Fabrication and characterization of andrographolide analogue (3A.1) nanosuspensions stabilized by amphiphilic chitosan derivatives for colorectal cancer therapy

Kansom, Teeratas; Sajomsang, Warayuth; Saeeng, Rungnapha; Rojanarata, Theerasak; Ngawhirunpat, Tanasait; Patrojanasophon, Prasopchai; Opanasopit, Praneet

doi:10.1016/j.jddst.2019.101287

Cited by 17 publications

(8 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hydrophobic phytochemicals may be loaded into the hydrophobic core of lipid NPs or emulsions, or hydrophobic compartments of other types of NPs. This leads to a significant enhancement in the solubility and stability of the hydrophobic phytochemicals [ 8 , 9 , 10 ]. In general, nanoencapsulation possesses the remarkable potential to significantly increase aqueous solubility, stability, and bioavailability.…”

Section: Stabilizers For Phytochemical-based Nps Preparation and Thei...mentioning

confidence: 99%

“…Thus, there exists a pressing imperative for researchers to explore innovative methodologies that can more proficiently augment the solubility and bioavailability of phytochemicals. Biocompatible and biodegradable nanoparticles (NPs) can be employed to overcome these issues [ 7 , 8 , 9 , 10 ]. Phytochemicals can be loaded into NPs, subsequently resulting in improved solubility, stability, and bioavailability [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Commonly Used Stabilizers for Phytochemical-Based Nanoparticles: Stabilization Effects, Mechanisms, and Applications

Zhou,

Peterson,

Fan

et al. 2023

Nutrients

View full text Add to dashboard Cite

Phytochemicals, such as resveratrol, curcumin, and quercetin, have many benefits for health, but most of them have a low bioavailability due to their poor water solubility and stability, quick metabolism, and clearance, which restricts the scope of their potential applications. To overcome these issues, different types of nanoparticles (NPs), especially biocompatible and biodegradable NPs, have been developed. NPs can carry phytochemicals and increase their solubility, stability, target specificity, and oral bioavailability. However, NPs are prone to irreversible aggregation, which leads to NP instability and loss of functions. To remedy this shortcoming, stabilizers like polymers and surfactants are incorporated on NPs. Stabilizers not only increase the stability of NPs, but also improve their characteristics. The current review focused on discussing the state of the art in research on synthesizing phytochemical-based NPs and their commonly employed stabilizers. Furthermore, stabilizers in these NPs were also discussed in terms of their applications, effects, and underlying mechanisms. This review aimed to provide more references for developing stabilizers and NPs for future research.

show abstract

Section: Stabilizers For Phytochemical-based Nps Preparation and Thei...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Commonly Used Stabilizers for Phytochemical-Based Nanoparticles: Stabilization Effects, Mechanisms, and Applications

Zhou,

Peterson,

Fan

et al. 2023

Nutrients

View full text Add to dashboard Cite

show abstract

“…For the formulations of 3nAG nanosuspensions (3nAGN) presented in Table 1, bottomup process technology was applied with the solvent-anti-solvent technique [61]. Firstly, the 3nAG and stabilizers were dissolved in 1.5 mL of DMSO as an organic solvent.…”

Section: Nag Nanosuspension Preparationmentioning

confidence: 99%

siRNA Targeting Mcl-1 Potentiates the Anticancer Activity of Andrographolide Nanosuspensions via Apoptosis in Breast Cancer Cells

et al. 2022

Self Cite

View full text Add to dashboard Cite

Breast cancer is the second leading cause of cancer-related death in the US. However, recurrence is frequently found despite adjuvant therapy being available. Combination therapy with cytotoxic drugs and gene therapy is being developed to be a new promising cancer treatment strategy. Introducing substituted dithiocarbamate moieties at the C12 position of andrographolide (3nAG) could improve its anticancer selectivity in the MCF-7 breast cancer cell line. However, its hydrophobicity is one of its main drawbacks. This work successfully prepared 3nAG nanosuspension stabilized with the chitosan derivative NSC (3nAGN-NSC) to increase solubility and pharmacological effectiveness. siRNAs have emerged as a promising therapeutic alternative for interfering with particular mRNA. The 3nAGN-NSC had also induced Mcl-1 mRNA expression in MCF-7 human breast cancer cells at 8, 12, and 24 h. This indicates that, in addition to Mcl-1 silencing by siRNA (siMcl-1) in MCF-7 with substantial Mcl-1 reliance, rationally devised combination treatment may cause the death of cancer cells in breast cancer. The Fa-CI analysis showed that the combination of 3nAGN-NSC and siMcl-1 had a synergistic effect with a combination index (CI) value of 0.75 (CI < 1 indicating synergistic effects) at the fractional inhibition of Fa 0.7. The synergistic effect was validated by flow cytometry, with the induction of apoptosis as the mechanism of reduced cell viability. Our findings suggested the rational use of 3nAGN-NSC in combination with siMcl-1 to kill breast cancer cells.

show abstract

“…Particularly, nano-based systems have been studied and reported, for example, polymeric nanoparticles [ 15 ], micelles [ 16 ], lipid-based nanoparticles [ 17 , 18 ], inorganic/metallic nanoparticles [ 19 , 20 ], nanoencapsulations [ 21 ], nanoemulsions [ 22 ], self-nanoemulsifying drug delivery systems [ 23 ], and hydrogels [ 24 ]. Moreover, AGP derivatives have been developed to increase bioavailability and druggability for various therapeutic purposes [ 25 , 26 ]. Although the development of advanced systems and technologies has shown to be significantly beneficial and promising, further pharmacokinetic and pharmacological examinations of these novel nano-based systems are crucial prior to clinical trials.…”

Section: Introductionmentioning

confidence: 99%

Development and Optimization of Andrographis paniculata Extract-Loaded Self-Microemulsifying Drug Delivery System Using Experimental Design Model

Pornpitchanarong,

Akkaramongkolporn,

Nattapulwat

et al. 2024

Pharmaceutics

Self Cite

View full text Add to dashboard Cite

The objectives of this study were to develop an optimized formulation for an Andrographis paniculata extract (AGPE)-loaded self-microemulsifying drug delivery system (SMEDDS) using an experimental design and evaluate the characteristics of the developed SMEDDS. The solubility of andrographolide (AGP) in various solvents was investigated. The pseudo-ternary phase was constructed to provide an optimal range for each component to form microemulsions (MEs). The formulation was optimized using an I-optimal design mixture type, where the physical stability, droplet size, polydispersity index, and zeta potential were examined. Soft capsules of the optimized AGPE-loaded SMEDDS were manufactured. The dissolution and ex vivo membrane permeation were studied. Oleic acid, Tween® 80, and PEG 400 were the best solubilizers for AGP. The promising surfactant to co-surfactant ratio to generate ME was 3:1. The optimized SMEDDS contained 68.998% Tween® 80, with 13.257% oleic acid and 17.745% PEG 400. The assayed content of AGP, uniformity of dosage unit, and stability complied with the expected specifications. The dissolution and membrane permeability of AGPE-loaded SMEDDS was significantly improved from the A. paniculata extract (p < 0.05). All in all, the developed optimized AGPE-loaded SMEDDS was proven to contain optimal composition and AGP content where a stable ME could spontaneously be formed with enhanced delivery efficacy.

show abstract

Fabrication and characterization of andrographolide analogue (3A.1) nanosuspensions stabilized by amphiphilic chitosan derivatives for colorectal cancer therapy

Cited by 17 publications

References 33 publications

The Commonly Used Stabilizers for Phytochemical-Based Nanoparticles: Stabilization Effects, Mechanisms, and Applications

The Commonly Used Stabilizers for Phytochemical-Based Nanoparticles: Stabilization Effects, Mechanisms, and Applications

siRNA Targeting Mcl-1 Potentiates the Anticancer Activity of Andrographolide Nanosuspensions via Apoptosis in Breast Cancer Cells

Development and Optimization of Andrographis paniculata Extract-Loaded Self-Microemulsifying Drug Delivery System Using Experimental Design Model

Contact Info

Product

Resources

About