Extreme ultraviolet (EUV) surface modification of polytetrafluoroethylene (PTFE) for control of biocompatibility

Abstract

a b s t r a c tExtreme ultraviolet (EUV) surface modification of polytetrafluoroethylene (PTFE) was performed in order to enhance the degree of biocompatibility. Polymer samples were irradiated by different number of EUV shots using a laser-plasma based EUV source in the presence of nitrogen gas. The physical and chemical properties of EUV modified PTFE samples were studied using Atomic Force Microscopy, X-ray photoelectron spectroscopy and water contact angle (WCA) methods. Pronounced wall type micro and nano-structures appeared on the EUV treated polymer surfaces resulting in increased surface roughness and hydrophobicity. Stronger cell adhesion and good cell morphology were observed on EUV modified surfaces by in-vitro cell culture studies performed using L929 fibroblasts.

Modification of Polymer Substrates with Extreme Ultraviolet - Potential Application in Cancer Cell Identification

“…Unlike HCV29, the cells were mostly attached to the substrate in clusters, however single dispersed cells could also be observed. Earlier studies showed that EUV processing of PTFE samples gradually oxidise the polymer surface [13]. PTFE sample irradiated with 50 EUV shots demonstrated best cancer cell adhesion and proliferation results (Fig.…”

“…The EUV processing resulted in formation of regular and pronounced wall type structures and modification of the surface chemical properties as described previously [13]. The HCV29 and T24 average cell number attached to polymer surface over the four day cell culture are summarized in graphical representation in Fig.…”

“…The polymer samples were irradiated with 50 or 300 EUV shots at 10 Hz repetition rate in the presence of EUV-ionized nitrogen gas in the interaction region [12]. The detailed arrangement and working of the source on PTFE and other polymers for application in biomedical engineering are documented before [11,13]. Cells grown on samples for 24, 48, 72, and 96 h and prepared for fluorescent microscopy according to procedures described elsewhere [14].…”

In vitro chemotaxis and tissue remodeling assays quantitatively characterize foreign body reaction

“…To prove the material-dependency of these processes, a standard cell culture glass and two biomaterials, polytetrafluorethylene (PTFE) and titanium, were used. These two biomaterials have a broad range of applications in regenerative medicine: PTFE polymers are used for vascular prosthesis, nerve conduits, or subcutaneous augmentation material (Ahad et al, 2015;Cheesborough et al, 2016), whereas titanium is preferably used in dental and bone replacement (Möller et al, 2012;Souza et al, 2015;Mohammed et al, 2014). In addition to the clinical relevance, the chosen materials exhibit different chemical properties, e.g., distinct water surface interaction from hydrophobic to hydrophilic material characteristics, demonstrated by decreasing water contact angles from 127.1° ±2.8° for PTFE, to 55.0° ±3.3° for titanium, down to 28.8° ±2.7° for glass.…”

Extreme Ultraviolet Surface Modification of Polyethylene Terephthalate (PET) for Surface Structuring and Wettability Control

“…However the conventional surface modification tools alter the bulk material mechanical properties [4]. Recent advancements in the laboratory scale laser produced plasma sources provide opportunities to exploit soft X-ray and extreme ultraviolet regions for applications in biomedical engineering [5][6][7][8][9]. The surface structuring of polymers by EUV photons by ablation mechanism has been demonstrated previously.…”