Fabrication and characterization of a collagen coated electrospun poly(3-hydroxybutyric acid)–gelatin nanofibrous scaffold as a soft bio-mimetic material for skin tissue engineering applications

Ramanathan, Giriprasath; Singaravelu, Sivakumar; Raja, M. D.; Nagiah, Naveen; Praveenkumar, Padmapriya; Ruban, K.; Krishnasamy, Kaveri; Natarajan, T. S.; Uma, T.; Perumal, Paramasivan T.

doi:10.1039/c5ra19529b

Cited by 72 publications

(26 citation statements)

References 49 publications

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“…The effective strategy is to load the antibacterial drugs into the wound dressing and release it slowly to the wound to achieve a long-lasting local antibacterial effect. An ideal wound dressing should: 1) Be close to the wound to absorb excess exudate and maintain a moist environment in the wound; 2) be nontoxic and have no immunogenicity; and 3) maintain moisture absorption ability, block the invasion of external bacteria and pathogenic organisms, and be able to release antibacterial drugs slowly to prevent infection of the wound [34][35][36]. In this study, we fabricated porous PHMB/SF sponges with antibacterial function through electrostatic interaction and by freeze-drying.…”

Section: Discussionmentioning

confidence: 99%

Porous Poly(Hexamethylene Biguanide) Hydrochloride Loaded Silk Fibroin Sponges with Antibacterial Function

et al. 2020

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In order to endue silk fibroin (SF) sponges with antibacterial function, positively charged poly(hexamethylene biguanide) hydrochloride (PHMB) was incorporated in SF through electrostatic interaction and by freeze-drying technique. The influence of PHMB on the structure and antibacterial activities of SF sponges was investigated. The zeta potential of SF was increased significantly when PHMB was incorporated in SF. The pores with size from 80 to 300 µm and the microscale holes in the pore walls within PHMB-loaded SF sponges provided the channels of PHMB release. The PHMB loaded in the porous sponges showed continuous and slow release for up to 20 days. Effective growth inhibition of both Escherichia coli and Staphylococcus aureus was achieved when the mass ratio of PHMB/SF was higher than 2/100. These results suggest that the porous PHMB/SF sponges have the potential to be used as a novel wound dressing for open skin wounds.

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Section: Discussionmentioning

confidence: 99%

Porous Poly(Hexamethylene Biguanide) Hydrochloride Loaded Silk Fibroin Sponges with Antibacterial Function

et al. 2020

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“…Green fluorescence is emitted by calcein that has been converted from nonfluorescent calcein through acetoxymethylester hydrolysis by intracellular esterases of viable cells to fluorescent calcein. Blue fluorescence is emitted by DAPI binding to AT regions of sdDNA, therefore marking all present cell nuclei …”

Section: Methodsmentioning

confidence: 99%

Preparation and Characterization of Electrospun Blend Fibrous Polyethylene Oxide:Polycaprolactone Scaffolds to Promote Cartilage Regeneration

et al. 2020

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Fibrous scaffolds can be used to mimic the structure of cartilage extracellular matrix aiming at cartilage regeneration through optimal utilization of such scaffolds and chondrogenic cells. Herein, different types of fibrous structures are manufactured through electrospinning of blends of polyethylene oxide (PEO) and polycaprolactone (PCL), and the physical and chemical characteristics of the produced fiber mats are investigated. The amount of PEO influenced hydrophilicity, biodegradability, and mechanical properties of the blend fibers. To assess the cytotoxicity and biocompatibility of the scaffolds as well as the effect of fiber orientation, in vitro cell culture studies with a chondrogenic cell line (ATDC5) are conducted. The results show no cytotoxicity of the developed fibrous structures. A promising fibrous scaffold technology is presented with potential applications in cartilage tissue engineering.

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“…Cell attachment and proliferation of both NIH 3T3 fibroblast and human keratinocytes (HaCaT) cell lines were quantified for live cell assay at regular time intervals (6, 12, 24 and 48 h), the medium was removed and the cells were fixed with 4% paraformaldehyde and washed with PBS for several times. Furthermore, each cell was stained with calcein AM solution (2 μM; 400 μl) and incubated for 30 min at 37 • C. Then, the plates with scaffold were washed with PBS for several times and viewed at fluorescence microscope (EVOS FLoid Cell Imaging Station, Thermo Fisher Scientific, USA) [26,27].…”

Section: Biological Propertiesmentioning

confidence: 99%

“…About 100 ml [10 5 CFU (colony forming units)] of each bacterial culture was spread over the agar surface (Muller-Hinton agars) using a sterile glass spreader. The plates were then incubated for 24 h at 37 • C. The antibacterial activity was evaluated by measuring the zone of inhibition against the test organism [26].…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Fabrication of chitosan microparticles loaded in chitosan and poly(vinyl alcohol) scaffolds for tissue engineering application

2017

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In recent decades, the use of microparticle-mediated drug delivery is widely applied in the field of biomedical application. Here, we report the new dressing material with ciprofloxacin-loaded chitosan microparticle (CMP) impregnated in chitosan (CH) and poly(vinyl alcohol) (PVA) scaffold for effective delivery of drug in a sustained manner to the wound site. Moreover, the peculiar physiochemical and structural properties of the CH-CMP scaffold has proved better tensile strength and excellent swelling to achieve 82% of drug release. In vitro biocompatibility was done for both scaffold using NIH 3T3 fibroblasts and human keratinocytes (HaCaT) cell lines. In vitro fluorescent activity showed distinct biocompatibility with good cell adhesion and proliferation. However, the CH-CMP scaffold showed best result to act as promising biomaterial in effective drug delivery in tissue engineering.

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Fabrication and characterization of a collagen coated electrospun poly(3-hydroxybutyric acid)–gelatin nanofibrous scaffold as a soft bio-mimetic material for skin tissue engineering applications

Abstract: The collagen coated nanofibrous scaffold mimics the function of the extra cellular matrix with good biocompatibility, cell adhesion, cell proliferation and aids to provide as a promising tool in skin tissue engineering application.

Cited by 72 publications

References 49 publications

Porous Poly(Hexamethylene Biguanide) Hydrochloride Loaded Silk Fibroin Sponges with Antibacterial Function

Porous Poly(Hexamethylene Biguanide) Hydrochloride Loaded Silk Fibroin Sponges with Antibacterial Function

Preparation and Characterization of Electrospun Blend Fibrous Polyethylene Oxide:Polycaprolactone Scaffolds to Promote Cartilage Regeneration

Fabrication of chitosan microparticles loaded in chitosan and poly(vinyl alcohol) scaffolds for tissue engineering application

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