound for edible film formation (Lavudi et al, ).…”

Section: Resultsmentioning

confidence: 99%

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Extraction and characterization of water‐soluble polysaccharides from Tamarindus indica and Pithecellobium dulce seeds

Sivakumar

Kavitha

2019

J Food Process Engineering

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Water‐soluble polysaccharides (WSP) were secluded from Tamarindus indica (TWSP) and Pithecellobium dulce (PWSP) seeds by hot water method. The physico‐chemical properties revealed that the polysaccharides might be used as a potential food additive. The micrometric properties of TWSP and PWSP possessed a bulk density of 0.64 and 0.68 g/ml, respectively. The isolated polysaccharides were subjected to Fourier transform infrared spectroscopy and Nuclear magnetic resonance spectroscopy for its compositional analysis and exhibited that both the isolates are heteropolysaccharides. The spectra of both the mucilages were resonating at δ 3.00–δ 5.00 ppm which confirmed the presence of polysaccharides. Thermo gravimetric analysis showed that the isolated polysaccharides were thermally stable. Scanning electron microscopy and X‐ray diffraction depicted the morphological characteristics. The radical scavenging ability and antibacterial activity proved that the polysaccharides can be explored as a functional food ingredient. Furthermore, the prebiotic potential of polysaccharides stimulated the growth of Lactobacillus delbrueckii and Lactobacillus fermentum over inulin. Practical applications The study provides optimum extraction conditions to obtain TWSP and PWSP seeds using response surface methodology. The isolated polysaccharides can be used as therapeutic agent and as a food additive due to its high purity obtained from Fourier transform infrared spectroscopy and Nuclear magnetic resonance spectroscopic analysis. The excellent flowability and compressibility of polysaccharides can be applied in pharmaceutical formulation as an excipient. The active component present in the isolated polysaccharides can be used as a food preservative. The supplement of TWSP and PWSP as a carbon source accelerated the growth of probiotics which could be used as natural prebiotics. Thus the study concluded that the water‐soluble polysaccharide obtained from the agro‐industrial by product has the wide application in food and pharmaceutical industries.

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Section: Resultsmentioning

confidence: 99%

“…Also it helps in sustained drug release by entrapment of drug particles in the pores. (Lavudi, Kottapalli, & Goycoolea, 2017).…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Extraction and characterization of water‐soluble polysaccharides from Tamarindus indica and Pithecellobium dulce seeds

Sivakumar

Kavitha

2019

J Food Process Engineering

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“…For a better comparison, figure 6 shows the FT-IR spectra of the prepared galactomannan/Zn(OH) 2 -ZnO composite and the extracted and purified galactomannan as a reference (the same that was used for preparing the composite material, and whose main characteristics were reported previously [26]). For the reference case, it is observed that the FT-IR spectrum shows the characteristic bands usually reported in the literature for galactomannan polysaccharides [35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52]. In this way, the broad band at around 3360 cm −1 observed in the reference spectrum corresponds to the O-H stretching vibrations of the hydroxymethyl (-CH 2 OH) The FT-IR spectrum of the galactomannan reference also presents two bands at 1739 and 1645 cm −1 .…”

Section: 1c Ft-ir Spectroscopy Characterizationmentioning

confidence: 94%

Characterization of the antibacterial galactomannan/ $$\hbox {Zn(OH)}_{2}$$ Zn(OH) 2 –ZnO composite material prepared in situ from a green process using mesquite seeds as a biopolymer source

et al. 2019

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In this work, the preparation of an antibacterial galactomannan/Zn(OH) 2-ZnO composite material by a green process is proposed and demonstrated. The galactomannan polysaccharide was extracted from the arid-native mesquite (Prosopis spp.) seeds through a hydrothermal technique, and it was used as an organic polymeric matrix for the in situ chemical precipitation of the Zn(OH) 2-ZnO inorganic particles from simple reactions between Zn 2+ and NaOH. The preparation was performed under standard conditions in aqueous solutions. The composite material, which was obtained by centrifugation of the previous preparation, was characterized by X-ray diffraction, transmission and scanning electron microscopy, infrared spectroscopy and thermal analysis. By means of these techniques, it was deduced that the composite material is highly crystalline (about 94%), composed of about 92% inorganic and 8% organic portion, and has a predominant size of 760 nm and a mean size of 800 nm. The antibacterial activity of the composite material was also studied, which resulted better against Escherichia coli (Gram-negative bacterium) than against Staphylococcus aureus (Gram-positive bacterium), which was calculated in a difference of about 30%. This work presents the high potential of galactomannans to form functional composite materials with inorganic particles.

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“…Galactomannans of Adenanthera pavonia (Ceraqueira et al, 2009) and Mimosa pudica (MP) were extracted, purified and characterized as described in our previous paper (Harikrishna et al, 2017). Work carried out in University of Hyderabad, University of Munster and MVRDL ESIC, Hyderabad, during 2015-2021.…”

Section: Methodsmentioning

confidence: 99%

“…In a recent accompanying paper we have documented the extraction, purification and characteristics of galactomannan sourced from Adenanthera pavonia (Ceraqueira et al, 2009). Also, the galactomannan from Mimosa pudica L. and Dichrostachys cineria L has been studied in our laboratories (Harikrishna et al, 2017;2018) and these legume trees are indigenous to Southern India. In the present study we have investigated the potential applications of these new galactomannans as building blocks of emulsifier, nanoparticles with potential use in pharmacy and food industry.…”

Section: Viscous Behaviour and Interactive Nature Of Indigenous Legum...mentioning

confidence: 99%

Viscous Behaviour and Interactive Nature of Indigenous Legume Seed Galactomannas and Biodegradable Nanoparticles for Drug Delivery

Lavudi¹,

Jakinala²,

Nanibabu³

et al. 2023

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Background: Galactomannans are the seed polysaccharides also known as seed gums, mostly isolated from the legume seed endosperm. Galactomannans can easily mix with xanthans by physical association, exhibits synergistic effect and form complex mixtures due to their entangle nature in deferent ratios. Polysaccharides having these properties make them versatile materials which are useful in different biomedical and food applications as additives, thickening, gelling agents, emulsifiers and stiffeners. Biopolymer-based colloidal particles have potential application in drug and gene delivery. Methods: Galactomannans extracted from indigenous legume seed endosperm from Adenanthera pavonna L and Mimosa pudica L. and investigated during 2015-2021 for different aspects i.e. viscous behaviour and interaction properties, complex formation with other polysaccharide like xanthan and preparation and physical characterisation of galactomannan-based nanoparticles obtained by covalent cross-linking with genipin. Result: The intrinsic viscosity of A. pavonna and M. pudica measured also compared with the commercial polysaccharide LBG (Locust Bean gum). Prepared small spherical shaped nanoparticles of diameters ~33 to 67 nm and negative zeta potential of ~-9.2 - 19.0 mV were obtained. We suggest that the nanoparticles form due to the covalent crosslinking effect of genipin on the residual protein fraction of both galactomannans. The biodegradable nanoparticles offer to be a potential new platform for the oral delivery of drug and nutraceuticals.

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Extraction, purification and characterization of water soluble galactomannans from Mimosa pudica seeds

Cited by 9 publications

References 37 publications

Extraction and characterization of water‐soluble polysaccharides from Tamarindus indica and Pithecellobium dulce seeds

Extraction and characterization of water‐soluble polysaccharides from Tamarindus indica and Pithecellobium dulce seeds

Characterization of the antibacterial galactomannan/ $$\hbox {Zn(OH)}_{2}$$ Zn(OH) 2 –ZnO composite material prepared in situ from a green process using mesquite seeds as a biopolymer source

Viscous Behaviour and Interactive Nature of Indigenous Legume Seed Galactomannas and Biodegradable Nanoparticles for Drug Delivery

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