Fabrication of a multi-layer three-dimensional scaffold with controlled porous micro-architecture for application in small intestine tissue engineering

Knight, Toyin; Basu, Joydeep; Rivera, Elias; Spencer, Thomas; Jain, Deepak; Payne, Richard

doi:10.4161/cam.24351

Cited by 26 publications

(21 citation statements)

References 34 publications

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“…The characteristic size of cells also affects their ability to migrate into scaffolds with pores of certain sizes. If pores are too small, cells encounter difficulties in penetrating the scaffold and if pores are too large, the cells cannot bridge the pores and therefore can only spread along the struts . Morphologically the rat MSCs were found to be larger than the murine osteoblasts and more prone to spreading on tissue culture plastic.…”

Section: Discussionmentioning

confidence: 99%

“…Generally, cells exhibit a preference for adhesion to scaffolds with mean pore sizes slightly larger than the cells characteristic size. To infiltrate such scaffolds, cells conventionally use a bridging mechanism whereby neighboring cells are used as support . However, if the pore size greatly exceeds the cell size, the cells can only spread along the interconnected scaffold struts, which can negatively influence cell migration and differentiation .…”

Section: Introductionmentioning

confidence: 99%

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Effect of collagen‐glycosaminoglycan scaffold pore size on matrix mineralization and cellular behavior in different cell types

Murphy

Duffy

Schindeler

et al. 2015

J Biomedical Materials Res

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We have previously examined osteoblast behavior on porous collagen-glycosaminoglycan (CG) scaffolds with a range of mean pore sizes demonstrating superior cell attachment and migration in scaffolds with the largest pores (325 μm). Scaffolds provide a framework for construct development; therefore, it is crucial to identify the optimal pore size for augmented tissue formation. Utilizing the same range of scaffolds (85 μm - 325 μm), this study aimed to examine the effects of mean pore size on subsequent osteoblast differentiation and matrix mineralization, and to understand the mechanism by which pore size influences behavior of different cell types. Consequently, primary mesenchymal stem cells (MSCs) were assessed and their behavior compared to osteoblasts. Results demonstrated that scaffolds with the largest pore size (325 μm) facilitated improved osteoblast infiltration, earlier expression of mature bone markers osteopontin (OPN) and osteocalcin (OCN), and increased mineralization. MSCs responded similarly to osteoblasts whereby cell attachment and scaffold infiltration improved with increasing pore size. However, MSCs showed reduced cell motility, proliferation, and scaffold infiltration compared to osteoblasts. This was associated with differences in the profile of integrin subunits (α2) and collagen receptors (CD44), indicating that osteoblasts have a stronger affinity for CG scaffolds compared to MSCs. In summary, these results reveal how larger pores promote improved cell infiltration, essential for construct development, however the optimal scaffold pore size can be cell type specific. As such, this study highlights a necessity to tailor both scaffold micro-architecture and cell-type when designing constructs for successful bone tissue engineering applications.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of collagen‐glycosaminoglycan scaffold pore size on matrix mineralization and cellular behavior in different cell types

Murphy

Duffy

Schindeler

et al. 2015

J Biomedical Materials Res

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“…23 This includes surface topography, microstructure, and mechanical properties, which will influence cell adhesion, proliferation, and differentiation. 24 The use of spherical microcarriers combined with suspension bioreactors is a widely used technique to expand anchorage-dependent cells. [24][25][26] The microcarriers provide a large surface area for cell adhesion and growth in a homogeneous and controlled environment.…”

Section: Introductionmentioning

confidence: 99%

A Novel Method for Differentiation of Human Mesenchymal Stem Cells into Smooth Muscle-Like Cells on Clinically Deliverable Thermally Induced Phase Separation Microspheres

Parmar

Ahmadi²,

Day³

2015

Tissue Engineering Part C: Methods

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Muscle degeneration is a prevalent disease, particularly in aging societies where it has a huge impact on quality of life and incurs colossal health costs. Suitable donor sources of smooth muscle cells are limited and minimally invasive therapeutic approaches are sought that will augment muscle volume by delivering cells to damaged or degenerated areas of muscle. For the first time, we report the use of highly porous microcarriers produced using thermally induced phase separation (TIPS) to expand and differentiate adipose-derived mesenchymal stem cells (AdMSCs) into smooth muscle-like cells in a format that requires minimal manipulation before clinical delivery. AdMSCs readily attached to the surface of TIPS microcarriers and proliferated while maintained in suspension culture for 12 days. Switching the incubation medium to a differentiation medium containing 2 ng/ mL transforming growth factor beta-1 resulted in a significant increase in both the mRNA and protein expression of cell contractile apparatus components caldesmon, calponin, and myosin heavy chains, indicative of a smooth muscle cell-like phenotype. Growth of smooth muscle cells on the surface of the microcarriers caused no change to the integrity of the polymer microspheres making them suitable for a cell-delivery vehicle. Our results indicate that TIPS microspheres provide an ideal substrate for the expansion and differentiation of AdMSCs into smooth muscle-like cells as well as a microcarrier delivery vehicle for the attached cells ready for therapeutic applications.

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“…Various techniques have been used for the fabrication of 3D scaffolds. For example, Salt leaching, Gas forming, Phase separation, 3D printing, Selective laser sintering, Stereolithography, Fused deposition modeling, Cell encapsulation, Electrospinning (Bhamidipati, Scurto, & Detamore, ; Kang, Tabata, & Ikada, ; Knight et al, ; Loh & Choong, ). Recent advances in nanotechnology and improving of nanomaterials as biocompatible and biomimetic scaffolds for cells have provided new tools for regenerative medicine (Christenson et al, ).…”

Section: Nanofibrous Fabrication Methodsmentioning

confidence: 99%

Bone tissue engineering: Adult stem cells in combination with electrospun nanofibrous scaffolds

Moradi

Golchin

Hajishafieeha

et al. 2018

Journal Cellular Physiology

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Since bone tissue lesions caused by several reasons and has global outbreak without any attentions to the modernity level of the countries. In the other hands treatment of patients with this problem faced to the several limitations, in this because the future of the bone lesions treatments is related to the future of the bone tissue engineering. This review tries to cover the most suitable stem cells and materials from either natural or synthetic sources for bone tissue engineering. These understanding points would help researchers to further uncover the application of different adult stem cell sources in electrospinning scaffolds, promotion of nanofibrous composite construct design and adult stem cell type selection to enhance cell function and bone tissue engineering, and link laboratory investigations to clinical applications.

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Fabrication of a multi-layer three-dimensional scaffold with controlled porous micro-architecture for application in small intestine tissue engineering

Cited by 26 publications

References 34 publications

Effect of collagen‐glycosaminoglycan scaffold pore size on matrix mineralization and cellular behavior in different cell types

Effect of collagen‐glycosaminoglycan scaffold pore size on matrix mineralization and cellular behavior in different cell types

A Novel Method for Differentiation of Human Mesenchymal Stem Cells into Smooth Muscle-Like Cells on Clinically Deliverable Thermally Induced Phase Separation Microspheres

Bone tissue engineering: Adult stem cells in combination with electrospun nanofibrous scaffolds

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