Fabrication of CdTe@SiO2 nanoprobes for sensitive electrogenerated chemiluminescence detection of DNA damage

Wei, Wei; Zhou, Jie; Li, Henan; Yin, Lihong; Pu, Yuepu; Liu, Songqin

doi:10.1039/c3an00264k

Cited by 21 publications

(10 citation statements)

References 33 publications

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“…16,17,44,45 Many biological methods, including Ames assay, comet assay, DNA fragmentation test, have been used for the evaluation of genotoxicity, 44,45 along with some novel approaches. [46][47][48] Flow cytometry analysis has also been used as a powerful tool for the detection of DNA damage in cells. [49][50][51] To investigate the possible DNA damage caused by GQDs, we monitored the expression levels of p53 and Rad 51 proteins in response to the GQD treatment by staining the cells with the respective fluorochromes, followed by flow cytometry analysis.…”

Section: Resultsmentioning

confidence: 99%

Can graphene quantum dots cause DNA damage in cells?

et al. 2015

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Graphene quantum dots (GQDs) have attracted tremendous attention for biological applications. We report the first study on cytotoxicity and genotoxicity of GQDs to fibroblast cell lines (NIH-3T3 cells). The NIH-3T3 cells treated with GQDs at dosages over 50 μg mL(-1) showed no significant cytotoxicity. However, the GQD-treated NIH-3T3 cells exhibited an increased expression of proteins (p53, Rad 51, and OGG1) related to DNA damage compared with untreated cells, indicating the DNA damage caused by GQDs. The GQD-induced release of reactive oxygen species (ROS) was demonstrated to be responsible for the observed DNA damage. These findings should have important implications for future applications of GQDs in biological systems.

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Section: Resultsmentioning

confidence: 99%

Can graphene quantum dots cause DNA damage in cells?

et al. 2015

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“…E-learning has the potential to foster or reinforce new training strategies around the learner, often designated as “a learner-centered pedagogy”: i.e., a pedagogy based on motivation, information, analysis, interaction and production [1]. On the other hand, specific attention should be given to the “importance of curriculum design for learning performance” [36].…”

Section: Discussionmentioning

confidence: 99%

“…The integration and the assimilation of ever changing knowledge, the management of an increasing number of learners and the gradual transition from a “traditional” model (i.e. ex cathedra lectures) to an “active” and “interactive” model where the learner is the main actor of his learning process [1,2]. In this perspective, the use of ICT, including Internet, represents a promising approach.…”

Section: Introductionmentioning

confidence: 99%

Computer literacy and E-learning perception in Cameroon: the case of Yaounde Faculty of Medicine and Biomedical Sciences

et al. 2013

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BackgroundHealth science education faces numerous challenges: assimilation of knowledge, management of increasing numbers of learners or changes in educational models and methodologies. With the emergence of e-learning, the use of information and communication technologies (ICT) and Internet to improve teaching and learning in health science training institutions has become a crucial issue for low and middle income countries, including sub-Saharan Africa. In this perspective, the Faculty of Medicine and Biomedical Sciences (FMBS) of Yaoundé has played a pioneering role in Cameroon in making significant efforts to improve students’ and lecturers’ access to computers and to Internet on its campus.The objective is to investigate how computer literacy and the perception towards e-learning and its potential could contribute to the learning and teaching process within the FMBS academic community.MethodA cross-sectional survey was carried out among students, residents and lecturers. The data was gathered through a written questionnaire distributed at FMBS campus and analysed with routine statistical software.Results307 participants answered the questionnaire: 218 students, 57 residents and 32 lecturers. Results show that most students, residents and lecturers have access to computers and Internet, although students’ access is mainly at home for computers and at cyber cafés for Internet. Most of the participants have a fairly good mastery of ICT. However, some basic rules of good practices concerning the use of ICT in the health domain were still not well known. Google is the most frequently used engine to retrieve health literature for all participants; only 7% of students and 16% of residents have heard about Medical Subject Headings (MeSH).The potential of e-learning in the improvement of teaching and learning still remains insufficiently exploited. About two thirds of the students are not familiar with the concept of e-leaning. 84% of students and 58% of residents had never had access to e-learning resources. However, most of the participants perceive the potential of e-learning for learning and teaching, and are in favour of its development at the FMBS.ConclusionThe strong interest revealed by the study participants to adopt and follow-up the development of e-learning, opens new perspectives to a faculty like the FMBS, located in a country with limited resources. However, the success of its development will depend on different factors: the definition of an e-learning strategy, the implementation of concrete measures and the adoption of a more active and participative pedagogy.

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“…22 Compared with conventional molecular luminophores, quantum dots (QDs) possess several unique merits like unique optical, electronic, and electrochemical characteristics. 23 QD based ECL has been widely applied in biosensing and immunoassays.…”

Section: Introductionmentioning

confidence: 99%

Dual-signal amplified electrochemiluminescence immunoassay for salbutamol based on quantum dots and gold nanoparticle-labeled horseradish peroxidase

Cai

Wang

Dong

et al. 2015

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This study describes a novel electrochemical immunosensor to amplify the electrochemiluminescence (ECL) signal for the ultrasensitive detection of salbutamol (SAL) using quantum dots (QDs) and gold nanoparticle (AuNP) conjugated horseradish peroxidase (HRP). The electrochemical detection was based on the HRP catalyzed consumption of self-produced H2O2, which has been extensively used as a co-reactant of QDs, by o-phenylenediamine (OPD). The enzymatic reaction rate is proportional to the amount of HRP bound to the electrode. In the presence of a SAL standard solution, the immobilized SAL coating antigens competed with the SAL solution for the Ab-AuNPs-HRP complexes. With an increase in the SAL concentration, the amount of immobilized HRP decreases, which leads to an increase in the ECL intensity. Under optimized conditions, the ECL intensity changes linearly with the logarithm of the SAL concentration in the range of 0.05-500 ng mL(-1) with a detection limit of 0.017 ng mL(-1) (S/N = 3). The ECL immunosensor possesses high sensitivity, satisfactory reproducibility and selectivity, and may provide a feasible route for practical application.

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Fabrication of CdTe@SiO2 nanoprobes for sensitive electrogenerated chemiluminescence detection of DNA damage

Cited by 21 publications

References 33 publications

Can graphene quantum dots cause DNA damage in cells?

Can graphene quantum dots cause DNA damage in cells?

Computer literacy and E-learning perception in Cameroon: the case of Yaounde Faculty of Medicine and Biomedical Sciences

Dual-signal amplified electrochemiluminescence immunoassay for salbutamol based on quantum dots and gold nanoparticle-labeled horseradish peroxidase

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