Fabrication and Characterization of Heparin/Collagen Sponge for in Vitro Differentiation of Wharton’s Jelly-Derived Mesenchymal Stem Cells into Hepatocytes

Aleahmad, Fatemeh; Talaei‐Khozani, Tahereh; Rajabi-Zeleti, Sareh; Sani, Mahsa; Jalili‐Firoozinezhad, Sasan; Bonakdar, Shahin; Heshmat-Azad, Sanaz; Azarnia, Mahnaz; Jaberipour, Mansoureh

doi:10.5812/hepatmon.40599

Cited by 7 publications

(8 citation statements)

References 43 publications

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“…As revealed in heparin release studies (Figure D), more than 60% of the immobilized heparin released from (PCL‐1ɑ)‐2hep fibrous membrane during the first 10 days of culture. Similar results was demonstrated in other research which by adding heparin more than 0.25 mg, the cell viability decreased remarkably . This result was due to the fact that the high contents of l ‐Iduronic acid (IdoA) and N ‐sulphonylated GlcN which are essential components in heparin molecules, correlated to its ability to inhibit cell proliferation .…”

Section: Resultssupporting

confidence: 87%

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Hemocompatible and Bioactive Heparin‐Loaded PCL‐α‐TCP Fibrous Membranes for Bone Tissue Engineering

Alehosseini

Golafshan

Kharaziha

et al. 2018

Macromolecular Bioscience

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The combination of bioactive components such as calcium phosphates and fibrous structures are encouraging niche-mimetic keys for restoring bone defects. However, the importance of hemocompatibility of the membranes is widely ignored. Heparin-loaded nanocomposite poly(ε-caprolactone) (PCL)-α-tricalcium phosphate (α-TCP) fibrous membranes are developed to provide bioactive and hemocompatible constructs for bone tissue engineering. Nanocomposite membranes are optimized based on bioactivity, mechanical properties, and cell interaction. Consequently, various concentrations of heparin molecules are loaded within nanocomposite fibrous membranes. In vitro heparin release profiles reveal a sustained release of heparin over the period of 14 days without an initial burst. Moreover, heparin encapsulation enhances mesenchymal stem cell (MSC) attachment and proliferation, depending on the heparin content. It is concluded that the incorporation of heparin within TCP-PCL fibrous membranes provides the most effective cellular interactions through synergistic physical and chemical cues.

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Section: Resultssupporting

confidence: 87%

“…Heparin is a bioactive material which helps immobilize the growth factors, and thus improves cell viability in vitro. However, the negative charges of heparin molecules have been suggested to inhibit cell proliferation . Therefore, the aim of this study was to evaluate the right concentration of heparin which can promote MCS proliferation.…”

Section: Resultsmentioning

confidence: 99%

Hemocompatible and Bioactive Heparin‐Loaded PCL‐α‐TCP Fibrous Membranes for Bone Tissue Engineering

Alehosseini

Golafshan

Kharaziha

et al. 2018

Macromolecular Bioscience

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“…Moreover, heparin is effective in decreasing inflammation (Vasavada, Praveen, Shah, Trivedi, & Vasavada, ), stimulating angiogenesis, and promoting hepatocyte proliferation (Yukawa et al, ). In addition, heparin an inherent anticoagulant—which is naturally in the Disse space (Aleahmad et al, ), as a sulfated glycosaminoglycan (GAG) has a high affinity to growth factors such as vascular endothelial growth factor (VEGF; Zhao, McCallum, Xiao, Zhang, & Linhardt, ) and hepatocyte growth factor (HGF; Liu, Zhang, Cheng, Lu, & Liu, ) and helps them to be preserved and stabilized. Heparin has a greatly negative charge and is covalently bound to collagen (Yang et al, ).…”

Section: Discussionmentioning

confidence: 99%

Decellularized liver transplant could be recellularized in rat partial hepatectomy model

Naeem

Daneshi

Talaei‐Khozani

et al. 2019

J Biomedical Materials Res

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In situ recellularization of the liver decellularized scaffold is a potential therapeutic alternative for liver transplantation. We aimed to develop an in situ procedure for recellularization of the rat liver using sodium lauryl ether sulfate (SLES) compared with Triton X-100/SDS. Rat liver specimens were rinsed with PBS, decellularized with either Triton X-100/SDS or SLES, and finally rinsed by distilled water. The efficiency of decellularized liver scaffolds was evaluated by histological, confocal Raman microscopy, histochemical staining, and DNA quantification assessments. Finally, in vivo studies were done to assess the biocompatibility of the liver scaffold by serum biochemical parameters and the recellularization capacity by histological and immunohistochemistry staining. Findings confirmed the preservation of extracellular matrix (ECM) components such as reticular, collagen, glycosaminoglycans, and neutral carbohydrates in both Triton X-100/SDS-and SLES-treated livers. Hoechst, feulgen, Hematoxylin and eosin, and DNA quantification assessments confirmed complete genetic content removal. The serological parameters showed no adverse impact on the liver functions. Transplantation of SLES-treated cell-free decellularized liver showed extensive neovascularization along with migration of the fibrocytes and adipocytes and some immune cells. Also, immunohistochemical staining showed that the oval cells, stellate cells, cholangiocytes and hepatocytes invaded extensively into the graft. It is concluded that SLES can be considered as a promising alternative in the liver decellularization process, and the transplanted decellularized liver can appropriately be revascularized and regenerated. K E Y W O R D S decellularization, liver, scaffold, sodium lauryl ether sulfate

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“…Composite biomaterials with two or more natural polymers have also been reported. The conjugation of heparin, which has a high affinity for a variety of growth factors, to a collagen scaffold led to enhanced hepatic differentiation of MSC as indicated by higher percentage of cells expressing cytokeratin 19 and ALB [ 74 ]. It is likely that heparin immobilizes growth factors such as hepatocyte growth factor, a known growth factor for hepatocyte differentiation, to present the signals locally.…”

Section: Biomaterials Systems Employed In Hepatic Differentiation Omentioning

confidence: 99%

Hepatic Differentiation of Stem Cells in 2D and 3D Biomaterial Systems

et al. 2020

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A critical shortage of donor livers for treating end-stage liver failure signifies the urgent need for alternative treatment options. Hepatocyte-like cells (HLC) derived from various stem cells represent a promising cell source for hepatocyte transplantation, liver tissue engineering, and development of a bioartificial liver assist device. At present, the protocols of hepatic differentiation of stem cells are optimized based on soluble chemical signals introduced in the culture medium and the HLC produced typically retain an immature phenotype. To promote further hepatic differentiation and maturation, biomaterials can be designed to recapitulate cell–extracellular matrix (ECM) interactions in both 2D and 3D configurations. In this review, we will summarize and compare various 2D and 3D biomaterial systems that have been applied to hepatic differentiation, and highlight their roles in presenting biochemical and physical cues to different stem cell sources.

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Fabrication and Characterization of Heparin/Collagen Sponge for in Vitro Differentiation of Wharton’s Jelly-Derived Mesenchymal Stem Cells into Hepatocytes

Cited by 7 publications

References 43 publications

Hemocompatible and Bioactive Heparin‐Loaded PCL‐α‐TCP Fibrous Membranes for Bone Tissue Engineering

Hemocompatible and Bioactive Heparin‐Loaded PCL‐α‐TCP Fibrous Membranes for Bone Tissue Engineering

Decellularized liver transplant could be recellularized in rat partial hepatectomy model

Hepatic Differentiation of Stem Cells in 2D and 3D Biomaterial Systems

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