Fabrication and characterization of graphene-based hybrid nanocomposite: assessment of antibacterial potential and biomedical application

Abstract

The present investigation deals with synthesis of graphene oxide (GO) and fabrication of GO-based hybrid nanocomposites (Ncs). Synthesized GO and Ncs were primarily confirmed by UV visible and Fourier transform infrared (FT-IR) spectroscopy. Fabricated Ncs showed potential antimicrobial activity against Gram-positive and Gram-negative bacterial strains. Surface morphology, Elemental analysis, and FTIR imaging analysis were carried out to confirm Ncs formation. The Ncs were impregnated into the pullulan polymeric layer-by-layer (LbL) ultrathin film by using novel spin-coating approach. Mechanical properties were determined using Brookfield texture analyzer, and percentage moisture content confirmed the physicochemical stability of LbL film.

A novel non-invasive strategy for low-level laser-induced cancer therapy by using new Ag/ZnO and Nd/ZnO functionalized reduced graphene oxide nanocomposites

“…The obtained results of ROS generation at dark condition showed a slight concentration-dependent increase in radical production for the as-synthesized NCs. However, previous studies have revealed that ZnO nanoparticles [51] and element-doped metal oxide [10] can be stimulated by radiation to produce ROS which destroy the cancerous cells.…”

“…In order to measure the amount of reactive oxygen species (ROS) and other free radicals, of as-synthesized NCs and pure ZnO NPs, a stable free radical scavenger DPPH was used in darkness, as previously described [10]. Briefly, to 2 ml of dispersed solutions of The NCs and ZnO NPs with concentrations (100, 200, 300, 400 and 500 lg/mL) in 96 well plates, 2 ml DPPH solution (8 mg powder DPPH in 100 ml methanol) was added.…”

“…DNA) or the normal cells environs the tumour cells [9]. Consequently, clinical management of patients as well as the need for assessment of therapies during different clinical trials, demonstrates the importance of radiation therapy for reducing injury to the normal cells [10]. Recently, the application of the materials in nanoscale as novel potential platforms has shown capability of drastically changing the cancer therapeutic systems.…”

“…ZnO, TiO 2 , Fe 3 O 4 , MnO 2 , SnO 2 , Co 3 O 4 ) [23,24]. In this regards, semiconductor nanoparticles (NPs) and quantum dots (QDs) look promising in cancer tracing/ treatment [10]. Based on scientific reports, ZnO is a renowned functional semiconductor oxide having wide-ranging direct band gap (3.37 eV, at room temperature) which have photodynamic property and produce free radical and singlet oxygen [25].…”

“…It also exhibits active therapeutic effect in photodynamic therapy and drug delivery [26]. The choice of dopants is crucial for a successful NIR irradiation with nanomaterials [10,21,22]. By keeping it in mind, in this work, both neodymium and silver nanoparticles (Nd and Ag NPs) were used as dopant in ZnO/rGO NCs for improving their effectiveness on treatment of deep tumours.…”