Fabrication of 3D freeform porous tubular constructs with mechanical flexibility mimicking that of soft vascular tissue

Lee, Ji Eun; Park, Seong Je; Yoon, Yeji; Son, Yong; Park, Suk-Hee

doi:10.1016/j.jmbbm.2018.12.020

Cited by 30 publications

(31 citation statements)

References 24 publications

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“…In contrast, our graft is soft and alleviates the mechanical mismatch between graft and native tissue. The 3DT graft is expected to provide great potential as a biomimetic vascular graft owing to its mechanical softness, which has been continuously challenged in previous studies [17,39,40]. The cytotoxicity of our synthetic 3DT is not different from that of the commercially available SG.…”

Section: Discussionmentioning

confidence: 94%

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Evaluation of 3D Templated Synthetic Vascular Graft Compared with Standard Graft in a Rat Model: Potential Use as an Artificial Vascular Graft in Cardiovascular Disease

Sohn

Kim

et al. 2021

Materials

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Although the number of vascular surgeries using vascular grafts is increasing, they are limited by vascular graft-related complications and size discrepancy. Current efforts to develop the ideal synthetic vascular graft for clinical application using tissue engineering or 3D printing are far from satisfactory. Therefore, we aimed to re-design the vascular graft with modified materials and 3D printing techniques and also demonstrated the improved applications of our new vascular graft clinically. We designed the 3D printed polyvinyl alcohol (PVA) templates according to the vessel size and shape, and these were dip-coated with salt-suspended thermoplastic polyurethane (TPU). Next, the core template was removed to obtain a customized porous TPU graft. The mechanical testing and cytotoxicity studies of the new synthetic 3D templated vascular grafts (3DT) were more appropriate compared with commercially available polytetrafluoroethylene (PTFE) grafts (ePTFE; standard graft, SG) for clinical use. Finally, we performed implantation of the 3DTs and SGs into the rat abdominal aorta as a patch technique. Four groups of the animal model (SG_7 days, SG_30 days, 3DT_7 days, and 3DT_30 days) were enrolled in this study. The abdominal aorta was surgically opened and sutured with SG or 3DT with 8/0 Prolene. The degree of endothelial cell activation, neovascularization, thrombus formation, calcification, inflammatory infiltrates, and fibrosis were analyzed histopathologically. There was significantly decreased thrombogenesis in the group treated with the 3DT for 30 days compared with the group treated with the SG for 7 and 30 days, and the 3DT for 7 days. In addition, the group treated with the 3DT for 30 days may also have shown increased postoperative endothelialization in the early stages. In conclusion, this study suggests the possibility of using the 3DT as an SG substitute in vascular surgery.

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Section: Discussionmentioning

confidence: 94%

“…We previously reported a template-based fabrication of tubular tissue engineering scaffolds [17,18]. In this study, using the same process, the artificial vascular grafts were fab-ricated and customized using a 3D printing technique.…”

Section: D Customized Artificial Vascular Graftsmentioning

confidence: 99%

Evaluation of 3D Templated Synthetic Vascular Graft Compared with Standard Graft in a Rat Model: Potential Use as an Artificial Vascular Graft in Cardiovascular Disease

Sohn

Kim

et al. 2021

Materials

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“…Mechanical properties are key factors that affect tissue regeneration in addition to biocompatibility. Matching of the Young's modulus of the implant material with that of the tissue can reduce complications such as tissue damage, inflammation, and necrosis and contribute to cell adhesion and tissue regeneration (Lee et al, 2019). Figure 8 shows the comparison between the mechanical properties of CS-Ba in the degradation process with other polymers and soft tissues (Ashby, 2011;Jang et al, 2018;Liravi and Toyserkani, 2018).…”

Section: Mechanical Properties Of Cs-ba Ducts and Their Matching With Soft Tissuementioning

confidence: 99%

Radiopaque Chitosan Ducts Fabricated by Extrusion-Based 3D Printing to Promote Healing After Pancreaticoenterostomy

Pan

Zhao

et al. 2021

Front. Bioeng. Biotechnol.

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Long-term placement of non-degradable silicone rubber pancreatic duct stents in the body is likely to cause inflammation and injury. Therefore, it is necessary to develop degradable and biocompatible stents to replace silicone rubber tubes as pancreatic duct stents. The purpose of our research was to verify the feasibility and biological safety of extrusion-based 3D printed radiopaque chitosan (CS) ducts for pancreaticojejunostomy. Chitosan-barium sulfate (CS-Ba) ducts with different molecular weights (low-, medium-, and high-molecular weight CS-Ba: LCS-Ba, MCS-Ba, and HCS-Ba, respectively) were soaked in vitro in simulated pancreatic juice (SPJ) (pH 8.0) with or without pancreatin for 16 weeks. Changes in their weight, water absorption rate and mechanical properties were tested regularly. The biocompatibility, degradation and radiopaque performance were verified by in vivo and in vitro experiments. The results showed that CS-Ba ducts prepared by this method had regular compact structures and good molding effects. In addition, the lower the molecular weight of the CS-Ba ducts was, the faster the degradation rate was. Extrusion-based 3D-printed CS-Ba ducts have mechanical properties that match those of soft tissue, good biocompatibility and radioopacity. In vitro studies have also shown that CS-Ba ducts can promote the growth of fibroblasts. These stents have great potential for use in pancreatic duct stent applications in the future.

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“…10,20,21 It is accordingly crucial to develop a vascular scaffold that can guide the alignment and orientation of ECs and satisfy the requirements of artificial vascular transplantation. According to recent research, 22–27 Fused Deposition Molding (FDM) has been widely used in three-dimensional printing (3D printing) forming technology, which can accurately control the diameter and arrangement of extruded fibers. Li et al.…”

Section: Introductionmentioning

confidence: 99%

“…Lee et al. 27 printed PVA mold according to medical image 3D and obtained soft thin-walled tubular scaffold by dip-coating method. Inspired by these researches, we speculated that the 3D sacrificial template was printed using PVA as the electrospinning collectors to form the oriented micro-pattern in the inner surface of vascular grafts to simulate the native structure of vascular to promote HUVECs directed growth to accelerate endothelialization.…”

Section: Introductionmentioning

confidence: 99%

An integrated strategy for designing and fabricating triple-layer vascular graft with oriented microgrooves to promote endothelialization

et al. 2021

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Compared with native blood vessels and existing vascular grafts, design and manufacture of vascular grafts with a three-dimensional topological structure is a key to induce cells and tissue growth, which remains an essential issue in both tissue engineering and regenerative medicine. This study sought to develop a novel triple-layer vascular graft (TLVG) with oriented microgrooves to investigate the mechanical property and endothelialization. The TLVGs were composed of electrospun Poly-ε-caprolactone (PCL)/thermoplastic polyurethane (TPU) as the inner layer, albumen/sodium alginate (SA) hydrogel as the middle layer, and electrospun PCL/TPU as the outer layer. In detail, a cylindrical sacrificial template was designed and printed using polyvinyl alcohol (PVA), served as the electrospinning receiving platform to form the oriented microgrooves in the inner layer of TLVGs. The highly elastic albumen/SA hydrogel and PCL/TPU nanofibers were able to simulate the elastin in blood vessels. In addition, the introduction of the albumen/SA hydrogel layer not only solves the leakage problem of a porous vascular graft but also improves the wettability of the scaffolds. The physicochemical properties and biological characteristics of TLVGs were evaluated by tensile testing, Surface wettability test, Fourier transform-infrared spectroscopy (FTIR) measurement, Live-Dead cell staining assay, and CCK-8 assay. Especially, the oriented microgrooves on the inner surface of the TLVGs can promote human umbilical vein endothelial cells (HUVECs) directed growth and migration in favor of endothelialization. All results showed that the fabricated TLVGs with excellent physicochemical properties and biocompatibility has great potential in clinic application.

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Fabrication of 3D freeform porous tubular constructs with mechanical flexibility mimicking that of soft vascular tissue

Cited by 30 publications

References 24 publications

Evaluation of 3D Templated Synthetic Vascular Graft Compared with Standard Graft in a Rat Model: Potential Use as an Artificial Vascular Graft in Cardiovascular Disease

Evaluation of 3D Templated Synthetic Vascular Graft Compared with Standard Graft in a Rat Model: Potential Use as an Artificial Vascular Graft in Cardiovascular Disease

Radiopaque Chitosan Ducts Fabricated by Extrusion-Based 3D Printing to Promote Healing After Pancreaticoenterostomy

An integrated strategy for designing and fabricating triple-layer vascular graft with oriented microgrooves to promote endothelialization

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