Exudation of terpenoids by cotton roots

Hunter, R. E.; Halloin, J. M.; Veech, Joseph A.; Carter, W. W.

doi:10.1007/bf02107174

Cited by 12 publications

(11 citation statements)

References 7 publications

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“…The mechanism of how gossypol reaches these external surfaces, either by external synthesis or internal synthesis and transport, are not well understood. Hunter et al (1978) observed that either disturbing the roots of cotton seedlings or inoculating them with the pathogen Rhizoctonia solani also yielded greater levels of gossypol exudate. In this study, gossypol was detected in culture medium, tissue paper used to blot away excess medium off hairy roots, and along hairy roots, thus suggesting that gossypol was released and deposited along epidermal surfaces of these cultures.…”

Section: Discussionmentioning

confidence: 95%

Effect of elicitors on the production of gossypol and methylated gossypol in cotton hairy roots

Frankfater

Dowd

Triplett

2009

Plant Cell Tiss Organ Cult

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Section: Discussionmentioning

confidence: 95%

Effect of elicitors on the production of gossypol and methylated gossypol in cotton hairy roots

Frankfater

Dowd

Triplett

2009

Plant Cell Tiss Organ Cult

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“…The pigment, localized in lysigenous glands distributed in the epidermal layer of most tissues, confers insect resistance and serves as a deterrent to other herbivores. High levels of gossypol are also localized in the epidermal and cortex tissues of cotton roots (Royce et al 1941;Smith 1961;Mace et al 1974) from which gossypol may be secreted extracellularly (Hunter et al 1978). The medicinal properties of gossypol are widely recognized in China, Africa, and India and in folk medicine practices of the southern USA.…”

Section: Introductionmentioning

confidence: 99%

Induction of hairy root cultures from Gossypium hirsutum and Gossypium barbadense to produce gossypol and related compounds

Triplett

Moss

Bland

et al. 2008

In Vitro Cell.Dev.Biol.-Plant

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Hairy root cultures were induced by inoculating cotyledonary leaves and hypocotyl segments from two cotton species, Gossypium hirsutum and Gossypium barbadense, with Rhizobium rhizogenes 15834. For both species, more hairy roots formed on inoculation sites on cotyledonary leaves than on hypocotyls. The addition of sucrose to basal Murashige-Skoog media increased the frequency of hairy root formation, whereas the addition of naphthalene acetic acid (0.54 μM) did not. After transfer to a liquid culture, hairy root growth was very rapid. After 3 wk in liquid culture, both cotton species produced gossypol, a disesquiterpene secondary metabolite with known anticancer activity, and two related methylated derivatives. Most (60-95%) gossypol produced by cultures was retained within the hairy root tissues, but some was found in the media. The average gossypol level observed among 96 different cultures was 15 mg/g of dry culture mass; however, some cultures produced >40 mg/g of dry culture mass. Variation in gossypol levels was greater for cultures arising from different transformation events than for multiple subclones of a single transformant. The high level of gossypol production attained by most of these cultures suggests that they will be valuable for studying the biochemical and molecular aspects of gossypol biosynthesis, capable of producing large amounts of gossypol and related compounds, and useful for generating modified forms of gossypol (e.g., radio-labeled gossypol) for understanding bioactivity mechanisms.

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“…Gossypol and several other polyphenolic substances are synthesized in cotton cells during environmental stress [2]. High levels of gossypol are also localized in the epidermal and cortex tissues of cotton roots [3][4][5] from which it can be secreted extracelluarly [6]. Gossypol plays a major role in defense system of the plant since it repels or poisons insects like Spodoptera sps.…”

Section: Introductionmentioning

confidence: 99%

Efficient Production of Gossypol from Hairy Root Cultures of Cotton (Gossypium hirsutum L.)

Verma

Trivedi²,

Singh³

et al. 2009

CPB

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A protocol for induction and establishment of Agrobacterium rhizogenes mediated hairy root culture of Gossypium hirsutum was developed through infection with the A4 strain and co-cultivation on hormone-free semi-solid MS medium with B5 vitamins. It resulted in the emergence of hairy roots from the leaf explants, 21 days after infection. The transformation of hairy roots was established by PCR amplification of rol B and rol C genes of the Ri plasmid. All root lines expressed gossypol, although distinct inter-clonal quantitative variations were noticed. Five independent hairy root lines were studied for their growth kinetics as well as gossypol production. The yield potentials of one of them superseded others, as well as the non-transformed, in-vitro grown control roots. The content of gossypol in hairy roots reached a level of 2.43 mg/g DW. It was 4.5 times higher than in vitro and 1.47 times higher than in vivo grown roots of G. hirsutum. Selection of high gossypol producing hairy root lines of G. hirsutum can provide an alternative source for large-scale production of gossypol.

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Exudation of terpenoids by cotton roots

Cited by 12 publications

References 7 publications

Effect of elicitors on the production of gossypol and methylated gossypol in cotton hairy roots

Effect of elicitors on the production of gossypol and methylated gossypol in cotton hairy roots

Induction of hairy root cultures from Gossypium hirsutum and Gossypium barbadense to produce gossypol and related compounds

Efficient Production of Gossypol from Hairy Root Cultures of Cotton (Gossypium hirsutum L.)

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