This study investigates the effects of fabric properties and ultrasonic welding on the performance of surgical gowns. For this purpose, eight spunlace fabrics with different structural properties were provided. First, the fabrics’ thickness, tensile strength, elongation at break, air permeability, drape behavior, and surface friction properties were investigated. Then the fabrics were sewn with the ultrasonic sewing machine. Afterward, the sewn fabrics’ seam strength, air permeability and drape behavior were tested. The results were statistically evaluated. Based on the data obtained, a detailed comparison was made between the fabrics with respect to the expectations of the surgical gowns. The higher the polyester content in the fabric, the higher the fabric strength, seam strength and air permeability. However, viscose-rich fabrics have a softer hand and are easier to drape compared to polyester fabrics. Moreover, sewing process leads to a decrease in the drape and air permeability of the fabrics.