Fabrication and application of an amperometric lysine biosensor based on covalently immobilized lysine oxidase nanoparticles onto Au electrode

Nohwal, Bhawna; Chaudhary, Reeti; Kumar, Parveen; Pundir, C.S.

doi:10.1016/j.ijbiomac.2019.09.213

Cited by 14 publications

(3 citation statements)

References 22 publications

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“…For the preparation of AChENPs/ChONPs, the desolvation method was used by using ethanol [ 34 ]. Firstly, the enzyme (acetylcholinesterase/choline oxidase separately) was dissolved in DW (2 mg/mL).…”

Section: Methodsmentioning

confidence: 99%

An Amperometric Acetylcholine Biosensor Based on Co-Immobilization of Enzyme Nanoparticles onto Nanocomposite

2023

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An electrochemical biosensor was fabricated using nanoparticles of acetylcholinesterase (AChE) and choline oxidase (ChO)/Pt nanoparticles (PtNPs)/porous graphene oxide nanosheet (GONS) composite. A pencil graphite electrode (PGE) was used for the electrodeposition of nanocomposite and the determination of acetylcholine (ACh), a neurotransmitter. Various techniques such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectra and cyclic voltammetry (CV) were used for characterization. This biosensor (AChENPs-ChONPs/GONS/PtNPs/PGE) indicated a very short response time (3 s), a lower limit of detection (0.001 µM), good linearity (0.001–200 µM), longer storage stability (6 months) and better reproducibility. The percent analytical recoveries of added acetylcholine in serum (5.0 and 10 µM) were found to be 97.6 ± 0.7 and 96.5 ± 0.3 for the present biosensor. The coefficients of variation were obtained to be 8% and 3.25%, correspondingly. The biosensor was applied to measure the ACh amount in the serum of healthy individuals and patients with Alzheimer’s disease. The number of interferents had no effect on the biosensor at their physiological concentrations.

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Section: Methodsmentioning

confidence: 99%

An Amperometric Acetylcholine Biosensor Based on Co-Immobilization of Enzyme Nanoparticles onto Nanocomposite

2023

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“…Optimization studies were carried out to measure the lysine content in mozzarella cheese, an important parameter to evaluate the maturity of the cheese. The LOD of this biosensor was higher when compared to an amperometric lysine biosensor using nanoparticles of LyOx from Trichoderma viride [ 93 ], yet it also showed a wider detection range. L-Glutamate is a non-essential amino acid, which occurs naturally in a range of foods including tomatoes, cheese, and mushrooms, and plays an important role in the palatability of food.…”

Section: Enzyme-based Biosensors For Food Analysismentioning

confidence: 99%

Recent Advances in Electrochemical Enzyme-Based Biosensors for Food and Beverage Analysis

Wijayanti,

Tsvik,

Haltrich

2023

Foods

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Food analysis and control are crucial aspects in food research and production in order to ensure quality and safety of food products. Electrochemical biosensors based on enzymes as the bioreceptors are emerging as promising tools for food analysis because of their high selectivity and sensitivity, short analysis time, and high-cost effectiveness in comparison to conventional methods. This review provides the readers with an overview of various electrochemical enzyme-based biosensors in food analysis, focusing on enzymes used for different applications in the analysis of sugars, alcohols, amino acids and amines, and organic acids, as well as mycotoxins and chemical contaminants. In addition, strategies to improve the performance of enzyme-based biosensors that have been reported over the last five years will be discussed. The challenges and future outlooks for the food sector are also presented.

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“…One of the critical steps in the development of an electrochemical sensor is ensuring the transfer of the electrons involved in the reaction, from the sample to a sensor in a highly sensitive, accurate and repeatable manner. To this end, conducting polymer have been widely studied during the last decade along with making them suitable for biosensor applications [33] coupling it to enzymes [34][35][36], antibodies [37][38][39].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical monitoring of alcohol in sweat

Biscay

Findlay

Dennany

2021

Talanta

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Fabrication and application of an amperometric lysine biosensor based on covalently immobilized lysine oxidase nanoparticles onto Au electrode

Cited by 14 publications

References 22 publications

An Amperometric Acetylcholine Biosensor Based on Co-Immobilization of Enzyme Nanoparticles onto Nanocomposite

An Amperometric Acetylcholine Biosensor Based on Co-Immobilization of Enzyme Nanoparticles onto Nanocomposite

Recent Advances in Electrochemical Enzyme-Based Biosensors for Food and Beverage Analysis

Electrochemical monitoring of alcohol in sweat

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