In this study, PEGylated locust bean gum-rosin glycerol ester polymeric nanoparticles (PEG-LBG/RE PNPs) were synthesized by using simple ultrasonic irradiation method. The nanoparticles were characterized by using Fouriertransform infrared spectroscopy (FTIR) and scanning transmission electron microscopy (STEM). The viscosity behaviors of nanoparticles were studied in different conditions (pH, sonication time, and salt). The experimental results were calculated by Huggins, Kraemer, Tanglertpaibul-Rao, and Higiro models to understand the colloidal stability, the miscibility mechanism, and coefficients of nanoparticles. The results confirmed that the homogenous distribution of nanostructure was related to sonication time (30 min) and the presence of NaOH salt. With the addition of NaOH, the nanosystem based on ionotropic gelation technique was made more homogeneous. The results made us think that nanoparticles can be a good candidate for drug delivery systems in biomedical and pharmaceutical applications.