Fabrication of alginate-based stimuli-responsive, non-cytotoxic, terpolymric semi-IPN hydrogel as a carrier for controlled release of bovine albumin serum and 5-amino salicylic acid

Das, Dipankar; Pham, Hien Thi Thu; Lee, Seongho; Noh, Insup

doi:10.1016/j.msec.2018.12.127

Cited by 54 publications

(20 citation statements)

References 44 publications

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“…Sodium alginate (Alg) is a natural polysaccharide with β- d -mannuronic acid and α- l -guluronic acid repeating units [ 28 ], which shows the typical advantages of polysaccharides for biomedical applications, together with a high degradation rate allowing applications such as cell encapsulating and wound healing devices [ 29 , 30 , 31 ], as well as a coadjuvant in protein crystallization [ 32 , 33 ].…”

Section: Resultsmentioning

confidence: 99%

Alginate Bioconjugate and Graphene Oxide in Multifunctional Hydrogels for Versatile Biomedical Applications

et al. 2021

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In this work, we combined electrically-conductive graphene oxide and a sodium alginate-caffeic acid conjugate, acting as a functional element, in an acrylate hydrogel network to obtain multifunctional materials designed to perform multiple tasks in biomedical research. The hybrid material was found to be well tolerated by human fibroblast lung cells (MRC-5) (viability higher than 94%) and able to modify its swelling properties upon application of an external electric field. Release experiments performed using lysozyme as the model drug, showed a pH and electro-responsive behavior, with higher release amounts and rated in physiological vs. acidic pH. Finally, the retainment of the antioxidant properties of caffeic acid upon conjugation and polymerization processes (Trolox equivalent antioxidant capacity values of 1.77 and 1.48, respectively) was used to quench the effect of hydrogen peroxide in a hydrogel-assisted lysozyme crystallization procedure.

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Section: Resultsmentioning

confidence: 99%

Alginate Bioconjugate and Graphene Oxide in Multifunctional Hydrogels for Versatile Biomedical Applications

et al. 2021

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“…As for the culture conditions, studies on the proliferation and characteristics of the cells according to the biological and physical culture conditions may be carried out such as the components of the culture medium, the continuous supply of new micro-carrier, and the stirring speed. Mass production of diverse cells like mesenchymal stems, keratinocytes and chondrocytes [25][26][27] is expected to be employed as sources for tissue engineering of bone, cartilage, blood vessels and skins by using diverse recent technologies such as 3D bioprinting [28,29], microchips, hydrogels and others [30][31][32].…”

Section: Discussionmentioning

confidence: 99%

Tissue Regeneration of Human Mesenchymal Stem Cells on Porous Gelatin Micro-Carriers by Long-Term Dynamic In Vitro Culture

Nguyen

Bang

Noh

2019

Tissue Eng Regen Med

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BACKGROUND: Tissue engineering is a multidisciplinary field which attracted much attention in recent years. One of the most important issue in tissue engineering is how to obtain high cell numbers and tissue regeneration while maintaining appropriate cellular characteristics in vitro for restoring damaged or dysfunctional body tissues and organs. These demands can be achieved by the use of three dimensional (3D) dynamic cultures of cells combined with cell-adhesive micro-carriers. METHOD: In this study, human mesenchymal stem cells (hMSCs) were cultured in a wave-bioreactor system for up to 100 days, after seeding on Cultisphere-S porous gelatin micro-carriers. Cell counting was performed at the time points of 7, 12, 17, 31 days and compared to those of hMSCs cultured under static condition. Higher growth and proliferation rates was achieved in wave-type dynamic culture, when cell culture continued to day 31. A scanning electron microscope (SEM) photographs, both live and dead and MTT assays were taken to confirm the survival and distribution of cells on porous gelatin micro-carrier surfaces. The results of histological stains such as hematoxylin and eosin, Masson's trichrome, Alcian blue and Alizarin red S also showed improved proliferation and tissue regeneration of hMSCs on porous gelatin micro-carriers. CONCLUSION: The experimental results demonstrated the effect and importance of both micro-carriers and bioreactor in hMSC expansion on cell proliferation and migration as well as extracellular matrix formation on the superficial and pore surfaces of the porous gelatin micro-carriers, and then their inter-connections, leading to tissue regeneration.

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“…They would be promising in biomaterial applications. Das et al (2019) developed a semi-IPN hydrogel with improved mechanical properties by cross-linking alginate with synthetic polymer via free radical polymerization. It is a potential application for drug delivery vehicle.…”

Section: Classification Of Hydrogelsmentioning

confidence: 99%

Rational Design of Smart Hydrogels for Biomedical Applications

Zhang

Huang

2021

Front. Chem.

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Hydrogels are polymeric three-dimensional network structures with high water content. Due to their superior biocompatibility and low toxicity, hydrogels play a significant role in the biomedical fields. Hydrogels are categorized by the composition from natural polymers to synthetic polymers. To meet the complicated situation in the biomedical applications, suitable host–guest supramolecular interactions are rationally selected. This review will have an introduction of hydrogel classification based on the formulation molecules, and then a discussion over the rational design of the intelligent hydrogel to the environmental stimuli such as temperature, irradiation, pH, and targeted biomolecules. Further, the applications of rationally designed smart hydrogels in the biomedical field will be presented, such as tissue repair, drug delivery, and cancer therapy. Finally, the perspectives and the challenges of smart hydrogels will be outlined.

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Fabrication of alginate-based stimuli-responsive, non-cytotoxic, terpolymric semi-IPN hydrogel as a carrier for controlled release of bovine albumin serum and 5-amino salicylic acid

Cited by 54 publications

References 44 publications

Alginate Bioconjugate and Graphene Oxide in Multifunctional Hydrogels for Versatile Biomedical Applications

Alginate Bioconjugate and Graphene Oxide in Multifunctional Hydrogels for Versatile Biomedical Applications

Tissue Regeneration of Human Mesenchymal Stem Cells on Porous Gelatin Micro-Carriers by Long-Term Dynamic In Vitro Culture

Rational Design of Smart Hydrogels for Biomedical Applications

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