Fabrication and application of zwitterionic phosphorylcholine functionalized monoliths with different hydrophilic crosslinkers in hydrophilic interaction chromatography

Wang, Qiqin; Zhang, Qiaoxuan; Huang, Hongbin; Zhao, Pengxiang; Sun, Lingjue; Peng, Kun; Liu, Xiao; Ruan, Meng; Crommen, Jacques; Yu, Peng; Jiang, Zhengjin

doi:10.1016/j.aca.2019.12.016

Cited by 15 publications

(12 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phosphocholine is a relatively new zwitterion used in the HILIC approach (ZIC-cHILIC), and has the opposite charge arrangement compared to the commercially available ZIC-HILIC (sulfobetaine). Because the positive charge is located on the outermost part of the particle, ZIC-cHILIC has more accessible positive charge to enhance the electrostatic attraction of negatively charged glycopeptides, especially towards negatively charged sialylated glycopeptide [ 33 , 34 ]. Wang et al studied the physicochemical and chromatographic properties of phosphocholine-functionalized monolith columns; a total of 24 glycopeptides, including 19 fucosylated and 2 sialyl-glycopeptide, were enriched from human IgG, suggesting a promising material for N-glycopeptide enrichment [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

“…Because the positive charge is located on the outermost part of the particle, ZIC-cHILIC has more accessible positive charge to enhance the electrostatic attraction of negatively charged glycopeptides, especially towards negatively charged sialylated glycopeptide [ 33 , 34 ]. Wang et al studied the physicochemical and chromatographic properties of phosphocholine-functionalized monolith columns; a total of 24 glycopeptides, including 19 fucosylated and 2 sialyl-glycopeptide, were enriched from human IgG, suggesting a promising material for N-glycopeptide enrichment [ 33 ]. Although a vast number of glycoproteomic workflows have been developed, many suffer from the time-consuming and complicated enrichment process.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

ZIC-cHILIC Functionalized Magnetic Nanoparticle for Rapid and Sensitive Glycopeptide Enrichment from <1 µL Serum

et al. 2021

View full text Add to dashboard Cite

Due to their unique glycan composition and linkage, protein glycosylation plays significant roles in cellular function and is associated with various diseases. For comprehensive characterization of their extreme structural complexity occurring in >50% of human proteins, time-consuming multi-step enrichment of glycopeptides is required. Here we report zwitterionic n-dodecylphosphocholine-functionalized magnetic nanoparticles (ZIC-cHILIC@MNPs) as a highly efficient affinity nanoprobe for large-scale enrichment of glycopeptides. We demonstrate that ZIC-cHILIC@MNPs possess excellent affinity, with 80–91% specificity for glycopeptide enrichment, especially for sialylated glycopeptide (90%) from biofluid specimens. This strategy provides rapidity (~10 min) and high sensitivity (<1 μL serum) for the whole enrichment process in patient serum, likely due to the rapid separation using magnetic nanoparticles, fast reaction, and high performance of the affinity nanoprobe at nanoscale. Using this strategy, we achieved personalized profiles of patients with hepatitis B virus (HBV, n = 3) and hepatocellular carcinoma (HCC, n = 3) at the depth of >3000 glycopeptides, especially for the large-scale identification of under-explored sialylated glycopeptides. The glycoproteomics atlas also revealed the differential pattern of sialylated glycopeptides between HBV and HCC groups. The ZIC-cHILIC@MNPs could be a generic tool for advancing the glycoproteome analysis, and contribute to the screening of glycoprotein biomarkers.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

ZIC-cHILIC Functionalized Magnetic Nanoparticle for Rapid and Sensitive Glycopeptide Enrichment from <1 µL Serum

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The macro‐ and mesopores are present in porous monoliths and are beneficial for rapid mass transfer, high flow rates and low column back pressure [21] . In recent years, organic and inorganic monoliths have been overgrew their use in flow reactors [19,22–24] and chromatography separation [25,26] . Usually composite monoliths of porous materials are in situ prepared by mixing with organic polymers or inorganic silica inside the column [24,25] …”

Section: Introductionmentioning

confidence: 99%

“…[21] In recent years, organic and inorganic monoliths have been overgrew their use in flow reactors [19,[22][23][24] and chromatography separation. [25,26] Usually composite monoliths of porous materials are in situ prepared by mixing with organic polymers or inorganic silica inside the column. [24,25] Metal-organic gels (MOGs) are an attractive catalogue of porous materials which are self-assembled from metal ions and organic ligands through metal-organic coordination, and have found applications in catalysis.…”

Section: Introductionmentioning

confidence: 99%

“…[25,26] Usually composite monoliths of porous materials are in situ prepared by mixing with organic polymers or inorganic silica inside the column. [24,25] Metal-organic gels (MOGs) are an attractive catalogue of porous materials which are self-assembled from metal ions and organic ligands through metal-organic coordination, and have found applications in catalysis. [27][28][29][30][31] As a catalogue of hierarchically porous materials, MOGs provide both meso-/macropores for mass transfer channels and micropores for the immobilization of catalytically active centers.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Supported Metal Nanoparticles in Metal‐Organic Monoliths for Assembly of a Catalytic Microfluidic Reactor

Chen

et al. 2021

ChemNanoMat

View full text Add to dashboard Cite

A novel strategy has been developed to fabricate an Au@CrADC (ADC=5‐aminobenzene‐1,3‐dicarboxylate) metal‐organic monolithic capillary in microfluidic reactors. Monolithic Cr3+‐based metal‐organic gel (MOG) as catalyst support is synthesized inside fused‐silica capillary employing a two‐step procedure including surface modification of carboxyl group to provide anchoring sites and subsequent MOG formation. Subsequently, Au nanoparticles are in situ immobilized in the monolithic MOG support as active catalysts. The resulting Au@CrADC monolithic capillary is used to assemble a catalytic microfluidic reactor. The catalytic activity of the Au@CrADC reactor is probed by using the oxidative alkyne coupling reaction to synthesize 1,3‐diynes. The catalytic microfluidic reactor demonstrates significantly enhanced catalytic activity in comparison with the corresponding reactions under batch conditions. Additionally, the microfluidic reactor shows high stability with no significant catalytic deactivation during repeated uses.

show abstract

In situ synthesis and unidirectional insertion of membrane proteins in liposome-immobilized silica stationary phase for rapid preparation of microaffinity chromatography

Wang

Chen

et al. 2022

Acta Pharmaceutica Sinica B

View full text Add to dashboard Cite

Fabrication and application of zwitterionic phosphorylcholine functionalized monoliths with different hydrophilic crosslinkers in hydrophilic interaction chromatography

Cited by 15 publications

References 29 publications

ZIC-cHILIC Functionalized Magnetic Nanoparticle for Rapid and Sensitive Glycopeptide Enrichment from <1 µL Serum

ZIC-cHILIC Functionalized Magnetic Nanoparticle for Rapid and Sensitive Glycopeptide Enrichment from <1 µL Serum

Supported Metal Nanoparticles in Metal‐Organic Monoliths for Assembly of a Catalytic Microfluidic Reactor

In situ synthesis and unidirectional insertion of membrane proteins in liposome-immobilized silica stationary phase for rapid preparation of microaffinity chromatography

Contact Info

Product

Resources

About