Fabrication and Characterization of Reconstituted Silk Microgels for the Storage and Release of Small Molecules

Liu, Xizhou; Toprakcioglu, Zenon; Dear, Alexander J.; Levin, Aviad; Ruggeri, Francesco Simone; Taylor, Christopher G.; Hu, Mengsha; Kumita, Janet R.; Andreasen, Maria; Dobson, Christopher M.; Shimanovich, Ulyana; Knowles, Tuomas P. J.

doi:10.1002/marc.201800898

Cited by 32 publications

(36 citation statements)

References 82 publications

(145 reference statements)

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“…Similarly, studies by Liu et al [59], developed polymeric nanoparticles containing poly(lactic-co-glycolic acid) (PLGA) to encapsulate the bioactive component curcumin and evaluate its antimicrobial capacity. It was observed that to have a significant reduction in E. coli viability required low concentrations of curcumin compared to the free bioactive and this was due to improved solubility and ability to direct curcumin bacteria.…”

Section: Antimicrobial Activity Of Nanoparticlesmentioning

confidence: 99%

Polymeric Nanoparticle Associated with Ceftriaxone and Extract of Schinopsis Brasiliensis Engler against Multiresistant Enterobacteria

et al. 2020

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Bacterial resistance has become an important public health problem. Bacteria have been acquiring mechanisms to resist the action of antimicrobial active pharmaceutical ingredients (API). Based on this, a promising alternative is the use of nanotechnology, since when the systems are presented in nanometric size, there is an increase in the interaction and concentration of the action at the target site improving the activity. Thus, this study aims to develop a polymeric nanoparticle (PN) composed of chitosan and hydroxypropylmethylcellulose, as an innovative strategy for the administration of an association between ceftriaxone and extract of S. brasiliensis, for the treatment of Enterobacteriaceae. From a Box–Behnken design, nanoparticles were obtained and evaluated using the DLS technique, obtaining the particle size between 440 and 1660 nm, IPD from 0.42 to 0.92, and positive charges. Morphological characteristics of PN by SEM revealed spherical morphology and sizes similar to DLS. Infrared spectroscopy showed no chemical interaction between the components of the formulation. The broth microdilution technique evaluated their antimicrobial activity, and a considerable improvement in the activity of the extract and the API compared to the free compounds was found, reaching an improvement of 133 times in the minimum inhibitory activity CRO.

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Section: Antimicrobial Activity Of Nanoparticlesmentioning

confidence: 99%

Polymeric Nanoparticle Associated with Ceftriaxone and Extract of Schinopsis Brasiliensis Engler against Multiresistant Enterobacteria

et al. 2020

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“…Silks represent a distinctive group of structural protein that are degradable, biocompatible, eco‐friendly, and mechanically superior, and are proposed as promising biomedical materials . Various types of silk fibroin‐based materials, such as films, fibers, scaffolds, and gels, can be obtained through dissolving silk filaments and making regenerated silk fibroin (RSF) solutions . Physically crosslinked RSF hydrogels are often more brittle than the common chemically cross‐linked hydrogels.…”

mentioning

confidence: 99%

Highly Stretchable and Tough Physical Silk Fibroin–Based Double Network Hydrogels

Zhao

Guan

2019

Macromol. Rapid Commun.

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a facile way to construct strong RSF hydrogels simply by adding sodium dodecyl sulfate (SDS) into high molecular weight RSF solutions. The resulting hydrogels demonstrated high tensile strength of ≈0.74 MPa and fair extensibility of 140%. [6] Shao et al. also found that ionic liquid (IL)/water mixtures could generate RSF-based gels, whose tensile strength and breaking elongation reached up to 0.4 MPa and 147%, respectively. [11] Okay et al. reported a novel strategy to design mechanically robust and stretchable silk fibroin/hyaluronic acid hydrogels with chemical cross-links and physical cross-links (β-sheet domains). [7] The hybrid hydrogels exhibited a high extensibility up to around 400% and tensile strength of 65 kPa after equilibrium swelling. Okay also described a directional freezing/cryogelation method to produce high-strength silk fibroin cryogels with a degree of mechanical anisotropy. The compressive modulus of cryogels formed at 16.7 wt% RSF concentration was 45 MPa, but the tensile properties were not shown. [12] Consequently, achieving highly stretchable RSF hydrogels for wide-range applications [13] is still in its infancy.An alternative strategy to prepare strong and tough hydrogels is the double network (DN) hydrogel, which introduces a flexible and stretchable second network. These DN hydrogels demonstrated high fracture strength (0.1-10 MPa) and high water content (60-90%). [14][15][16][17][18][19] A series of DN gels using biomacromolecules (agar, cellulose) had been developed. [20,21] Chen et al. developed RSF-based DN hydrogels via introducing a hydrophobically associated gel of polyacrylamide (HPAAm) as the second network. [10] The DN hydrogels were conveniently synthesized by the one-pot method coupled with photo-polymerization, and the resultant RSF/HPAAm DN gels showed a tensile strength of ≈1.17 MPa, and breaking elongation of ≈1900%, but non-equilibrium RSF/HPAAm DN gels contained excessive residual monomers. It is notable that although the RSF gels could be rapidly achieved via introducing SDS, the gelation that takes only a few minutes is difficult to control. It is quite difficult to achieve uniform mixing between SDS micelles and the hydrophobic monomers via the one-step method, which often resulted in poor homogeneity of the gels. In addition, the swelling of as-prepared hydrogels in water destroys the RSF/SDS network so that equilibrium hydrogels cannot be obtained. In contrast, if the first RSF/SDS network can be stabilized first during the swelling of hydrogels, the Regenerated silk fibroin (RSF) is a promising biomedical material, but the poor mechanical properties of RSF hydrogels may hinder the use as structural components. Herein, an equilibrium RSF hydrogel is prepared and optimized based on the double network (DN) concept. After sufficient soaking in water and removal of small molecules, the equilibrium RSF DN hydrogels prove stable in water, strong, highly extensible, and tough with 0.26-0.44 MPa tensile strength, 500-900% elongation, and 2 MJ m −3 work of extension. The...

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“…Microparticles were confined using a microfluidic trapping array. 33 First, the whole device was filled with buffer and all possible bubbles were erased. Next, microparticles in buffer were inserted into the device through the denoted inlet.…”

Section: Methodsmentioning

confidence: 99%

Continuous Flow Reactors from Microfluidic Compartmentalization of Enzymes within Inorganic Microparticles

Hakala

Bialas

Toprakcioglu

et al. 2020

ACS Appl. Mater. Interfaces

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Compartmentalization and selective transport of molecular species are key aspects of chemical transformations inside the cell. In an artificial setting, the immobilization of a wide range of enzymes onto surfaces is commonly used for controlling their functionality but such approaches can restrict their efficacy and expose them to degrading environmental conditions, thus reducing their activity. Here, we employ an approach based on droplet microfluidics to generate enzyme-containing microparticles that feature an inorganic silica shell that forms a semipermeable barrier. We show that this porous shell permits selective diffusion of the substrate and product while protecting the enzymes from degradation by proteinases and maintaining their functionality over multiple reaction cycles. We illustrate the power of this approach by synthesizing microparticles that can be employed to detect glucose levels through simultaneous encapsulation of two distinct enzymes that form a controlled reaction cascade. These results demonstrate a robust, accessible, and modular approach for the formation of microparticles containing active but protected enzymes for molecular sensing applications and potential novel diagnostic platforms.

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Fabrication and Characterization of Reconstituted Silk Microgels for the Storage and Release of Small Molecules

Cited by 32 publications

References 82 publications

Polymeric Nanoparticle Associated with Ceftriaxone and Extract of Schinopsis Brasiliensis Engler against Multiresistant Enterobacteria

Polymeric Nanoparticle Associated with Ceftriaxone and Extract of Schinopsis Brasiliensis Engler against Multiresistant Enterobacteria

Highly Stretchable and Tough Physical Silk Fibroin–Based Double Network Hydrogels

Continuous Flow Reactors from Microfluidic Compartmentalization of Enzymes within Inorganic Microparticles

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