Fabrication of chitosan/graphene oxide polymer nanofiber and its biocompatibility for cartilage tissue engineering

Cao, Lei; Zhang, Fei; Wang, Qiugen; Wu, Xiaofeng

doi:10.1016/j.msec.2017.05.056

Cited by 120 publications

(72 citation statements)

References 25 publications

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“…In addition, once functionalized with biomolecules like polysaccharides [41], proteins [42], etc. or other biological systems graphene can be integrated for developing new applications in biomedicine and bio-nanotechnology such as biosensors [43], biocatalysis [44], biofuel cells [45], etc.…”

Section: Graphene and Graphene Oxidementioning

confidence: 99%

Graphene Nanocomposites Studied by Raman Spectroscopy

Bîru¹,

Iovu²

2018

Raman Spectroscopy

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The goal of this chapter is to provide a general introduction about graphene nanocomposites studied by Raman spectroscopy. The chapter will therefore begin with a brief description of the major Raman bands of carbon allotropes. In the following chapter a concise comparison between single walled carbon nanotubes (SWCNTs), multi-walled carbon nanotubes (MWCNTs), fullerenes and graphene is exposed. The characteristic features in Raman spectra of carbon allotropes, namely the intense signals D and G are investigated. In particular, the chapter will outline the Raman spectrum of graphene and different types of graphene oxide. The last part of the chapter is devoted to graphene nanocomposites.

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Section: Graphene and Graphene Oxidementioning

confidence: 99%

Graphene Nanocomposites Studied by Raman Spectroscopy

Bîru¹,

Iovu²

2018

Raman Spectroscopy

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“…[24] Graphene oxide (GO) has been shown to support the growth of the 3T3 rabbit cartilage cell [9] line as well as ATDC5 chondroprogenitor cells. [51] GO has been used specifically to add mechanical robustness to porous hydrogels used for cartilage tissue engineering. While GO is effective as a mechanical strengthening mechanism in hydrogels, GO does not exhibit high electrical conductivity as it is an electrical insulator.…”

Section: Resultsmentioning

confidence: 99%

Mechanical Properties of Graphene Foam and Graphene Foam—Tissue Composites

et al. 2018

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Graphene foam (GF), a 3-dimensional derivative of graphene, has received much attention recently for applications in tissue engineering due to its unique mechanical, electrical, and thermal properties. Although GF is an appealing material for cartilage tissue engineering, the mechanical properties of GF – tissue composites under dynamic compressive loads have not yet been reported. The objective of this study was to measure the elastic and viscoelastic properties of GF and GF-tissue composites under unconfined compression when quasi-static and dynamic loads are applied at strain magnitudes below 20%. The mechanical tests demonstrate a 46% increase in the elastic modulus and a 29% increase in the equilibrium modulus after 28-days of cell culture as compared to GF soaked in tissue culture medium for 24h. There was no significant difference in the amount of stress relaxation, however, the phase shift demonstrated a significant increase between pure GF and GF that had been soaked in tissue culture medium for 24h. Furthermore, we have shown that ATDC5 chondrocyte progenitor cells are viable on graphene foam and have identified the cellular contribution to the mechanical strength and viscoelastic properties of GF – tissue composites, with important implications for cartilage tissue engineering.

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“…The CPL drug showed two decomposition dips at 200 C, which could be attributed to beta-lactam ring and complete decomposition of CPL drug at 400 C. It was hence seen that the conjugates show similar losses in their weights, which are commensurate with the losses as observed in their basic constituents, that is, CPL and CS-GAPC MNs. However, because both CS and GAPC show fairly good thermal stability, [48][49][50][51][52][53][54][55][56][57][58][59][60][61] it was difficult to evaluate the loading.…”

Section: Ftir Analysismentioning

confidence: 99%

Microneedles of chitosan‐porous carbon nanocomposites: Stimuli (pH and electric field)‐initiated drug delivery and toxicological studies

Gaware

Rokade

Bala

et al. 2019

J Biomedical Materials Res

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An array of microneedles (MNs) of chitosan‐graphene assembled in porous carbon (CS‐GAPC) nanocomposites has been synthesized and evaluated. The safety of the formulated system has been ensured using detailed in vivo toxicological studies and efficacy has been ensured by evaluating the stimuli (pH and electric field) initiated drug delivery properties. Drug cephalexin has been incorporated in these MNs. In vivo toxicological studies of CS‐GAPC nanocomposite were performed on Sprague rats, using acute dermal and subacute dermal (ADT& SADT) test, histopathological studies, biochemical studies, and AMES tests. ADT and SADT studies showed that median lethal dose (LD50) was found greater than 2000 mg/kg body weight; with no abnormal weight gain and food consumption, during the study period of 28 days. This study showed that administration of CS‐GAPC did not cause any substantial alterations in hematological and biochemical parameters of the animals. Histopathological studies showed no significant changes in the control and CS‐GAPC administered groups. AMES tests reveal that CS‐GAPC nanocomposite is nonmutagenic against the Salmonella thyphimurium strains. No abnormalities were observed in the animal's chromosomal aberrations and clastogenic values when the animals were treated with CS‐GAPC. At acidic pH of 4, the encapsulated drug was completely released, indicating that the drug release from the prepared nanocomposite is pH dependent. An electric field of 5 V showed optimum drug release, as a function of applied electric pulses. A biologically safe drug encapsulation model system is hence projected for smart drug delivery (pH dependent and electric field triggered) using the microneedle approach. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1582–1596, 2019.

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Fabrication of chitosan/graphene oxide polymer nanofiber and its biocompatibility for cartilage tissue engineering

Cited by 120 publications

References 25 publications

Graphene Nanocomposites Studied by Raman Spectroscopy

Graphene Nanocomposites Studied by Raman Spectroscopy

Mechanical Properties of Graphene Foam and Graphene Foam—Tissue Composites

Microneedles of chitosan‐porous carbon nanocomposites: Stimuli (pH and electric field)‐initiated drug delivery and toxicological studies

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