Fabrication of a third-generation glucose biosensor using graphene-polyethyleneimine-gold nanoparticles hybrid

Rafighi, Parvin; Tavahodi, Mojtaba; Haghighi, Behzad

doi:10.1016/j.snb.2016.03.147

Cited by 76 publications

(36 citation statements)

References 53 publications

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“…In third‐generation biosensors, the electrons involved in the redox processes are transferred directly between the enzyme or redox protein and the electrode surface to generate the response signal; no redox mediators are used oppositely to the second‐generation electrochemical biosensors . Moreover, the use of third‐generation biosensors can allow to understand the mechanisms regarding several electron transfer processes . Electrochemical reduction (on bare or modified electrodes) may be employed for NO detection studies but it is often difficult due to interference from oxygen, whose reduction happens faster than that of NO.…”

Section: Electrochemical Detection Of Nomentioning

confidence: 99%

Nitric Oxide Detection Using Electrochemical Third‐generation Biosensors – Based on Heme Proteins and Porphyrins

et al. 2018

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Nitric oxide radical (NO) is a signalling molecule involved in virtually all forms of life. Its relevance has been leading to the development of different analytical methodologies to assess the temporal and spatial fluxes of NO under the complex biological milieu. Third‐generation electrochemical biosensors are promising tools for in loco and in vivo NO quantification and, over the past years, heme proteins and porphyrins have been used in their design. Since there are some limitations with the biorecognition element directly adsorbed onto the electrode surface, nanomaterials (carbon nanotubes, gold nanoparticles, etc.) and polymers (cellulose, chitosan, nafion®, polyacrylamide, among others) have been explored to achieve high kinetics and better biosensor performance. In this review, a broad overview of the field of electrochemical third‐generation biosensors for NO electroanalysis is presented, discussing their main characteristics and aiming new outlooks and advances in this field.

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Section: Electrochemical Detection Of Nomentioning

confidence: 99%

Nitric Oxide Detection Using Electrochemical Third‐generation Biosensors – Based on Heme Proteins and Porphyrins

et al. 2018

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“…Through electrochemical reaction of glucose oxidase (GOD), oxidase is catalyzed by electrodes to give the best performance [14]. The working potential of electrodes causes oxidase to undergo electrochemical reaction, creating a set of clear anodic and reductive peaks, which suggests that the reaction between electrodes and oxidase on the test strip is stable [15]. Other types of electrochemical biosensors for the determination of blood glucose levels based on gold structures and glucose oxidase have also been investigated [16].…”

Section: Introductionmentioning

confidence: 99%

The Influence of Blood Glucose Meter Resistance Variation on the Performance of a Biosensor with a Gold-Coated Circuit Board

et al. 2019

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In this study, a novel gold-coated test strip for blood glucose measurement has been designed. Such gold-coated test strip is feasible for mass production to achieve economies of scale. Cyclic voltammetry was applied to test strips to undergo electrochemical reaction under a potential range of ±0.4 V. Glucose oxidase (GOD) was added into K3[Fe(CN)6]. When glucose oxidase undergoes electrochemical reaction, the medium, K3[Fe(CN)6], will act as an electron acceptor, causing the electrodes on the test strip to generate a pair of clear anodic and reductive peaks. The maximum of the anodic and reductive peaks can be used as reference to adjust the resistance of the blood glucose meter. The experimental results show that by adjusting the resistance of the blood glucose meter, the accuracy of blood glucose meter reading can be tuned and blood glucose reading can be stabilized. Therefore, when the resistance of the blood glucose meter is at 2.4 KΩ, the standard deviation (STD) and coefficient of variation (CV) of the test strip are lower than those of the test strips measured at resistances of 2.2 KΩ and 2.6 KΩ. It has been proved in this study that adjusting the resistance of the blood glucose meter can optimize the chemical reaction on gold-coated test strips as well as its reading. This method can also be applied to tune the accuracy of readings for test strips coated with other materials.

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“…However, adsorption can result in enzyme leaching, reducing the sensitivity of detection. Immobilization of glucose oxidase with glutaraldehyde as crosslinker proved successful for glucose sensing [1,[32][33][34]; such crosslinking provides a stable template for enzyme immobilization and prevents enzyme leaching [34][35][36][37]. A solid immobilization strategy exposes the active enzyme site to the analyte and retains biological activity, resulting in highly sensitive biosensors.…”

Section: Introductionmentioning

confidence: 99%

Cyclic Voltammetric and Electrical Impedance Spectroscopy Studies of Graphene- and Conductive Polymer-based Enzyme Electrodes

Ismail

Ramli

Abd-Wahab

et al. 2019

IJIE

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Screen-printed carbon electrodes (SPCEs), modified with graphene oxide and poly(3,4ethylenedioxythiophene):polystyrenesulfonic acid (GO-PEDOT:PSS/SPCEs), and SPCEs modified with partially reduced GO and PEDOT:PSS (prGO-PEDOT:PSS/SPCEs) were studied for electrochemical transduction. Randles-Sevcik analysis showed that the prGO-PEDOT:PSS/SPCE has a higher effective surface area of 219.3 µm 2 in comparison to the unreduced GO-PEDOT:PSS/SPCE (87.0 µm 2) and the bare SPCE (71.7 µm 2). Using electrical impedance spectroscopy (EIS), we determined that the prGO-PEDOT:PSS/SPCE has a lower charge-transfer resistance (Rct) of 163.82 Ω in comparison to the GO-PEDOT:PSS/SPCE (427.87 Ω) and the bare SPCE (13.31 kΩ). Glucose oxidase (GOx) was immobilized on all electrode types, including GO/SPCE as additional control and tested with low (0.2 mM), intermediate (0.6 mM), and high (1 mM) glucose concentrations. GOx/GO/SPCEs showed the largest change in anodic peak current (Ipa), 8.5, 7.5 and 4.9 µA for low, intermediate, and high glucose concentrations, respectively. Interestingly, GOx/prGO-PEDOT:PSS/ SPCEs have no change in both anodic and cathodic peak current, although they exhibit better redox capability, while GOx/SPCEs have very low Ipa. The results show that the high effective surface area and low charge-transfer resistance (Rct) of prGO-PEDOT:PSS/ SPCEs do not necessarily result in a sensitive glucose sensor in cases where immobilizatio n of enzymes on the material can affect electron transfer.

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Fabrication of a third-generation glucose biosensor using graphene-polyethyleneimine-gold nanoparticles hybrid

Cited by 76 publications

References 53 publications

Nitric Oxide Detection Using Electrochemical Third‐generation Biosensors – Based on Heme Proteins and Porphyrins

Nitric Oxide Detection Using Electrochemical Third‐generation Biosensors – Based on Heme Proteins and Porphyrins

The Influence of Blood Glucose Meter Resistance Variation on the Performance of a Biosensor with a Gold-Coated Circuit Board

Cyclic Voltammetric and Electrical Impedance Spectroscopy Studies of Graphene- and Conductive Polymer-based Enzyme Electrodes

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