Fabrication, modeling and optimization of lyophilized advanced platelet rich fibrin in combination with collagen-chitosan as a guided bone regeneration membrane

Ansarizadeh, Mohamadhasan; Mashayekhan, Shohreh; Saadatmand, Maryam

doi:10.1016/j.ijbiomac.2018.12.078

Cited by 22 publications

(16 citation statements)

References 56 publications

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“…Deviations from the published protocols can result in variations of physical properties and biological components as seen in this study and in others [17]. In addition, further research to develop a novel biomaterial product from i-PRF is an interesting topic, where the freeze-dried or lyophilized technique may be used for sterilization [36,37].…”

Section: Discussionmentioning

confidence: 76%

Influence of Fractionation Methods on Physical and Biological Properties of Injectable Platelet-Rich Fibrin: An Exploratory Study

Thanasrisuebwong

Surarit

Bencharit

et al. 2019

IJMS

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Injectable platelet-rich fibrin (i-PRF) has been used as an autografting material to enhance bone regeneration through intrinsic growth factors. However, fractionation protocols used to prepare i-PRF can be varied and the effects of different fractionation protocols are not known. In this study, we investigated the influence of different fractions of i-PRF on the physical and biological properties derived from variations in i-PRF fractionation preparation. The i-PRF samples, obtained from the blood samples of 10 donors, were used to harvest i-PRF and were fractioned into two types. The yellow i-PRF fractionation was harvested from the upper yellow zone, while the red i-PRF fractionation was collected from both the yellow and red zone of the buffy coat. The viscoelastic property measurements, including the clot formation time, α-angle, and maximum clot firmness, were performed by rotational thromboelastometry. The fibrin network was examined using a scanning electron microscope. Furthermore, the concentration of growth factors released, including VEGF, TGF-β1, and PDGF, were quantified using ELISA. A paired t-test with a 95% confidence interval was used. All three viscoelastic properties were statistically significantly higher in the yellow i-PRF compared to the red i-PRF. The scanning electron microscope reviewed more cellular components in the red i-PRF compared to the yellow i-PRF. In addition, the fibrin network of the yellow i-PRF showed a higher density than that in the red i-PRF. There was no statistically significant difference between the concentration of VEGF and TGF-β1. However, at Day 7 and Day 14 PDGF concentrations were statistically significantly higher in the red i-PRF compared to the yellow group. In conclusion, these results showed that the red i-PRF provided better biological properties through the release of growth factors. On the other hand, the yellow i-PRF had greater viscoelastic physical properties. Further investigations into the appropriate i-PRF fractionation for certain surgical procedures are therefore necessary to clarify the suitability for each fraction for different types of regenerative therapy.

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Section: Discussionmentioning

confidence: 76%

Influence of Fractionation Methods on Physical and Biological Properties of Injectable Platelet-Rich Fibrin: An Exploratory Study

Thanasrisuebwong

Surarit

Bencharit

et al. 2019

IJMS

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“…Ansarizadeh et al recently described the mechanistic properties of collagen-chitosan membrane activated with lyophilized PRF. They found a decreased degradation rate of the membranes from 90 to 20% after 4 weeks and furthermore an effect of the membrane on osseous differentiation in vitro [59]. Consequently, the approach described in this manuscript should be further evaluated on mechanical characters such as degradation rate and a possible effect on osteoblast differentiation and proliferation (and therefore hard tissue regeneration) in future studies.…”

Section: Discussionmentioning

confidence: 88%

Biofunctionalization of porcine-derived collagen matrices with platelet rich fibrin: influence on angiogenesis in vitro and in vivo

et al. 2020

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Objectives Porcine-derived collagen matrices (CM) can be used for oral tissue regeneration, but sufficient revascularization is crucial. The aim of this study was to analyze the influence of platelet-rich fibrin (PRF) on angiogenesis of different CM in vitro and in vivo. Materials and methods Three different CM (mucoderm®, jason®, collprotect®) were combined with PRF in a plotting process. Growth factor release (VEGF, TGF-β) was measured in vitro via ELISA quantification after 1,4 and 7 days in comparison to PRF alone. In ovo yolk sac (YSM) and chorion allantois membrane (CAM) model, angiogenic potential were analyzed in vivo with light-and intravital fluorescence microscopy after 24 h, then verified with immunohistochemical staining for CD105 and αSMA. Results Highest growth factor release was seen after 24 h for all three activated membranes in comparison to the native CM (VEGF 24 h: each p < 0.05; TGF-β: each p < 0.001) and the PRF (no significant difference). All activated membranes revealed a significantly increased angiogenic potential in vivo after 24 h (vessels per mm 2 : each p < 0.05; branching points per mm 2 : each p < 0.01; vessel density: each p < 0.05) and with immunohistochemical staining for CD105 (each p < 0.01) and αSMA (each p < 0.05). Conclusions PRF improved the angiogenesis of CM in vitro and in vivo. Clinical relevance Bio-functionalization of CM with PRF could easily implemented in the clinical pathway and may lead to advanced soft tissue healing.

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“…Accordingly, a randomized clinical trial, comparing FD-PRF vs. fresh PRF in guided bone regeneration surgery did not find any difference in their clinical performance [70]. An optimized membrane, in terms of biological activities and mechanical properties, was elaborated with FD-PRF/chitosan/collagen [71] to be used in periodontal disease. A biodegradable polymer mesh coated with FD-PRP was effective in promoting periodontal ligament cell adhesion and growth as well as stimulating angiogenic mechanisms [72].…”

Section: Dentistry Researchmentioning

confidence: 99%

Freeze-Drying of Platelet-Rich Plasma: The Quest for Standardization

Andı́a

Perez‐Valle

Amo

et al. 2020

IJMS

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The complex biology of platelets and their involvement in tissue repair and inflammation have inspired the development of platelet-rich plasma (PRP) therapies for a broad array of medical needs. However, clinical advances are hampered by the fact that PRP products, doses and treatment protocols are far from being standardized. Freeze-drying PRP (FD-PRP) preserves platelet function, cytokine concentration and functionality, and has been proposed as a consistent method for product standardization and fabrication of an off-the-shelf product with improved stability and readiness for future uses. Here, we present the current state of experimental and clinical FD-PRP research in the different medical areas in which PRP has potential to meet prevailing medical needs. A systematic search, according to PRISMA (Preferred Reported Items for Systematic Reviews and Meta-Analyses) guidelines, showed that research is mostly focused on wound healing, i.e., developing combination products for ulcer management. Injectable hydrogels are investigated for lumbar fusion and knee conditions. In dentistry, combination products permit slow kinetics of growth factor release and functionalized membranes for guided bone regeneration.

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Fabrication, modeling and optimization of lyophilized advanced platelet rich fibrin in combination with collagen-chitosan as a guided bone regeneration membrane

Cited by 22 publications

References 56 publications

Influence of Fractionation Methods on Physical and Biological Properties of Injectable Platelet-Rich Fibrin: An Exploratory Study

Influence of Fractionation Methods on Physical and Biological Properties of Injectable Platelet-Rich Fibrin: An Exploratory Study

Biofunctionalization of porcine-derived collagen matrices with platelet rich fibrin: influence on angiogenesis in vitro and in vivo

Freeze-Drying of Platelet-Rich Plasma: The Quest for Standardization

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