Fabrication and Permeability Characteristics of Microdialysis Probe Using Chitosan Nanoporous Membrane

Abstract

In this article, a nanoporous chitosan polymer membrane was successfully produced and applied as microdialysis membrane forin vitrosampling of biomolecules. With the use of nanoparticle leaching technique, porogenic gelatin nanoparticles formed nanopores in the chitosan-based membrane to create a secure implantable nanoporous membrane for biomolecule sampling. The gelatin nanoparticles size was in the range of 45 to 70 nm, and the pore size of the chitosan membrane was around 40 to 100 nm. The porosity of membrane was found to be dependent on the mixing ratio of chitosan solution and gelatin nanoparticles solution. The results of diffusion study showed that we can alter the mixing ratio of porogen to achieve size-selective molecular diffusion, which means that the porosity and cut-off size of porous membrane can be controlled. The recoveries of the probe fabricated from the chitosan-based membrane were examined for four different model compounds of different molecular weights: 2-NBDG, substance P, TNF-α, and FITC-BSA. The microdialysis probes showed linear responses and substantial recovery to various concentrations of biomolecules. These results indicated that the microdialysis probe constructed by chitosan nanoporous membrane could sample and monitor the biomoleculesin vitroand has the potential for the applicationin vivo.

Extended (10-Day) Real-Time Monitoring by Dexamethasone-Enhanced Microdialysis in the Injured Rat Cortex

“…We wish to be duly cautious in interpreting the progressive glucose decline. We must consider the possibility that it is related to the permeability of the microdialysis membrane. , Whereas data from control animals shows that microdialysis probes continue to recover glucose from the brain for up to 10 days, it is possible that CCI triggers a more intense inflammatory response to probe insertion, resulting in a loss of glucose recovery. Several observations speak against this possibility.…”

Chitosan-Hydroxyapatite Bio-Based Composite in Film Form: Synthesis and Application in Wastewater

“…This leads to the conclusion that the adsorption process was a mixed process. The liquid film diffusion model, which assumes the transport of cadmium and zinc through a liquid film covering the solid adsorbent, is another kinetic model [ 40 ]. The liquid film diffusion model may be expressed using Equation (11).…”

“…In this procedure, a chitosan solution in 5% aqueous acetic acid was prepared. A separate solution of HAp was prepared by dissolving chitosan in a 5% aqueous solution of acetic acid [ 22 , 23 , 40 , 41 ]. The two solutions were then mixed with stirring at 45 °C for 2 h. The resulting solution’s pH was then adjusted to 10.5 using ammonia and stirring was maintained at 60 °C for another 12 h before being decanted onto a petri plate and left at room temperature for 48 h, and a composite in the form of a transparent film was produced.…”