Fabrication and Characterization of Polyvinylpyrrolidone-Eggshell Membrane-Reduced Graphene Oxide Nanofibers for Tissue Engineering Applications

Abstract

One of the best methods to prevent wound infection and speed up wound healing is wound dressing based on nanofiber–polymer scaffolds, which have acceptable antimicrobial performance and appropriate skin regeneration capabilities. In this paper, the electrospinning method was applied to synthesize the polyvinylpyrrolidone-acrylic acid hydrogel (PVPA)–eggshell membrane (ESM)–reduced graphene oxide (rGO) nanosheets nanocomposite dressings with different reduced graphene oxide contents (0, 0.5, 1, and 2 wt.%). Thus, smooth nanofibers were fabricated, including a high amount of rGO, which reduced the fiber diameter. Based on the results, rGO played an important role in water impermeability. The results showed that by increasing the rGO concentration from 0.5 to 2 wt%, the contact angle value increased persistently. Results showed that compared to PVPA–ESM, the mechanical strength and strain of PVPA–ESM/1 wt% rGO significantly enhanced 28% and 23%, respectively. Incorporation of 1 wt% rGO enhanced swelling ratio from 875% for PVPA-ESM to 1235% after 420 min, while increasing the rGO to 2 wt% increased the degradation rate of the composites. According to the in vitro cell culture studies, PVPA-ESM wound dressings with 0.5–1 wt% rGO content enhanced PC12 cell viability compared to the wound dressings without rGO nanosheets. Generally, rGO–loaded PVPA-ESM nanofiber wound dressing can be considered as a potential candidate to be used in skin regeneration applications.

Preparation of p-Phenylenediamine Modified Graphene Foam/Polyaniline@Epoxy Composite with Superior Thermal and EMI Shielding Performance

“…The spectra for GO show a typical broad absorption band at 3525 cm −1 , which is assigned to the stretching vibration of the hydroxyl groups [ 26 ]. The peaks at 1779 cm −1 , 1616 cm −1 , 1220 cm −1 , and 1026 cm −1 corresponded to the C=O stretching vibration in carboxylic groups, bending vibration of hydroxyl and the stretching vibration of epoxide and alkoxy in GO, respectively [ 27 , 28 ]. After the hydrothermal process to obtain GF, the peak intensity at 1779 cm −1 and 1616 cm −1 get decreased, indicating the partially reduction in GO.…”

The chicken eggshell membrane: a versatile, sustainable, biological material for translational biomedical applications

“…To overcome this challenge, an alternative strategy has been proposed which is based on the dissolution of the CaCO 3 -containing shell by placing the egg into highly acidic solution. The most common are acetic acid, hydrochloric acid (HA) and ethylenediaminetetraacetic acid (EDTA) (Farjah et al 2013, Mensah et al 2021, Sheish et al 2021. Mensah et al (2021) showed that the acid treatment resulted in thicker and more porous membranes compared to the manual stripping.…”

Recent biomedical advancements in graphene oxide- and reduced graphene oxide-based nanocomposite nanocarriers

“…Both GO and rGO encourage stem cell proliferation and differentiation while functioning as scaffolds or parts of a scaffold, making them ideal for tissue engineering and regeneration, the various applications of which are listed in Table 5 . While GO and rGO nanoparticles can be incorporated into scaffolds for general tissue engineering, such as skin [ 188 – 190 ], cartilage [ 191 , 192 ], bone [ 189 , 193 – 195 ], and muscle tissue [ 196 , 197 ] rGO is commonly incorporated into nanofibers to enhance their electroconductivity, which is a significant factor in cardiac and nerve tissue regeneration [ 39 – 41 ]. Tissue engineering applications can also implement GO/rGO for morphological [ 198 ] and photoelectric [ 199 ] stimulations to encourage cell proliferation/differentiation.…”