Fabrication and Characterization of Plasma-Polymerized Poly(ethylene glycol) Film with Superior Biocompatibility

Abstract

A newly fabricated plasma-polymerized poly(ethylene glycol) (PP-PEG) film shows extremely low toxicity, low fouling, good durability, and chemical similarity to typical PEG polymers, enabling live cell patterning as well as various bioapplications using bioincompatible materials. The PP-PEG film can be overlaid on any materials via the capacitively coupled plasma chemical vapor deposition (CCP-CVD) method using nontoxic PEG200 as a precursor. The biocompatibility of the PP-PEG-coated surface is confirmed by whole blood flow experiments where no thrombi and less serum protein adsorption are observed when compared with bare glass, polyethylene (PE), and polyethylene terephthalate (PET) surfaces. Furthermore, unlike bare PE films, less fibrosis and inflammation are observed when the PP-PEG-coated PE film is implanted into subcutaneous pockets of mice groin areas. The cell-repellent property of PP-PEG is also verified via patterning of mammalian cells, such as fibroblasts and hippocampal neurons. These results show that our PP-PEG film, generated by the CCP-CVD method, is a biocompatible material that can be considered for broad applications in biomedical and functional materials fields.

Deposition of Non-Fouling PEO-Like Coatings Using a Low Temperature Atmospheric Pressure Plasma Jet

“…The monomer was introduced into the plasma discharge at increasing flow rates on heated substrates. Peaks observed at 1 250–1 400 cm −1 are associated with various wagging, twisting and bending modes of CH 2 on the TEGDME [CH 2 CH 2 O] n subunit . The intensity of CH 2 and CH 3 groups is decreasing with an associated increasing in CO (1 735 cm −1 ) groups, which are not observed in the original monomer spectrum, i.e., original groups are dehydrogenised and oxidised, indicative of monomer fragmentation and polymerisation reactions.…”

“…FTIR analysis of films deposited from TEGDME can be seen in Figure . Absorption peaks identified in the plasma polymerised spectra of TEGDME correspond with previous reported studies . The monomer spectrum is dominated by the COC stretching mode at 1 110 cm −1 and CH 2 , CH 3 stretching modes from 2 735 to 2 976 cm −1 .…”

Enhanced cytocompatibility of silver-containing biointerface by constructing nitrogen functionalities

“…In this work, Ag and N 2 co-PIII is performed to enhance both cytocompatibility and antibacterial properties. When the materials are exposed to cells suspended in a culture medium supplemented with FBS, proteins in the serum are rapidly adsorbed and the adsorbed proteins play a large role in the subsequent cell behavior [31]. Ag PIII and Ag/N 2 PIII increase the absorption ability of the BSA protein (Fig.…”

Surface Plasmon‐Based Techniques for the Analysis of Plasma Deposited Functional Films and Surfaces

“…Functional polymers, which could resist the non‐specific adsorption of proteins, are highly desirable for many biomedical applications. Plasma deposition has proved to be an effective method for the fabrication of non‐fouling surfaces . SPR technique is powerful method to monitor the kinetic adsorption process, as well as, to provide the amount of adsorbed proteins .…”