Fabrication and Characterization of Cysteine-Functionalized Zinc Oxide Nanoparticles for Enzyme Immobilization

Verma, Nishant; Kumar, Nikhil; Upadhyay, Lata Sheo Bachan; Sahu, Reecha; Dutt, Aditya

doi:10.1080/00032719.2016.1245315

Cited by 18 publications

(8 citation statements)

References 30 publications

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“…The relative activity at various pH values was compared with the enzyme activity when adsorbed using native ZnO, which gave the highest relative activity of 68%. This value was comparable with the enzyme activity reported earlier for the immobilization of a cysteine functionalized enzyme immobilization using ZnO nanoparticles [ 71 ]. Unlike the green synthesized method, the least activity was observed at pH 7 for native ZnO.…”

Section: Resultssupporting

confidence: 91%

Green synthesis and characterization of zinc oxide nanoparticles using Cayratia pedata leaf extract

Jayachandran

Aswathy

Nair

2021

Biochemistry and Biophysics Reports

222

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The synthesis of Zinc oxide nanoparticles using a plant-mediated approach is presented in this paper. The nanoparticles were successfully synthesized using the Nitrate derivative of Zinc and plant extract of the indigenous medicinal plant Cayratia pedata. 0.1 mM of Zn (NO 3 ) 2 .6H 2 O was made to react with the plant extract at different concentrations, and the reaction temperature was maintained at 55 °C, 65 °C, and 75 °C. The yellow coloured paste obtained was wholly dried, collected, and packed for further analysis. In the UV visible spectrometer (UV–Vis) absorption peak was observed at 320 nm, which is specific for Zinc oxide nanoparticles. The characterization carried out using Field Emission Scanning Electron Microscope (FESEM) reveals the presence of Zinc oxide nanoparticles in its agglomerated form. From the X-ray diffraction (XRD) pattern, the average size of the nanoparticles was estimated to be 52.24 nm. Energy Dispersive Spectrum (EDX) results show the composition of Zinc and Oxygen, giving strong energy signals of 78.32% and 12.78% for Zinc and Oxygen, respectively. Fourier Transform - Infra-Red (FT-IR) spectroscopic analysis shows absorption peak of Zn–O bonding between 400 and 600 cm −1 . The various characterization methods carried out confirm the formation of nano Zinc oxide. The synthesized nanoparticles were used in the immobilization of the enzyme Glucose oxidase. Relative activity of 60% was obtained when Glucose oxidase was immobilized with the green synthesized ZnO nanoparticles. A comparative study of the green synthesized with native ZnO was also carried out. This green method of synthesis was found to be cost-effective and eco-friendly.

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Section: Resultssupporting

confidence: 91%

Green synthesis and characterization of zinc oxide nanoparticles using Cayratia pedata leaf extract

Jayachandran

Aswathy

Nair

2021

Biochemistry and Biophysics Reports

222

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“…To overcome these limitations, the use of zinc oxide (ZnO) for antibody immobilization in biosensors has been studied. − Some authors study biosensors for the enhancement of biomolecule detection in ZnO thin-film transistors . Other groups have reported ZnO nanosurfaces for C-reactive protein detection, cysteine-functionalized ZnO nanoparticles, using potato extract as a nontoxic and economical reducing agent, and electrochemical biosensors for detection of cardiac biomarkers, using the stoichiometric surface compositions of nanotextured ZnO thin films . However, the conventional techniques often used for ZnO thin-film deposition (sputtering, plasma-enhanced chemical vapor deposition, ablation laser, among others) have some disadvantages if low-cost platforms are required, such as expensive reagents, the need of high vacuum, and low compatibility with large-area substrates.…”

Section: Introductionmentioning

confidence: 99%

Antibody Immobilization in Zinc Oxide Thin Films as an Easy-Handle Strategy for Escherichia coli Detection

2020

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The antibody immobilization compatible with low-cost materials and label-free strategies is a challenge for biosensor device fabrication. In this study, ZnO thin film deposition was carried out on corning glass substrates by ultrasonic spray pyrolysis at 200 °C. The thin films were analyzed as platforms for enteropathogenic Escherichia coli ( E. coli EPEC) antibody immobilization. The modification of thin films from the functionalization and antibody immobilization steps was visualized using Fourier transform infrared spectroscopy (FTIR) spectroscopy, and surface changes were observed by atomic force microscopy. The obtained FTIR spectra after functionalization showed a contribution of the amino group (NH 2 ) derived from silane (3-aminopropyltrimethoxysilane). The antibody immobilization showed an amide I conserved signal corresponding to the C=O stretching vibrations and the amide II signal related to the N–H scissor vibration mode. In this way, the signals observed are correlated with the presence of antibody immobilized on the film. The ZnO film morphology changes after every stage of the process and allows observing the antibody distribution on the immobilized surface. In order to validate the antibody recognition capability as well as the E. coli EPEC detection in situ , polymerase chain reaction was used.

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“…The table shows that the results obtained using cysteine-functionalized ZrO 2 and SiO 2 materials as supports for laccase are satisfactory compared with the other results. As shown, cysteine has been used to modify Ag, Cu, ZnO and poly(glycidyl methacrylate)-SiO 2 , utilized as supports for alkaline phosphatase, urease and lipase, respectively [15,16,18,19]. Upadhyay et al [15] proposed a cysteine-Ag/AP biocatalytic system, which exhibited a specific activity of 6.31 U/mg and activity retention of 67%, and retained 60% of its initial activity after seven reaction cycles.…”

Section: Catalytic Properties Of the Obtained Biocatalytic Systemsmentioning

confidence: 99%

“…Upadhyay et al [15] proposed a cysteine-Ag/AP biocatalytic system, which exhibited a specific activity of 6.31 U/mg and activity retention of 67%, and retained 60% of its initial activity after seven reaction cycles. Kumar et al [16] and Verma et al [18] immobilized urease on cysteine-Cu and cysteine-ZnO, respectively. In both cases the activity retention was ca.…”

Section: Catalytic Properties Of the Obtained Biocatalytic Systemsmentioning

confidence: 99%

A Novel Cysteine-Functionalized MxOy Material as Support for Laccase Immobilization and a Potential Application in Decolorization of Alizarin Red S

Kołodziejczak-Radzimska

Jesionowski

2020

Processes

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Immobilization process improves the enzyme properties, like stability, activity, selectivity or specificity. In the study, a novel cysteine-functionalized MxOy (ZrO2, SiO2) material was used as a support for the immobilization of laccase from Trametes versicolor. The proposed matrix was prepared using a simple sol-gel method. The cysteine was introduced during the synthesis of a sample. Additionally, the obtained supports were modified with glutaraldehyde. The basic properties of the prepared cysteine functionalized ZrO2 and SiO2 were determined using spectroscopic, thermal, porous, electrostatic and elemental analysis. Furthermore, the obtained biocatalytic systems were used as catalysts in the oxidation of sulfonic acid. Catalytic and kinetic parameters were determined based on the proposed model reaction. Next, laccase immobilized on ZrO2- and SiO2-based materials were, for the first time, utilized in the decolorization of Alizarin Red S. In that process, the influence of duration, pH and temperature on the efficiency of decolorization was evaluated. The results show that the proposed biocatalytic systems offer good specific activity (ca. 19 U/mg) and activity retention (ca. 77%). Importantly, they can be successfully used in the decolorization of Alizarin Red S with high efficiency (above 95%).

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Fabrication and Characterization of Cysteine-Functionalized Zinc Oxide Nanoparticles for Enzyme Immobilization

Cited by 18 publications

References 30 publications

Green synthesis and characterization of zinc oxide nanoparticles using Cayratia pedata leaf extract

Green synthesis and characterization of zinc oxide nanoparticles using Cayratia pedata leaf extract

Antibody Immobilization in Zinc Oxide Thin Films as an Easy-Handle Strategy for Escherichia coli Detection

A Novel Cysteine-Functionalized MxOy Material as Support for Laccase Immobilization and a Potential Application in Decolorization of Alizarin Red S

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