Fabrication of 3D scaffolds reproducing intestinal epithelium topography by high-resolution 3D stereolithography

Creff, Justine; Courson, Rémi; Mangeat, Thomas; Foncy, Julie; Souleille, Sandrine; Thibault, Christophe; Besson, Arnaud; Malaquin, Laurent

doi:10.1016/j.biomaterials.2019.119404

Cited by 118 publications

(102 citation statements)

References 58 publications

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“…After the scaffold is finished printing, it is placed under a UV light where it is postcured (Eltom et al, 2019). Creff et al (2019) fabricated a 3D model of the intestinal epithelium in vitro by combining a photopolymerizable hydrogel that promotes the growth of intestinal cell lines with stereolithography 3DP. Caco-2 intestinal epithelial cells were grown on the scaffold for 2 weeks and showed much higher rates of differentiation than standard 2D cultures (Creff et al, 2019).…”

Section: Dp Scaffolds For 3d Cell Culture Via Stereolithographymentioning

confidence: 99%

“…Creff et al (2019) fabricated a 3D model of the intestinal epithelium in vitro by combining a photopolymerizable hydrogel that promotes the growth of intestinal cell lines with stereolithography 3DP. Caco-2 intestinal epithelial cells were grown on the scaffold for 2 weeks and showed much higher rates of differentiation than standard 2D cultures (Creff et al, 2019). Thus, this model is a great candidate for studying intestinal homeostasis and regeneration mechanisms in vitro (Creff et al, 2019).…”

Section: Dp Scaffolds For 3d Cell Culture Via Stereolithographymentioning

confidence: 99%

“…Caco-2 intestinal epithelial cells were grown on the scaffold for 2 weeks and showed much higher rates of differentiation than standard 2D cultures (Creff et al, 2019). Thus, this model is a great candidate for studying intestinal homeostasis and regeneration mechanisms in vitro (Creff et al, 2019). Lee S.J.…”

Section: Dp Scaffolds For 3d Cell Culture Via Stereolithographymentioning

confidence: 99%

See 2 more Smart Citations

Is It Time to Start Transitioning From 2D to 3D Cell Culture?

Jensen

Teng

2020

Front. Mol. Biosci.

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Section: Dp Scaffolds For 3d Cell Culture Via Stereolithographymentioning

confidence: 99%

Section: Dp Scaffolds For 3d Cell Culture Via Stereolithographymentioning

confidence: 99%

Section: Dp Scaffolds For 3d Cell Culture Via Stereolithographymentioning

confidence: 99%

See 1 more Smart Citation

Is It Time to Start Transitioning From 2D to 3D Cell Culture?

Jensen

Teng

2020

Front. Mol. Biosci.

1,095

802

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“…Hydrogels can be engineered with different topographies and can thus be used to reproduce the 3D architecture of native tissues (Khademhosseini and Langer, 2007;Torras et al, 2018). In the case of the small intestine, several strategies have been proposed to fabricate villus-like hydrogels such as replica molding or stereolithography-based 3D printing (Wang et al, 2017;Creff et al, 2019). Using a different approach, we have recently developed a method to fabricate microstructured hydrogel scaffolds mimicking the small intestinal villi by using a photolithography-based microfabrication technique (Castaño et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Imaging the Cell Morphological Response to 3D Topography and Curvature in Engineered Intestinal Tissues

Altay

Tosi

García-Díaz

et al. 2020

Front. Bioeng. Biotechnol.

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While conventional cell culture methodologies have relied on flat, two-dimensional cell monolayers, three-dimensional engineered tissues are becoming increasingly popular. Often, engineered tissues can mimic the complex architecture of native tissues, leading to advancements in reproducing physiological functional properties. In particular, engineered intestinal tissues often use hydrogels to mimic villi structures. These fingerlike protrusions of a few hundred microns in height have a well-defined topography and curvature. Here, we examined the cell morphological response to these villus-like microstructures at single-cell resolution using a novel embedding method that allows for the histological processing of these delicate hydrogel structures. We demonstrated that by using photopolymerisable poly(ethylene) glycol as an embedding medium, the villus-like microstructures were successfully preserved after sectioning with vibratome or cryotome. Moreover, high-resolution imaging of these sections revealed that cell morphology, nuclei orientation, and the expression of epithelial polarization markers were spatially encoded along the vertical axis of the villus-like microstructures and that this cell morphological response was dramatically affected by the substrate curvature. These findings, which are in good agreement with the data reported for in vivo experiments on the native tissue, are likely to be the origin of more physiologically relevant barrier properties of engineered intestinal tissues when compared with standard monolayer cultures. By showcasing this example, we anticipate that the novel histological embedding procedure will have a positive impact on the study of epithelial cell behavior on three-dimensional substrates in both physiological and pathological situations.

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“…Firstly, Zhang and his colleagues (Zhang et al 2016 ) established the technique that can be transformed into human cardiomyocytes derived from iPSCs to construct endothelialized human myocardium. Then, Creff and colleagues (Creff et al 2019 ) provided the possibility of creating artificial 3D scaffolds that match the size and structure of mouse intestinal crypt and villi. Moreover, Homan and colleagues (Homan et al 2019 ) built a model that had the ability to induce substantial vascularization and morphogenesis of renal organs in vitro under flow conditions opening up a new way for the study of renal development, disease and regeneration.…”

Section: Organoids For Translational Researchmentioning

confidence: 99%

Organoid based personalized medicine: from bench to bedside

Tang

Cai

et al. 2020

Cell Regen

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Three-dimensional cultured organoids have become a powerful in vitro research tool that preserves genetic, phenotypic and behavioral trait of in vivo organs, which can be established from both pluripotent stem cells and adult stem cells. Organoids derived from adult stem cells can be established directly from diseased epithelium and matched normal tissues, and organoids can also be genetically manipulated by CRISPR-Cas9 technology. Applications of organoids in basic research involve the modeling of human development and diseases, including genetic, infectious and malignant diseases. Importantly, accumulating evidence suggests that biobanks of patient-derived organoids for many cancers and cystic fibrosis have great value for drug development and personalized medicine. In addition, organoids hold promise for regenerative medicine. In the present review, we discuss the applications of organoids in the basic and translational research.

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Fabrication of 3D scaffolds reproducing intestinal epithelium topography by high-resolution 3D stereolithography

Cited by 118 publications

References 58 publications

Is It Time to Start Transitioning From 2D to 3D Cell Culture?

Is It Time to Start Transitioning From 2D to 3D Cell Culture?

Imaging the Cell Morphological Response to 3D Topography and Curvature in Engineered Intestinal Tissues

Organoid based personalized medicine: from bench to bedside

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