Extrahepatic cholangiography in near-infrared II window with the clinically approved fluorescence agent indocyanine green: a promising imaging technology for intraoperative diagnosis

Wu, Di; Xue, Dingwei; Zhou, Ji; Wang, Yifan; Feng, Zhe; Xu, Junjie; Lin, Hui; Qian, Jun; Cai, Xiujun

doi:10.7150/thno.41127

Cited by 54 publications

(46 citation statements)

References 58 publications

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“…Our previous study has confirmed the extrahepatic cholangiography in NIR-II window outperformed the conventional NIR-I imaging [38] . Herein, we further investigated the capability of ICG for NIR-IIb fluorescence imaging of biliary tract at both macroscopic and mesoscopic level ( Fig.3a-d).…”

Section: Nir-iib Imaging For Biliary Tractsupporting

confidence: 60%

Protein enhanced NIR-IIb emission of indocyanine green for functional bioimaging

He¹,

Zhang³

et al. 2020

Preprint

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Fluorescence imaging performed in the 1500-1700 nm spectral range (labeled as near-infrared IIb, NIR-IIb) promises high imaging contrast and spatial resolution for its little photon scattering effect and minimum auto-fluorescence. Though inorganic and organic probes have been developed for NIR-IIb bioimaging, most are in preclinical stage, hampering further clinical application. Herein, we showed that indocyanine green (ICG), an US Food and Drug Administration (FDA)-approved agent, exhibited remarkable amount of NIR-IIb emission when dissolved into different protein solutions, including human serum albumin, rat bile, and fetal bovine serum. We performed fluorescence imaging in NIR-IIb window to visualize structures of lymph system, extrahepatic biliary tract and cerebrovascular. Results demonstrated that proteins promoted NIR-IIb emission of ICG in vivo and that NIR-IIb imaging with ICG preserved higher signal-to-background ratio (SBR) and spatial resolution compared with the conventional near-infrared II (NIR-II) fluorescence imaging. Our findings confirm that NIR-IIb fluorescence imaging can be successfully performed using the clinically approved agent ICG. Further clinical application in NIR-IIb region would hopefully be carried out with appropriate ICG-protein solutions.

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Section: Nir-iib Imaging For Biliary Tractsupporting

confidence: 60%

Protein enhanced NIR-IIb emission of indocyanine green for functional bioimaging

He¹,

Zhang³

et al. 2020

Preprint

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“…2a). [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] However, the uorescence efficiency (F) decreases remarkably with wavelength (l), especially in low energy bandgap NIR-II materials, as intramolecular motions around the single or double bond result in dominant non-radiative relaxation dynamics (Fig. 2b).…”

Section: Introductionmentioning

confidence: 99%

Structural and process controls of AIEgens for NIR-II theranostics

et al. 2021

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“… 124 Similarly, Wu et al employed ICG to visualize the bile duct injury in the NIR‐II window and obtained better SNR with deep penetration (Figure 5L‐M). 125 Except for commercial fluorescent dyes, Sun et al obtained FD‐1080 J ‐aggregates by self‐assembly of their previously synthesized FD‐1080 (a NIR‐II fluorophore) and 1,2‐dimyristoyl‐sn‐glycero‐3‐phosphocholine (DMPC) via film dispersion, which can be used in brain and hindlimb vascular imaging beyond 1500 nm (Figure 5N‐O). 126 To conclude, we can expect that the redeployment of previously developed fluorescent dyes can enlighten novel properties and further improve NIR‐II fluorescence‐guided surgery.…”

Section: Discussion and Outlookmentioning

confidence: 99%

Exploiting molecular probes to perform near‐infrared fluorescence‐guided surgery

Zhang

2020

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Near-infrared (NIR) fluorescence imaging can provide real-time navigation for surgeons to discriminate boundaries between lesions and healthy tissue, which serves as a promising tool to enhance precise diagnosis and accurate excision during surgery. Molecular probes with NIR fluorescence can visualize diseased tissue in deep penetration with improved signal-to-noise ratio, which considerably encourages the active participation of NIR fluorescence-guided surgery in the operating room. Although a great quantity of fluorescent probes has been employed in clinical trials, the U.S. Food and Drug Administration only approves an extremely narrow number of contrast agents for clinical use so far. Currently, there remain two significant problems to be addressed in surgical resection: accurate identification of diseased tissue and the preservation of adjacent vital structures. Here, molecular probes with NIR fluorescence are systematically reviewed to offer possible solutions to these two problems. Targeting strategies of fluorescent probes are introduced, where the strengths and weaknesses of each strategy are presented. Advances in fluorescent probes for the imaging of vital structures, such as nerve and ureter, are also summarized in this review. It is convinced that NIR fluorescence-guided surgery has the potential to improve current surgical resection and ameliorate the postoperative outcomes of diverse diseases.

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Extrahepatic cholangiography in near-infrared II window with the clinically approved fluorescence agent indocyanine green: a promising imaging technology for intraoperative diagnosis

Cited by 54 publications

References 58 publications

Protein enhanced NIR-IIb emission of indocyanine green for functional bioimaging

Protein enhanced NIR-IIb emission of indocyanine green for functional bioimaging

Structural and process controls of AIEgens for NIR-II theranostics

Exploiting molecular probes to perform near‐infrared fluorescence‐guided surgery

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