Fabrication and Characterization of Electrospun Folic Acid/Hybrid Fibers: In Vitro Controlled Release Study and Cytocompatibility Assays

Abstract

The fabrication of skin-care products with therapeutic properties has been significant for human health trends. In this study, we developed efficient hydrophilic composite nanofibers (NFs) loaded with the folic acid (FA) by electrospinning and electrospraying processes for tissue engineering or wound healing cosmetic applications. The morphological, chemical and thermal characteristics, in vitro release properties, and cytocompatibility of the resulting composite fibers with the same amount of folic acid were analyzed. The SEM micrographs indicate that the obtained nanofibers were in the nanometer range, with an average fiber diameter of 75–270 nm and a good porosity ratio (34–55%). The TGA curves show that FA inhibits the degradation of the polymer and acts as an antioxidant at high temperatures. More physical interaction between FA and matrices has been shown to occur in the electrospray process than in the electrospinning process. A UV-Vis in vitro study of FA-loaded electrospun fibers for 8 h in artificial acidic (pH 5.44) and alkaline (pH 8.04) sweat solutions exhibited a rapid release of FA-loaded electrospun fibers, showing the effect of polymer matrix–FA interactions and fabrication processes on their release from the nanofibers. PVA-CHi/FA webs have the highest release value, with 95.2% in alkaline media. In acidic media, the highest release (92%) occurred on the PVA-Gel–CHi/sFA sample, and this followed first-order and Korsmeyer–Peppas kinetic models. Further, the L929 cytocompatibility assay results pointed out that all NFs (with/without FA) generated had no cell toxicity; on the contrary, the FA in the fibers facilitates cell growth. Therefore, the nanofibers are a potential candidate material in skin-care and tissue engineering applications.

Development and in vitro characterization of capecitabine loaded biopolymeric vehicle for the treatment of colon cancer

“…The cell viability assay of optimized microsphere (F 6 ) was performed by (3‐[4,5‐dimethyl thiozol‐2yl]‐2,5 diphenyltetrazolium bromide) MTT assay on HT29 cell line 39,40 . Here only the viable cells were reduced MTT to the formazan crystals.…”

Electrospun Core–Sheath Nanofibers with Variable Shell Thickness for Modifying Curcumin Release to Achieve a Better Antibacterial Performance

“…Four mathematic models, such as first-order kinetic ( ), Higuchi ( ), Rigter–Peppas ( ), and zero-order kinetic ( ) model, were used to further process drug data [ 37 , 38 , 39 , 40 ]. where , , and are the initial drug amounts; k is the kinetic constant; n is the drug diffusion coefficient; and t is the time required for drug release.…”

Preparation of surface-functionalized electrospun PVA nanowebs for potential remedy for SARS-CoV-2

“…The fabrication of electrospun PVA/ZnO nanowebs from precursor PVA/ZnAc nanowebs have been studied for different purposes such as biomedical ( Jamnongkan et al, 2018 ), high-tech ( Wu et al, 2006 ) applications, and drug delivery ( Anero et al, 2021 ). The applications of functional electrospun PVA in transdermal drug delivery ( Parin et al, 2021a ), as bioactive fibers ( Parin et al, 2021b ), and in electronic skin textile ( Wang et al, 2021 ) have also been documented. Previous studies that explored the production of PVA/ZnO from precursor PVA/zinc acetate reported varying parameters specific for purpose.…”