Extraembryonic endoderm stem cell lines from common voles of the genus Microtus

Shevchenko, Alexander I.; Mazurok, Nina A.; Zhelezova, A. I.; Yefremov, Y.-A. R.; Shilov, A. A.; Шевела, А. И.; Belevantseva, A. V.; Vlasov, V. I.; Zakian, Suren M.

doi:10.1134/s1022795408110057

Cited by 8 publications

(9 citation statements)

References 16 publications

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“…Cells were derived as previously described (Nesterova et al 1994) and maintained in Ham's F12 medium or in a mixture of Ham's F12 and D-MEM (1:1, GIBCO/BRL) supplemented with 10% fetal bovine serum, 1 mM L-glutamine (GIBCO/BRL), 50 U/ml penicillin (GIBCO/BRL), and 50 µg/ml streptomycin (GIBCO/BRL) at 37°С in an atmosphere of 5% CO 2 . Vole extraembryonic endoderm (XEN) and TS cells used for X-linked gene methylation analysis were maintained as described (Grigor'eva et al 2009;Shevchenko et al 2008Shevchenko et al , 2009). …”

Section: Cell Culturesmentioning

confidence: 99%

“…Thus, in all the subclones one of the two X-chromosomes demonstrated late replication and the expression of stable Xist transcript, making the subclones a convenient system to assess the X-linked gene expression status in vole somatic tissues. We also determined the X-linked gene expression status in two placentas and an XEN cell subclone (Shevchenko et al 2008) of hybrid females M. rossiaemeridionalis×M. arvalis which exhibit imprinted XCI.…”

Section: Mapping Of X-linked Genes In Common Volesmentioning

confidence: 99%

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Difference between random and imprinted X inactivation in common voles

et al. 2010

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During early development in female mammals, most genes on one of the two X-chromosomes undergo transcriptional silencing. In the extraembryonic lineages of some eutherian species, imprinted X-inactivation of the paternal X-chromosome occurs. In the cells of the embryo proper, the choice of the future inactive X-chromosome is random. We mapped several genes on the X-chromosomes of five common vole species and compared their expression and methylation patterns in somatic and extraembryonic tissues, where random and imprinted X-inactivation occurs, respectively. In extraembryonic tissues, more genes were expressed on the inactive X-chromosome than in somatic tissues. We also found that the methylation status of the X-linked genes was always in accordance with their expression pattern in somatic, but not in extraembryonic tissues. The data provide new evidence that imprinted X-inactivation is less complete and/or stable than the random form and DNA methylation contributes less to its maintenance.

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Section: Cell Culturesmentioning

confidence: 99%

Section: Mapping Of X-linked Genes In Common Volesmentioning

confidence: 99%

Difference between random and imprinted X inactivation in common voles

et al. 2010

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“…XEN cell lines have also been derived using ES cell conditions (feeders + LIF). Moreover, XEN cells have reproducibly been derived not only from mouse embryos [58, 70, 85, 86] but also from voles [87] and rats [87–90]. It is not entirely clear which signaling pathways are required for XEN cell maintenance, but PDGF signaling is required for XEN isolation and/or cell expansion.…”

Section: Isolation Of Three Stem Cell Populations Representative Omentioning

confidence: 99%

Troika of the Mouse Blastocyst: Lineage Segregation and Stem Cells

Artus¹,

Hadjantonakis²

2012

CSCR

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The initial period of mammalian embryonic development is primarily devoted to cell commitment to the pluri-potent lineage, as well as to the formation of extraembryonic tissues essential for embryo survival in utero. This phase of development is also characterized by extensive morphological transitions. Cells within the preimplantation embryo exhibit extraordinary cell plasticity and adaptation in response to experimental manipulation, highlighting the use of a regulative developmental strategy rather than a predetermined one resulting from the non-uniform distribution of maternal information in the cytoplasm. Consequently, early mammalian development represents a useful model to study how the three primary cell lineages; the epiblast, primitive endoderm (also referred to as the hypoblast) and trophoblast, emerge from a totipotent single cell, the zygote. In this review, we will discuss how the isolation and genetic manipulation of murine stem cells representing each of these three lineages has contributed to our understanding of the molecular basis of early developmental events.

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“…These SC were obtained from cultured preimplantation blastocysts of mice [37] and common vole [5]. These SC were obtained from cultured preimplantation blastocysts of mice [37] and common vole [5].…”

Section: Sc Of Extraembryonic Endodermmentioning

confidence: 99%

“…The culture of mouse XEN-cells consists of two mutually transforming cell types [37]. However, vole XEN-cells can be passaged for a long time on gelatin-coated substrate in the absence of conditioned medium [5]. However, XEN-cell isolation from voles, apart from cells looking like spilled beads (Fig.…”

Section: Sc Of Extraembryonic Endodermmentioning

confidence: 99%

Stem Cells Giving Rise to Extraembryonic Tissues

et al. 2011

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The review is devoted to characterization of stem cells involved in the formation of extraembryonic tissues during the early development of mammalian embryos. Here we present our results of characterization of stem cells from the trophoblast and extraembryonic endoderm of voles and comparative analysis of these cells and the corresponding mouse cells and discuss possible signal pathways maintaining these cells in undifferentiated state.

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Extraembryonic endoderm stem cell lines from common voles of the genus Microtus

Cited by 8 publications

References 16 publications

Difference between random and imprinted X inactivation in common voles

Difference between random and imprinted X inactivation in common voles

Troika of the Mouse Blastocyst: Lineage Segregation and Stem Cells

Stem Cells Giving Rise to Extraembryonic Tissues

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