Fabrication of alginate based microgels for drug-sustained release: In-vitro and in-vivo evaluation

Suhail, Muhammad; Li, Xinrui; Liu, Jiayu; Hsieh, Wan-Chu; Lin, Yueh‐Min; Wu, Pao‐Chu

doi:10.1016/j.ijbiomac.2021.10.054

Cited by 21 publications

(3 citation statements)

References 48 publications

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“…Comparing the XRD pattern of both unloaded and loaded hydrogel, a small change in the peaks position of the loaded hydrogels was observed due to the encapsulation of the DS, while the rest of the network of both unloaded and loaded hydrogel was the same. The PXRD of both unloaded and drug-loaded Cp/PVA-g-PAa hydrogel was almost the same [54].…”

Section: X-ray Diffraction Studiesmentioning

confidence: 80%

Preparation, In Vitro Characterization, and Cytotoxicity Evaluation of Polymeric pH-Responsive Hydrogels for Controlled Drug Release

et al. 2022

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The aim of the current investigation was based on the development of pH-responsive hydrogels of chondroitin sulfate, carbopol, and polyvinyl alcohol polymerized with acrylic acid in the presence of ammonium persulfate and ethylene glycol dimethylacrylate for controlled drug delivery. A free radical polymerization technique was used for the preparation of these pH-responsive hydrogels. The gel fraction of the prepared hydrogels was increased with the increase in the chondroitin sulfate, carbopol, polyvinyl alcohol, and acrylic acid content, while the sol-fraction was decreased. Swelling and drug release studies were performed in various pH conditions. Greater swelling and drug release were observed at high pH values (pH 4.6 and 7.4) as compared to low pH value (pH 1.2), representing the pH-responsive nature of the synthesized hydrogels. Porosity and drug loading were increased with the incorporation of high concentrations of hydrogel contents except polyvinyl alcohol, which showed reverse effects. Similarly, biodegradation study reported a slow degradation rate of the prepared hydrogels with the increase in hydrogel constituents. Cytotoxicity study proved the safe use of developed hydrogels as no toxic effect was shown on T84 human colon cancer cells. Similarly, various characterizations, including Fourier transform infrared spectroscopy, thermogravimetric analysis, differential scanning calorimetry, X-ray diffraction, and scanning electron microscopy, were performed for prepared hydrogels. Hence, we could demonstrate that the prepared hydrogels can be used as a promising drug carrier for the controlled delivery of drugs.

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Section: X-ray Diffraction Studiesmentioning

confidence: 80%

Preparation, In Vitro Characterization, and Cytotoxicity Evaluation of Polymeric pH-Responsive Hydrogels for Controlled Drug Release

et al. 2022

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“…Drug carrier systems are being continuously engineered to carry drugs throughout the body, enhancing their safety and efficiency [ 43 , 44 , 45 , 46 ]. Among these designed systems, there are micro/meso/macroporous and super-porous structures, as well as various sizes of hydrogel such as nanogel [ 47 , 48 , 49 ], microgel [ 50 , 51 , 52 , 53 ], and cryogel [ 54 , 55 , 56 , 57 ]. In addition, polymeric structures prepared from synthetic and natural resources are also included in these systems.…”

Section: Resultsmentioning

confidence: 99%

Super-Macroporous Pulluan Cryogels as Controlled Active Delivery Systems with Controlled Degradability

et al. 2023

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Here, super-macroporous cryogel from a natural polysaccharide, pullulan was synthesized using a cryo-crosslinking technique with divinyl sulfone (DVS) as a crosslinker. The hydrolytic degradation of the pullulan cryogel in various simulated body fluids (pH 1.0, 7.4, and 9.0 buffer solutions) was evaluated. It was observed that the pullulan cryogel degradation was much faster in the pH 9 buffer solution than the pH 1.0 and 7.4 buffer solutions in the same time period. The weight loss of the pullulan cryogel at pH 9.0 within 28 days was determined as 31% ± 2%. To demonstrate the controllable drug delivery potential of pullulan cryogels via degradation, an antibiotic, ciprofloxacin, was loaded into pullulan cryogels (pullulan-cipro), and the loading amount of drug was calculated as 105.40 ± 2.6 µg/mg. The release of ciprofloxacin from the pullulan-cipro cryogel was investigated in vitro at 37.5 °C in physiological conditions (pH 7.4). The amount of drug released within 24 h was determined as 39.26 ± 3.78 µg/mg, which is equal to 41.38% ± 3.58% of the loaded drug. Only 0.1 mg of pullulan-cipro cryogel was found to inhibit half of the growing Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) colonies for 10 min and totally eradicated within 2 h by the release of the loaded antibiotic. No significant toxicity was determined on L929 fibroblast cells for 0.1 mg drug-loaded pullulan cryogel. In contrast, even 1 mg of drug-loaded pullulan cryogel revealed slight toxicity (e.g., 66% ± 9% cell viability) because of the high concentration of released drug.

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“…Al is a polysaccharide polymer and has various applications in food, agriculture, drug delivery, bone healing, cosmetics, paper, pharmaceutical and biomedical industries, etc., because it has the same structural resemblance to the extracellular matrix. The main benefit of Al is its ease of gelation in a warm environment [ 14 ]. Aa is a synthetic monomer, used broadly in both biomedical and pharmaceutical fields.…”

Section: Introductionmentioning

confidence: 99%

Preparation of pH-Responsive Hydrogels Based on Chondroitin Sulfate/Alginate for Oral Drug Delivery

Suhail

Ullah

et al. 2022

Pharmaceutics

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This study investigates pH-sensitive hydrogels based on biocompatible, biodegradable polysaccharides and natural polymers such as chondroitin sulfate and alginate in combination with synthetic monomer such as acrylic acid, as controlled drug carriers. Investigations were conducted for chondroitin sulfate/alginate-graft-poly(acrylic acid) hydrogel in various mixing ratios of chondroitin sulfate, alginate and acrylic acid in the presence of ammonium persulfate and N′,N′-Methylene bisacrylamide. Crosslinking and loading of drug were confirmed by Fourier transform infrared spectroscopy. Thermal stability of both polymers was enhanced after crosslinking as indicated by thermogravimetric analysis and differential scanning calorimeter thermogram of developed hydrogel. Similarly, surface morphology was evaluated by scanning electron microscopy, whereas crystallinity of the polymers and developed hydrogel was investigated by powder X-ray diffraction. Furthermore, swelling and drug-release studies were investigated in acidic and basic medium of pH 1.2 and 7.4 at 37 °C, respectively. Maximum swelling and drug release were detected at pH 7.4 as compared to pH 1.2. Increased incorporation of hydrogel contents led to an increase in porosity, drug loading, and gel fraction while a reduction in sol fraction was seen. The polymer volume fraction was found to be low at pH 7.4 compared to pH 1.2, indicating a prominent and greater swelling of the prepared hydrogels at pH 7.4. Likewise, a biodegradation study revealed a slow degradation rate of the developed hydrogel. Hence, we can conclude from the results that a fabricated system of hydrogel could be used as a suitable carrier for the controlled delivery of ketorolac tromethamine.

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Fabrication of alginate based microgels for drug-sustained release: In-vitro and in-vivo evaluation

Cited by 21 publications

References 48 publications

Preparation, In Vitro Characterization, and Cytotoxicity Evaluation of Polymeric pH-Responsive Hydrogels for Controlled Drug Release

Preparation, In Vitro Characterization, and Cytotoxicity Evaluation of Polymeric pH-Responsive Hydrogels for Controlled Drug Release

Super-Macroporous Pulluan Cryogels as Controlled Active Delivery Systems with Controlled Degradability

Preparation of pH-Responsive Hydrogels Based on Chondroitin Sulfate/Alginate for Oral Drug Delivery

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