Fabrication of a 3D microfluidic cell culture device for bone marrow-on-a-chip

Kefallinou, Dionysia; Grigoriou, Maria; Boumpas, Dimitrios T.; Gogolides, Εvangelos; Tserepi, Angeliki

doi:10.1016/j.mne.2020.100075

Cited by 23 publications

(9 citation statements)

References 52 publications

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“…The microchambers, coated with collagen type I, presented an organized cell expansion in stromal tissue, a well-developed 3D matrix. This figure has been adapted from a study by Tserepi et al [ 15 ].…”

Section: Figurementioning

confidence: 99%

“…The authors demonstrated a scaffold-free BMoC device as a study platform for systemic lupus erythematosus (SLE), which is illustrated in Figure 1 . The study marked a preliminary step for a complete development of perivascular system-on-chip [ 15 ]. OoAC platforms also provide the opportunity for personalized medicine as the human-induced pluripotent stem cells allow for the investigation of therapies that are tailored to specific patients or populations [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Microfluidic Organ-on-A-chip: A Guide to Biomaterial Choice and Fabrication

Cao

Zhang

Chen

et al. 2023

IJMS

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Organ-on-a-chip (OoAC) devices are miniaturized, functional, in vitro constructs that aim to recapitulate the in vivo physiology of an organ using different cell types and extracellular matrix, while maintaining the chemical and mechanical properties of the surrounding microenvironments. From an end-point perspective, the success of a microfluidic OoAC relies mainly on the type of biomaterial and the fabrication strategy employed. Certain biomaterials, such as PDMS (polydimethylsiloxane), are preferred over others due to their ease of fabrication and proven success in modelling complex organ systems. However, the inherent nature of human microtissues to respond differently to surrounding stimulations has led to the combination of biomaterials ranging from simple PDMS chips to 3D-printed polymers coated with natural and synthetic materials, including hydrogels. In addition, recent advances in 3D printing and bioprinting techniques have led to the powerful combination of utilizing these materials to develop microfluidic OoAC devices. In this narrative review, we evaluate the different materials used to fabricate microfluidic OoAC devices while outlining their pros and cons in different organ systems. A note on combining the advances made in additive manufacturing (AM) techniques for the microfabrication of these complex systems is also discussed.

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Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microfluidic Organ-on-A-chip: A Guide to Biomaterial Choice and Fabrication

Cao

Zhang

Chen

et al. 2023

IJMS

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“…Although MPS has been widely investigated, only a few published articles presently report using stem cells, including ASCs ( Zhang et al, 2017 ; Paek et al, 2019 ; O’Donnell et al, 2020 ; Marrazzo et al, 2021 ). Kefallinou and others demonstrated the generation of bone marrow-on-a-chip (BMoC), which may be useful as a system to study the stromal niche in diseases such as SLE ( Kefallinoua et al, 2020 ). Another article published by Lavrentieva described a microfluidic gradient system using hASCs and human umbilical cord vein endothelial cells (HUVECs) were embedded in a methacrylated gelatin (GelMA) hydrogel to study the morphological changes and cellular interaction in a stem cell niche and how the stiffness of the gels plays a significant role in developing scaffolds used for clinical applications ( Lavrentieva et al, 2020 ).…”

Section: Future Applications For Ascsmentioning

confidence: 99%

Adipose Stem Cells in Regenerative Medicine: Looking Forward

Al-Ghadban

Artiles

Bunnell

2022

Front. Bioeng. Biotechnol.

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Over the last decade, stem cell-based regenerative medicine has progressed to clinical testing and therapeutic applications. The applications range from infusions of autologous and allogeneic stem cells to stem cell-derived products. Adult stem cells from adipose tissue (ASCs) show significant promise in treating autoimmune and neurodegenerative diseases, vascular and metabolic diseases, bone and cartilage regeneration and wound defects. The regenerative capabilities of ASCs in vivo are primarily orchestrated by their secretome of paracrine factors and cell-matrix interactions. More recent developments are focused on creating more complex structures such as 3D organoids, tissue elements and eventually fully functional tissues and organs to replace or repair diseased or damaged tissues. The current and future applications for ASCs in regenerative medicine are discussed here.

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“…Therefore, OoC platforms can substitute for two-dimensional cell culture and animal models due to ethical concerns and insufficient reproduction of human pathophysiology [ 351 ]. During the recent decade, OoC systems have been extensively utilized to replicate physiological microenvironment of several organs such as the gut [ 352 , 353 , 354 ], heart [ 355 , 356 , 357 ], liver [ 358 , 359 , 360 ], bone [ 361 , 362 , 363 ], kidney [ 364 , 365 , 366 ], lung [ 367 , 368 , 369 ] and brain [ 370 , 371 , 372 ]. In this section, current OoC studies based on the gut, bone, liver, brain, heart, kidney, and lung will be discussed.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Biomedical Applications of Microfluidic Devices: A Review

et al. 2022

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Both passive and active microfluidic chips are used in many biomedical and chemical applications to support fluid mixing, particle manipulations, and signal detection. Passive microfluidic devices are geometry-dependent, and their uses are rather limited. Active microfluidic devices include sensors or detectors that transduce chemical, biological, and physical changes into electrical or optical signals. Also, they are transduction devices that detect biological and chemical changes in biomedical applications, and they are highly versatile microfluidic tools for disease diagnosis and organ modeling. This review provides a comprehensive overview of the significant advances that have been made in the development of microfluidics devices. We will discuss the function of microfluidic devices as micromixers or as sorters of cells and substances (e.g., microfiltration, flow or displacement, and trapping). Microfluidic devices are fabricated using a range of techniques, including molding, etching, three-dimensional printing, and nanofabrication. Their broad utility lies in the detection of diagnostic biomarkers and organ-on-chip approaches that permit disease modeling in cancer, as well as uses in neurological, cardiovascular, hepatic, and pulmonary diseases. Biosensor applications allow for point-of-care testing, using assays based on enzymes, nanozymes, antibodies, or nucleic acids (DNA or RNA). An anticipated development in the field includes the optimization of techniques for the fabrication of microfluidic devices using biocompatible materials. These developments will increase biomedical versatility, reduce diagnostic costs, and accelerate diagnosis time of microfluidics technology.

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Fabrication of a 3D microfluidic cell culture device for bone marrow-on-a-chip

Cited by 23 publications

References 52 publications

Microfluidic Organ-on-A-chip: A Guide to Biomaterial Choice and Fabrication

Microfluidic Organ-on-A-chip: A Guide to Biomaterial Choice and Fabrication

Adipose Stem Cells in Regenerative Medicine: Looking Forward

Biomedical Applications of Microfluidic Devices: A Review

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