Extracts of M. pulegium (L.) and M. spicata(L.): Effect of Extraction Conditions on Phenolics and Flavonoïds Contents and Their Antioxidant Power

Abstract

The correlation between the contents of polyphenols and flavonoïds and the degree of antioxidant activity of various extracts from M. pulegium (L.) and M. spicata (L.), obtained by different solvents and methods, was investigated. The crude extracts were prepared by mixing areal parts in powder with methanol/water solution. They were subjected later to liquid-liquid extraction via solvents with progressive polarity (chloroform, ethyl acetate and n-butanol) by maceration and soxhlet techniques. The total phenol and flavonoïds contents from crude extracts and their fractions were determined by using Folin-Ciocalteu and AlCl3 assays respectively. The antioxidant activity of extracts was evaluated by DPPH • (1,1-Diphenyl-2-picrylhydrazyl) radical scavenging test. This activity was measured by inhibition concentration 50% (IC50) values. Generally, higher extract yields were obtained by the soxhlet extraction technique; the crude extracts recorded the best yields for M. pulegium by soxhlet (26.37%) and maceration (13%) while for M. spicata, the aqueous extract by soxhlet (34.9%) and crude one by maceration (9.4%) showed the higher yields. The higher phenolic and flavonoïds contents were found in crude extracts by maceration for both mints whereas by soxhlet, the ethyl acetate and/or n-butanol extracts demonstrated the strongest contents. These extracts, rich in flavonoïds, showed a positive correlation since they have exhibited better antioxidant activity compared to ascorbic acid as the antioxidant reference (IC50= 0.051 mg/ml).

Novel Perceptions on Chemical Profile and Biopharmaceutical Properties of Mentha spicata Extracts: Adding Missing Pieces to the Scientific Puzzle

“…These findings are consistent with literature studies showing how polar extracts (methanol, ethanol or water) from M. spicata contain higher levels of phenolics compared to non-polar extracts [40][41][42]. In these studies, the quantification of phenolic compounds was mainly conducted through colorimetric assays.…”

“…Acetone is a non-toxic solvent that can be easily mixed with water in order to achieve the highest polarity. However, although there are several studies on M. spicata alcoholic and hydroalcoholic extracts [40][41][42], investigations on acetone and acetone/water extracts from M. spicata are limited. Therefore, the present paper aims to gain new knowledge about the choice of extraction solvents with M. spicata.…”

Investigation of the Antioxidant and Anti-Inflammatory Capacities of Different Extracts from <i>Cistus ladanifer</i> L. Leaves in the Ait Ammart Region (Northern Morocco)

“…The aqueous extract yield is noticeably higher at 15.81% compared to the ethanolic and ethyl acetate extracts, which stand at 7.59 and 2.57%. In general, the efficiency of extraction increased with increasing polarities of the solvent used; this result is almost consistent with the extraction yield of Mentha spicata L. (Zekri et al 2020). Polar solvents can diffuse inside the plant powder and recover as many metabolites as possible.…”

Optimization of a Green Microwave-Assisted Extraction Method to Obtain Multifunctional Extracts of Mentha sp.

“…Quantitative analysis (mg•g −1 of dry sample) of the main phenolic compounds of MAE extracts from different Mentha species was carried out by HPLC-MS in SIM mode (Table 2). Although several studies can be found in the literature comparing the total phenolic content of extracts (generally obtained by solid-liquid extraction or Soxhlet [46][47][48]) from different Mentha species, studies related to the individual concentration of these compounds determined by HPLC are more limited [19,26,49]. As expected, the composition of the MAE extracts obtained in this work was different, depending on the Mentha species considered.…”