Fabricating electrospun cellulose nanofibre adsorbents for ion-exchange chromatography

Dods, Stewart; Hardick, Oliver; Stevens, Bob; Bracewell, Daniel G.

doi:10.1016/j.chroma.2014.12.010

Cited by 61 publications

(30 citation statements)

References 32 publications

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“…In addition, these mats showed high feed flow rate and low pressure drop due to their large pore sizes. Earlier work [26] also tested the effects of compression and the number of bed layers for the dynamic binding capacity of regenerated cellulose based IEX electrospun membranes. The highest dynamic binding capacity for lysozyme reached~21 mg/mL for carboxylate adsorbents at a compressive pressure of 1 MPa.…”

Section: Introductionmentioning

confidence: 99%

“…There are several approaches to graft ligands on electrospun membrane substrates. Previous studies [24][25][26][27] conjugated a monomeric acid, base or affinity ligand directly to the functional group on the substrate. However, there has been significant work [8][9][10][11][12][13][14][17][18][19] on surface modification by grafting polymers on the flat sheet membrane substrates using ATRP and UV-initiated polymerizations.…”

Section: Introductionmentioning

confidence: 99%

“…As earlier work [24][25][26][27] focused on grafting monomeric cation exchange ligands, our work of grafting and optimizing polymeric anion exchange ligands on the electrospun membrane substrates is novel even though established surface chemistry has been adopted. Since ATRP requires inert condition and is difficult to implement at a large scale, UV-initiated polymerization on electrospun membranes to form IEX membranes was investigated here.…”

Section: Introductionmentioning

confidence: 99%

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Electrospun Weak Anion-Exchange Fibrous Membranes for Protein Purification

2020

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Membrane based ion-exchange (IEX) and hydrophobic interaction chromatography (HIC) for protein purification is often used to remove impurities and aggregates operated under the flow-through mode. IEX and HIC are also limited by capacity and recovery when operated under bind-and-elute mode for the fractionation of proteins. Electrospun nanofibrous membrane is characterized by its high surface area to volume ratio and high permeability. Here tertiary amine ligands are grafted onto the electrospun polysulfone (PSf) and polyacrylonitrile (PAN) membrane substrates using UV-initiated polymerization. Static and dynamic binding capacities for model protein bovine serum albumin (BSA) were determined under appropriate bind and elute buffer conditions. Static and dynamic binding capacities in the order of~100 mg/mL were obtained for the functionalized electrospun PAN membranes whereas these values reached~200 mg/mL for the functionalized electrospun PSf membranes. Protein recovery of over 96% was obtained for PAN-based membranes. However, it is only 56% for PSf-based membranes. Our work indicates that surface modification of electrospun membranes by grafting polymeric ligands can enhance protein adsorption due to increased surface area-to-volume ratio.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electrospun Weak Anion-Exchange Fibrous Membranes for Protein Purification

2020

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“…for biosensors, self-cleaning textiles, and nutraceutical delivery systems [92]. By derivatization, the CA nonwovens can also be used for example as adsorbents for ion-exchange chromatography [93]. In this case, the electrospun nonwovens were compressed, regenerated to cellulose, and modified with diethylaminoethyl or carboxylate functionalizations.…”

Section: Electrospinningmentioning

confidence: 99%

Cellulose modification and shaping – a review

Jedvert

Heinze

2017

Journal of Polymer Engineering

142

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Abstract This review aims to present cellulose as a versatile resource for the production of a variety of materials, other than pulp and paper. These products include fibers, nonwovens, films, composites, and novel derivatized materials. This article will briefly introduce the structure of cellulose and some common cellulose derivatives, as well as the formation of cellulosic materials in the micro- and nanoscale range. The challenge with dissolution of cellulose will be discussed and both derivatizing and nonderivatizing solvents for cellulose will be described. The focus of the article is the critical discussion of different shaping processes to obtain a variety of cellulose products, from commercially available viscose fibers to advanced and functionalized materials still at the research level.

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“…One of the main advantages of the produced fibres is their high Specific Surface Area (SSA). Hence, those materials are becoming popular in applications like filtration 2 or catalysis 3 , where higher values of SSA are of importance.…”

Section: Introductionmentioning

confidence: 99%

Assessing the Increase in Specific Surface Area for Electrospun Fibrous Network due to Pore Induction

Katsogiannis

Vladisavljević

Georgiadou

et al. 2016

ACS Appl. Mater. Interfaces

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The effect of pore induction on increasing electrospun fibrous network specific surface area was investigated in this study. Theoretical models based on the available surface area of the fibrous network and exclusion of the surface area lost due to fibre-to-fibre contacts, were developed. The models for calculation of the excluded area are based on Hertzian, Derjaguin-Muller-Toporov (DMT) and Johnson-Kendall-Roberts (JKR) contact models. Overall, the theoretical models correlated the network specific surface area to the material properties including density, surface tension, Young's modulus, Poisson's ratio as well as network physical properties such as density and geometrical characteristics including fibre radius, fibre aspect ratio and network thickness. Pore induction proved to increase the network specific surface area up to 52%, compared to the maximum surface area that could be achieved by non-porous fibre network with the same physical properties and geometrical characteristics. The model based on Johnson-KendallRoberts contact model describes accurately the fibre-to-fibre contact area under the experimental conditions used for pore generation. The experimental results and the theoretical model based on Johnson-Kendall-Roberts contact model show that the increase in network surface area due to pore induction can reach to up to 58%.

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Fabricating electrospun cellulose nanofibre adsorbents for ion-exchange chromatography

Abstract: HighlightsOptimised chemistry protocols of DEAE and COO functionalisations.Compression applied after electrospinning improved mechanical strength.Increasing compression and bed layers lowered binding capacities.

Cited by 61 publications

References 32 publications

Electrospun Weak Anion-Exchange Fibrous Membranes for Protein Purification

Electrospun Weak Anion-Exchange Fibrous Membranes for Protein Purification

Cellulose modification and shaping – a review

Assessing the Increase in Specific Surface Area for Electrospun Fibrous Network due to Pore Induction

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