Fabrication and Characterization of an OTFT-Based Biosensor Using a Biotinylated F8T2 Polymer

Abstract

Solution‐processable organic semiconductors have been investigated not only for flexible and large‐area electronics but also in the field of biotechnology. In this paper, we report the design and fabrication of biosensors based on completely organic thin‐film transistors (OTFTs). The active material of the OTFTs is poly(9,9‐dioctylfluorene‐co‐bithiophene) (F8T2) polymer functionalized with biotin hydrazide. The relationship between the chemoresistive change and the binding of avidin‐biotin moieties in the polymer is observed in the output and on/off characteristics of the OTFTs. The exposure of the OTFTs to avidin causes a lowering of ID at VD = ‐40 V and VG = ‐40 V of nearly five orders of magnitude.

Part per Trillion Label-Free Electronic Bioanalytical Detection

“…27 Better performances were achieved with a biotinylated OS blend, capable of detecting 0.8 nM (0.05 ppm) of SA. 28 The best performance level reached was 10 pM (0.6 part per billion, ppb) SA detection obtained with an extremely sensitive system such as the biotinylated single silicon nanowire transistor is. 29 None of these studies involved the assessment of analytical figures of merit such as limit of detection, limit of quantification, analytical sensitivity, repeatability, or reproducibility.…”

“…Specifically, biotinylated lipid bilayers supported on carbon nanotube FETs have been proven to provide SA detections in the 5 μM or 312 part per million (mass fraction, ppm) range, while silicon nanowires transistor delivered 2.5 μM (156 ppm) . Better performances were achieved with a biotinylated OS blend, capable of detecting 0.8 nM (0.05 ppm) of SA . The best performance level reached was 10 pM (0.6 part per billion, ppb) SA detection obtained with an extremely sensitive system such as the biotinylated single silicon nanowire transistor is .…”

Biotin-Functionalized Semiconducting Polymer in an Organic Field Effect Transistor and Application as a Biosensor

“…The synthesized biotinylated final products were purified and the structures of the biotinylated F8T2 conducting polymers were analyzed. Our recent report describes the synthetic process of biotinylated F8T2-copolymers in detail [ 34 ].…”

“…These changes can be measured not only as changes of electrical signals, but also as changes in the color of UV-fluorescence. Previously, we reported some results of our research about organic thin film transistors in ETRI Journal [ 34 ]. In this report, we present some organic thin film transistors in which the conjugated organic semiconductor was used as the sensitive layer; we also describe a novel application of organic thin film transistors with organic semiconductors as the active sensitizing layer for the selective detection of biologically active species.…”

Copolythiophene-based water-gated organic field-effect transistors for biosensing

“…The electrical characterizations were performed in the dry state at quite high voltages (gate voltage V G ¼ À40 V). 25 Here, we report on the sensing of streptavidin and avidin by biotin-functionalized water-gated OFETs, using a random copolythiophene derived from P3HT as a semiconductor. This copolymer, P3HT-biotin, bears approximately 8% mol/mol of biotin groups.…”