Extremely low frequency electromagnetic field enhances human keratinocyte cell growth and decreases proinflammatory chemokine production

Vianale, Giovina; Reale, Marcella; Amerio, Paolo; Stefanachi, M.; Luzio, S. Di; Muraro, Raffælla

doi:10.1111/j.1365-2133.2008.08540.x

Cited by 120 publications

(94 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Reale et al, 2006] or HL-60 myeloid leukemia cells [Tokalov and Gutzeit, 2004]. Changes in the gene expression of pro-inflammatory genes were also found in skin cells [Vianale et al, 2008]. In these studies, the experimental setup and exposure conditions differed strongly from our study, but all studies used a 50 Hz signal.…”

Section: Discussioncontrasting

confidence: 43%

Low‐frequency electromagnetic fields do not alter responses of inflammatory genes and proteins in human monocytes and immune cell lines

Bouwens

Kleijn

Ferwerda

et al. 2011

Bioelectromagnetics

View full text Add to dashboard Cite

The effects of low frequency electromagnetic fields (LF EMF) on human health are the subject of on-going research and serious public concern. These fields potentially elicit small effects that have been proposed to have consequences, either positive or negative, for biological systems. To reveal potentially weak but biologically relevant effects, we chose to extensively examine exposure of immune cells to two different signals, namely a complex multiple waveform field, and a 50 Hz sine wave. These immune cells are highly responsive and, in vivo, modulation of cytokine expression responses can result in systemic health effects. Using time course experiments, we determined kinetics of cytokine and other inflammation-related genes in a human monocytic leukemia cell line, THP-1, and primary monocytes and macrophages. Moreover, cytokine protein levels in THP-1 monocytes were determined. Exposure to either of the two signals did not result in a significant effect on gene and protein expression in the studied immune cells. Also, additional experiments using non-immune cells showed no effects of the signals on cytokine gene expression. We therefore conclude that these LF EMF exposure conditions are not expected to significantly modulate innate immune signaling. Bioelectromagnetics ß 2011 Wiley-Liss, Inc.

show abstract

Section: Discussioncontrasting

confidence: 43%

Low‐frequency electromagnetic fields do not alter responses of inflammatory genes and proteins in human monocytes and immune cell lines

Bouwens

Kleijn

Ferwerda

et al. 2011

Bioelectromagnetics

View full text Add to dashboard Cite

show abstract

“…Some of these conflicting data might be due to differences in frequency, intensity, duration and also certain type of cell lines. 17 Generally, despite profound studies, there has not been any accepted molecular mechanism to explain the carcinogenic effects of ELF-EMF. 18 Proteomics is a powerful tool to elucidate underlying mechanism of exposed cells.…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of the Effect of Extremely Low-Frequency Electromagnetic Fields (ELF-EMF) With Different Intensities in SH-SY5Y Neuroblastoma Cell Line

et al. 2017

View full text Add to dashboard Cite

Introduction: During the last 3 decades, human is exposed to extremely low frequency electromagnetic fields (ELF-EMF) emitted by power lines and electronic devices. It is now well accepted that ELF-EMF are able to produce a variety of biological effects, although the molecular mechanism is unclear and controversial. Investigation of different intensities effects of 50 Hz ELF-EMF on cell morphology and protein expression is the aim of this study. Methods: SH-SY5Y human neuroblastoma cell line was exposed to 0.5 and 1 mT 50 Hz (ELF-EMF) for 3 hours. Proteomics techniques were used to determine the effects of these fields on protein expression. Bioinformatic and statistical analysis of proteomes were performed using Progensis SameSpots software. Results: Our results showed that exposure to ELF-EMF changes cell morphology and induces a dose-dependent decrease in the proliferation rate of the cells. The proteomic studies and bioinformatic analysis indicate that exposure to 50 Hz ELF-EMF leads to alteration of cell protein expression in both dose-dependent and intensity dependent manner, but the later is more pronounced. Conclusion: Our data suggests that increased intensity of ELF-EMF may be associated with more alteration in cell protein expression, as well as effect on cell morphology and proliferation.

show abstract

“…Moreover, other in vitro studies suggested that ELF-EMF could change the expression of some proteins, which are involved in control of cell proliferation (8,19) while others demonstrate that exposure to ELF-EMF has no effects on cell proliferation, DNA replication, and regulation (20) result of these studies varied with different or opposite effects. Some of these conflicting data might be due to differences in frequency, intensity, duration, and also certain types of cell lines (21). Generally, despite of profound studies, there have not been any accepted molecular mechanisms that explain carcinogenic effects of ELF-EMF (22).…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Extremely Low-Frequency ElectroMagnetic Field (ELF-EMF) With Different Intensities in Rats Hippocampus

Rezaei‐Tavirani

Hasanzadeh

Seyyedi³

et al. 2018

Arch Neurosci

View full text Add to dashboard Cite

Background: Our previous study determined the alteration in behavioral test-related memory and movement in male rats exposed to extremely low frequency electromagnetic field (ELF-EMF). The molecular mechanism of the effect of ELF-EMF on brain damage of rat has not yet fully understood, thus the current study investigated the effect of ELF-EMFs on hippocampus of male rats by the proteomics approach. The aim of this study was to investigate the effects of 50 Hz ELF-EMF at 0.5 and 1 mT 50 Hz for 2 and 4 weeks on male rats and determined protein expression of the hippocampus. Methods: Effects of 0.5 and 1 mT exposures for 2 and 4 weeks on protein expression was determined via proteomics techniques. Statistical analysis of proteome was performed using the Progensis Same Spots software. Bioinformatics analysis based on network was used for determining the mechanism. Results: Two-DE electrophoresis gels determined the effect of ELF-EMF on rat hippocampus, indicating that protein expression was overall down-regulated by increasing ELF-EMF intensity and time. The detected differentially expressed proteins between the groups comprised of Sptan1, Dpysl2, Tpi1, Lap3, Vdac1, and Tppp. Almost all were cytoskeletal proteins, most of which were increased by intensity and time. These proteins attributed to impairment in short memory of hippocampus after exposure to ELF-EMF. The GO analysis based on network, enriched two important biological process, cytoskeletal neurons and metabolic process. Conclusions: This study determined the effect of ELF-EMF, which led to change in protein expression related to the cytoskeleton that contributes to major processes in brain damage.

show abstract

Extremely low frequency electromagnetic field enhances human keratinocyte cell growth and decreases proinflammatory chemokine production

Abstract: Our results show that ELF-EMF modulates chemokine production and keratinocyte growth through inhibition of the NF-kappaB signalling pathway and thus may inhibit inflammatory processes. ELF-EMF could represent an additional therapeutic approach in the treatment of skin injury.

Cited by 120 publications

References 31 publications

Low‐frequency electromagnetic fields do not alter responses of inflammatory genes and proteins in human monocytes and immune cell lines

Low‐frequency electromagnetic fields do not alter responses of inflammatory genes and proteins in human monocytes and immune cell lines

Proteomic Analysis of the Effect of Extremely Low-Frequency Electromagnetic Fields (ELF-EMF) With Different Intensities in SH-SY5Y Neuroblastoma Cell Line

Proteomic Analysis of Extremely Low-Frequency ElectroMagnetic Field (ELF-EMF) With Different Intensities in Rats Hippocampus

Contact Info

Product

Resources

About