The antibody immobilization compatible
with low-cost materials
and label-free strategies is a challenge for biosensor device fabrication.
In this study, ZnO thin film deposition was carried out on corning
glass substrates by ultrasonic spray pyrolysis at 200 °C. The
thin films were analyzed as platforms for enteropathogenic
Escherichia coli
(
E. coli
EPEC) antibody immobilization. The modification of thin films from
the functionalization and antibody immobilization steps was visualized
using Fourier transform infrared spectroscopy (FTIR) spectroscopy,
and surface changes were observed by atomic force microscopy. The
obtained FTIR spectra after functionalization showed a contribution
of the amino group (NH
2
) derived from silane (3-aminopropyltrimethoxysilane).
The antibody immobilization showed an amide I conserved signal corresponding
to the C=O stretching vibrations and the amide II signal related
to the N–H scissor vibration mode. In this way, the signals
observed are correlated with the presence of antibody immobilized
on the film. The ZnO film morphology changes after every stage of
the process and allows observing the antibody distribution on the
immobilized surface. In order to validate the antibody recognition
capability as well as the
E. coli
EPEC
detection
in situ
, polymerase chain reaction was
used.
In this work, Zinc Nitride (Zn3N2) based flexible thin film transistors (TFTs) are presented. The zinc nitride thin film is deposited by magnetron radio-frequency sputtering at room temperature, while spin-on glass and aluminum were used as gate insulator and source/drain electrode, respectively. Polyethylene terephthalate is used as flexible substrate. The flexible Zn3N2 TFTs were characterized while bent to 5 mm tensile radius. The flexible TFTs exhibit an electron mobility of 3.8 cm 2 /Vs and an on/off current ratio close to 10 5 after several cycles of bending and being exposed to air ambient for 30 days.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.