Fabrication, Characterization and In Vitro Cell Cytotoxicity of ZnO Nanoparticles on MDA‐MB 231 Breast Cancer Cell Line

Abstract

The intention of this study was to prepare zinc oxide nanoparticles (ZnO‐NPs) via bottom‐up approach and evaluate their physiochemical, antimicrobial and anticancer properties. ZnO‐NPs were first prepared by the direct precipitation method with zinc sulphate heptahydrate, followed by characterization tests using sophisticated analytical and imaging instruments. Upon characterization, ZnO‐NPs were studied for their antimicrobial activities against Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa as well as their anticancer activity against breast cancer cell line (MDA‐MB 231). The ZnO‐NPs possessed a mean particle diameter of 225.2 nm and a zeta potential of −15.8 mV. A sharp absorption peak of ZnO was recorded at 354 nm via UV‐Visible spectroscopy. Hexagonal wurtzite shape of ZnO‐NPs has been observed and confirmed by Scanning Electron Microscopy (SEM) and X‐Ray diffraction (XRD). ZnO‐NPs exhibited excellent antimicrobial activity against the Gram‐positive bacteria, Staphylococcus aureus. In an in vitro cell cytotoxicity study, ZnO‐NPs showed a dose‐dependent relationship against MDA‐MB 231 breast cancer cells. It was observed that as the concentration of ZnO‐NPs increased (0.25 μg to 35 μg), there was a sharp reduction in the proliferation rate of the cancer cell line. Overall, our study reported successful synthesis of ZnO‐NPs, which can be efficient antibacterial and anticancer agents.

Application of 32 factorial design for loratadine-loaded nanosponge in topical gel formulation: comprehensive in-vitro and ex vivo evaluations

“…Also, the spreadability, viscosity, and pH of LoR-NS gel were stable in the same experimental conditions. The data obtained with LoR-NS suspension corroborate previously published data 38 . There was no significant difference ( p > 0.05) in stability after 2 months, and the slight changes observed in its pH, %CDR, viscosity and % EE but not significantly difference after 90 days as given in Table 5 and Fig.…”

ZnO nanoparticles: A sustainable fabrication enhanced by eggshell waste and its germicidal, anti‐proliferative, and photodecomposition properties

“…The findings of our study were confirmed by Ramya et al, 5 who documented significant cytotoxicity at lower doses when utilizing biological TiO 2 NPs, owing to their fascinating attributes. Prior studies have consistently shown that ZnO nanoparticles can induce harm to a range of cell types, including the human MDA‐MB 231 Breast Cancer Cell Lines, SW480 Cell Lines, A549 cancer cells, and MG‐63 cells Ashokkumar et al, 30 Marjan et al, 31 Tamil Elakiya et al, 23 and Jun et al 32 …”