Extraction, Separation Isolations and Phytochemical Investigation of the Insulin Plant ‘Costuspictus’

Abstract

Costuspictus (spiral ginger) commonly known as 'Insulin plant' was introduced from Mexico to India (kerala) very recently. The literature survey reveals that the fresh raw leaves should be consumed for anti-diabetic. Despite the preliminary studies the detailed phytochemical investigation has not been reported so far. Therefore, the rhizome extracts of costus pictus in different organic solvents (acetone, ethyl alcohol, chloroform, n-hexane and water). The five extract yielded by cold maceration procedure with the solvent Acetone, n-Hexane, Ethyl alcohol, Chloroform and Water. The maximum extract yield was obtain in ethyl alcohol cold extract which was preferably used for further to phytochemical studies, even though there was negligible difference in the presence of chemical constituents in the extract of samples. The ethanol extract of rhizome has shown maximum number concentration of secondary metabolites. Further phytochemical testing, The phytochemical Investigation reports revels that Insulin Plant 'Costuspictus' The preliminary phytochemical tests indicated the presence of steroids, alkaloids, glycoids, flavonoids, saponin, tannins, in the extracts of rhizome of C. Pictus. The test which gave a positive results are mentioned in above phytochemical tests. The further work will be carried out for investigation of rhizome ethanol extract by thin layer chromatographic and Column chromatographic analysis, and spectroscopic analysis like GC-MS, 1 HNMR. Based on the above results it is evident that the leaves of C. pictus have ant diabetic effect and must be developing remarkable interest and potential in the new researcher for future studies on diabetes mellitus.