Extrusion-based printing of chitosan scaffolds and their in vitro characterization for cartilage tissue engineering

Sadeghianmaryan, Ali; Naghieh, Saman; Sardroud, Hamed Alizadeh; Yazdanpanah, Zahra; Soltani, Y.; Sernaglia, Jessica; Chen, Daniel

doi:10.1016/j.ijbiomac.2020.08.180

Cited by 100 publications

(46 citation statements)

References 47 publications

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“…For example, a chitosan-alginate ion complex was used in hydrogel preparation for its ability to be formed at neutral pH, allowing proteins or drugs to be incorporated uniformly with minimal denaturation and its ability to promote cell proliferation, i.e., enhance phenotype expression of HTB-94 chondrocytes, providing an upgrade to neat chitosan hydrogels [84] (Figure 5). The printability of chitosan, including ion complexes (e.g., chitosan-alginate), was well documented, and 3D-printed chitosan scaffolds are attractive scaffold materials for CTE [71,73,[91][92][93]. growth factors and cytokines [82].…”

Section: Chitosan Hydrogels For Ctementioning

confidence: 99%

Recent Advancements in 3D Printing of Polysaccharide Hydrogels in Cartilage Tissue Engineering

et al. 2021

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The application of hydrogels coupled with 3-dimensional (3D) printing technologies represents a modern concept in scaffold development in cartilage tissue engineering (CTE). Hydrogels based on natural biomaterials are extensively used for this purpose. This is mainly due to their excellent biocompatibility, inherent bioactivity, and special microstructure that supports tissue regeneration. The use of natural biomaterials, especially polysaccharides and proteins, represents an attractive strategy towards scaffold formation as they mimic the structure of extracellular matrix (ECM) and guide cell growth, proliferation, and phenotype preservation. Polysaccharide-based hydrogels, such as alginate, agarose, chitosan, cellulose, hyaluronan, and dextran, are distinctive scaffold materials with advantageous properties, low cytotoxicity, and tunable functionality. These superior properties can be further complemented with various proteins (e.g., collagen, gelatin, fibroin), forming novel base formulations termed “proteo-saccharides” to improve the scaffold’s physiological signaling and mechanical strength. This review highlights the significance of 3D bioprinted scaffolds of natural-based hydrogels used in CTE. Further, the printability and bioink formation of the proteo-saccharides-based hydrogels have also been discussed, including the possible clinical translation of such materials.

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Section: Chitosan Hydrogels For Ctementioning

confidence: 99%

Recent Advancements in 3D Printing of Polysaccharide Hydrogels in Cartilage Tissue Engineering

et al. 2021

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“…Several kinds of biomaterials, for instance, natural polymers and decellularized extracellular matrices (dECMs), are used for tissue-engineering applications. Gelatin, [138][139][140][141] alginate, [138,141,142] chitosan, [143,144] SF, [145,146] and collagen [138,147] are common natural polymers with high biocompatibility and biodegradability that can be used for cell-scaffold purposes. The similar properties of gelatin and natural ECM provide the right conditions for cell migration and make gelatin an excellent natural polymer for the cell's scaffold.…”

Section: Biomaterialsmentioning

confidence: 99%

“…Its excellent characteristics, such as good mechanical strength, biocompatibility, and biodegradability, as well as nontoxicity, make chitosan a perfect candidate for medical and tissue-engineering applications. [143,152] In the fabrication of artificial skin, a natural polymer with excellent biocompatibility, environmental stability, and nontoxic response must be used. Thus, natural polymers with those characteristics, such as SF, are suitable for skin tissue engineering.…”

Section: Biomaterialsmentioning

confidence: 99%

Recent Advances in Regenerative Tissue Fabrication: Tools, Materials, and Microenvironment in Hierarchical Aspects

Ratri

Brilian

Setiawati

et al. 2021

Advanced NanoBiomed Research

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As part of regenerative medicine, artificial, hierarchical tissue engineering is a favorable approach to satisfy the needs of patients for new tissues and organs to replace those with defects caused by age, disease, or trauma or to correct congenital disabilities. However, the application of tissue engineering faces critical issues, such as the biocompatibility of the fabricated tissues and organs, the scaffolding, the complex biomechanical processes within cells, and the regulation of cell biology. Although fabrication strategies, including the traditional bioprinting, photolithography, and organ‐on‐a‐chip methods, as well as combinations of fabrication processes, face many challenges, they are methods that can be used in hierarchical tissue engineering. The strategic approach to synthetic, hierarchical tissue engineering is to use a combination of several technologies incorporating material science, cell biology, additive manufacturing (AM), on‐a‐chip strategies, and biomechanics. Herein, in a review, the current materials and biofabrication strategies of various artificial hierarchical tissues are discussed based on the level of tissue complexity from nano to macrosize and the adaptive interactions between cells and the scaffolding surrounding the incorporated cells.

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“… 19 Functional groups, such as ~OH and ~NH 2 in the CS structure could enhance drug release through hydrogen bond interactions. 20–22 …”

Section: Introductionmentioning

confidence: 99%

“…19 Functional groups, such as ~OH and ~NH 2 in the CS structure could enhance drug release through hydrogen bond interactions. [20][21][22] Ursolic acid (UA) is a traditional medicinal plant in China that exhibits anti-cancer, anti-inflammatory, anti-virus, antibacterial, anti-diabetes, cardiovascular and anti-oxidative properties. [23][24][25][26] Most studies on UA focus on signal transduction pathways, such as transforming growth factor-β/SMAD signal transduction, mitogen-activated protein kinase and nuclear factor κB.…”

Section: Introductionmentioning

confidence: 99%

Ursolic Acid Loaded-Mesoporous Hydroxylapatite/ Chitosan Therapeutic Scaffolds Regulate Bone Regeneration Ability by Promoting the M2-Type Polarization of Macrophages

Yu¹,

Wang²,

Liu³

et al. 2021

IJN

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Purpose Mesoporous hydroxylapatite (MHAP) might be important for bone regeneration, and ursolic acid (UA) has anti-inflammatory effects. Accordingly, we developed, for the first time, ursolic acid-loaded MHAP-chitosan (MHAP-CS-UA) scaffolds to treat bone defects. Methods In vitro, we synthesize biomaterial scaffolds. By SEM, XRD, EDS and FTIR, we test the performance of the hybrid scaffolds. By drug release, flow cytometry, immunofluorescence, alizarin red staining, and Western blotting, we test the anti-inflammatory and osteo-inductive properties of scaffolds. In vivo, we verify osseointegration ability and bone regeneration. Results The MHAP is a rod-shaped structure with a length of 100~300nm and a diameter of 40~60nm. The critical structure gives the micro-scaffold a property of control release due to the pore sizes of 1.6~4.3 nm in hydroxyapatite and the hydrogen bonding between the scaffolds and UA drugs. The released UA drugs could notably inhibit the polarization of macrophages to pro-inflammatory macrophages (M1 type) and promote the expression of osteogenic-related genes (COL1, ALP and OPG) and osteogenic-related proteins (BMP-2, RUNX2 and COL1). Conclusion The MHAP-CS-UA scaffolds have good anti-inflammatory, osseointegration, osteo-inductivity and bone regeneration. And they will be the novel and promising candidates to cure the bone disease.

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Extrusion-based printing of chitosan scaffolds and their in vitro characterization for cartilage tissue engineering

Cited by 100 publications

References 47 publications

Recent Advancements in 3D Printing of Polysaccharide Hydrogels in Cartilage Tissue Engineering

Recent Advancements in 3D Printing of Polysaccharide Hydrogels in Cartilage Tissue Engineering

Recent Advances in Regenerative Tissue Fabrication: Tools, Materials, and Microenvironment in Hierarchical Aspects

Ursolic Acid Loaded-Mesoporous Hydroxylapatite/ Chitosan Therapeutic Scaffolds Regulate Bone Regeneration Ability by Promoting the M2-Type Polarization of Macrophages

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