Fabrication of colloidal silver-peptide nanocomposites for bacterial wound healing

Ovais, Muhammad; Ali, Arbab; Ullah, Sana; Khalil, Ali Talha; Atiq, Atia; Atiq, Maria; Doǧan, N.; Shinwari, Zabta Khan; Abbas, Manzar

doi:10.1016/j.colsurfa.2022.129708

Cited by 19 publications

(12 citation statements)

References 33 publications

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“…From the CAC plot shown in Figure S12 , it appears to us that among all, LVK-PA possesses the smallest value of CAC (63 μM), which is quite expected from the presence of both “FF” and “WW” residues within its core structure. The π–π interaction among the “FF” and “WW” residues expedites the self-assembly process. ,, In this context, it is important to note that the presence of “FF” moiety on the “LVFFA” domain plays a crucial role in its aggregation property. − Both AIK-PA (97.7 μM) and VGK-PA (100.57 μM) show comparatively higher CAC and a lower rate of self-assembly than LVK-PA at a given concentration; again, the lesser hydrophobicity of the core amphiphile skeleton could be the reason. Solvatochromic dye Nile red being extremely sensitive toward the polar environment shows higher fluorescence upon its entrapment within self-assembled nanostructures containing a hydrophobic core.…”

Section: Resultsmentioning

confidence: 99%

“…The successful genesis of an engineered AMP-based biomaterial requires a fine balance between amphiphilic property in combination with its cationic nature and ability to acquire secondary structures so that it must not exert cytotoxicity to the mammalian cells. , Earlier Reches and Gazit identified “FF” dipeptide from the core hydrophobic region of amyloid beta 42 which established a broad application avenue of bioinspired short peptide-based material with multifaceted use. , Extrapolating its use Schnaider et al reported the use of “FF” dipeptide as the most minimalistic model of self-assembling peptide-based antimicrobial nanostructures . Following this, Ovais et al in their work used “FFssFF” as a capping agent for the formation of colloidal Ag/FFssFF composite nanoparticles having significant activity against both Gram-positive and Gram-negative bacterial strains . Shreds of evidence suggest that various hydrophobic beta aggregation-prone peptides similar to “FF” in combination with metal ions like Ag + , and Zn 2+ assembly can give rise to nanomaterials with improved antimicrobial and anti-biofilm effectivity. , However, the emergence of multidrug resistance bacteria, unavailability of biochemical cues, compromised polarity, and biocompatibility restricted their use as advanced tropical antibacterial wound healing therapeutics.…”

Section: Introductionmentioning

confidence: 99%

“…19 Following this, Ovais et al in their work used "FFssFF" as a capping agent for the formation of colloidal Ag/FFssFF composite nanoparticles having significant activity against both Gram-positive and Gramnegative bacterial strains. 20 Shreds of evidence suggest that various hydrophobic beta aggregation-prone peptides similar to "FF" in combination with metal ions like Ag + , and Zn 2+ assembly can give rise to nanomaterials with improved antimicrobial and anti-biofilm effectivity. 21,22 However, the emergence of multidrug resistance bacteria, unavailability of biochemical cues, compromised polarity, and biocompatibility restricted their use as advanced tropical antibacterial wound healing therapeutics.…”

Section: Introductionmentioning

confidence: 99%

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Amyloid-Inspired Engineered Multidomain Amphiphilic Injectable Peptide Hydrogel─An Excellent Antibacterial, Angiogenic, and Biocompatible Wound Healing Material

Mukherjee

Ghosh

Sarkar

et al. 2023

ACS Appl. Mater. Interfaces

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The ingrained mechanical robustness of amyloids in association with their fine-tunable physicochemical properties results in the rational design and synthesis of tailor-made biomaterials for specific applications. However, the incredible antimicrobial efficacy of these ensembles has largely been overlooked. This research work provides an insight into the interplay between selfassembly and antimicrobial activity of amyloid-derived peptide amphiphiles and thereby establishes a newfangled design principle toward the development of potent antimicrobial materials with superior wound healing efficacy. Apart from the relationship with many neurodegenerative diseases, amyloids are now considered as an important cornerstone of our innate immune response against pathogenic microbes. Impelled by this observation, a class of amphiphilic antimicrobial peptide-based biomaterial has been designed by taking Aβ42 as a template. The designed AMP due to its amphipathic nature undergoes rapid self-assembly to form a biocompatible supramolecular hydrogel network having significant antibacterial as well as wound healing effectivity on both Gramnegative P. aeruginosa and MRSA-infected diabetic wounds via reduced inflammatory response and enhanced angiogenesis. Results suggest that disease-forming amyloids can be used as a blueprint for the fabrication of biomaterial-based antimicrobial therapeutics by fine-tuning both the hydrophobicity of the β-aggregation prone zone as well as membrane interacting cationic residues.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Amyloid-Inspired Engineered Multidomain Amphiphilic Injectable Peptide Hydrogel─An Excellent Antibacterial, Angiogenic, and Biocompatible Wound Healing Material

Mukherjee

Ghosh

Sarkar

et al. 2023

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…Herein, we observed inflammatory reactions and intestinal microbiota alterations after long-term sublethal exposure to Ag NPs. Based on the physicochemical properties, the strong biological effects of Ag NPs would be mainly because of their small size and excellent colloidal stability (referring to the capping) . In addition, Ag NPs could be uptaken by DCs and induced abnormal maturation through ROS production.…”

Section: Discussionmentioning

confidence: 99%

“…Based on the physicochemical properties, the strong biological effects of Ag NPs would be mainly because of their small size and excellent colloidal stability (referring to the capping). 65 In addition, Ag NPs could be uptaken by DCs and induced abnormal maturation through ROS production. Instead of inducing cell death of DCs, the abnormal maturation of DCs activated the Bim signaling pathway and led to apoptosis.…”

Section: Phagocytosis Of Ag Nps By Dcs Led To Abnormal Activation Of ...mentioning

confidence: 99%

Intestinal Toxicity of Metal Nanoparticles: Silver Nanoparticles Disorder the Intestinal Immune Microenvironment

Ren

et al. 2023

ACS Appl. Mater. Interfaces

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Albeit numerous studies have been conducted on the toxicity evaluation of engineered metal nanoparticles (NPs), significant knowledge gaps remain regarding the influence of oral exposure to metal NPs on the intestine system, especially the effects on the intestinal immune microenvironment. Herein, we examined the long-term effects of representative engineered metal NPs on the intestine through oral exposure and identified silver NPs (Ag NPs) that resulted in severe damage. Oral Ag NP exposure damaged the epithelial structure, reduced the thickness of the mucosal layer, and altered the intestinal microbiota. Particularly, the reduced thickness of the mucosal layer increased the phagocytosis of Ag NPs by dendritic cells (DCs). Comprehensive animal and in vitro experiments unraveled that Ag NPs directly interacted with DCs, resulting in the abnormal activation of DCs by generating reactive oxygen species and inducing uncontrolled apoptosis. Furthermore, our data unveiled that the interactions between Ag NPs and DCs reduced the proportion of CD103 + CD11b + DCs and induced Th17 cell activation with inhibition of regulatory T-cell differentiation, resulting in the disordered immune microenvironment in the intestine. Collectively, these results represent a new point of view on the cytotoxicity of Ag NPs on the intestine system. This study provides additional insights into the health risks of engineered metal NPs, especially Ag NPs.

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Metal‐coordinated amino acid/peptide/protein‐based supramolecular self‐assembled nanomaterials for anticancer applications

Shabbir,

Atiq,

Wang

et al. 2024

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Biomolecules with metals can form supramolecular nanomaterials through coordination assembly, opening new avenues for cancer theranostics and bringing unique insights into personalized nanomedicine. These biomaterials have been considered versatile and innovative nanoagents due to their structure‒function control, biological nature, and simple preparation methods. This review article summarized the recent developments in multicomponent nanomaterials formed from metal coordination interactions with amino acids, peptides, and proteins, together with anticancer drugs, for cancer theranostics. We discussed the role of functional groups anchored in building blocks for coordination interactions, and subsequently, the types of interactions were examined from a structure‒function perspective. Amino acids with different metals and anticancer drugs forming supramolecular nanomaterials and their anticancer mechanisms were highlighted. Peptides with different metals and anticancer drugs, proteins with metals and anticancer drugs used for material formations, and anticancer activity have been discussed. Ultimately, the conclusion and future outlook for multicomponent supramolecular nanomaterials offer fundamental insights into fabrication design and precision medicine.

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Fabrication of colloidal silver-peptide nanocomposites for bacterial wound healing

Cited by 19 publications

References 33 publications

Amyloid-Inspired Engineered Multidomain Amphiphilic Injectable Peptide Hydrogel─An Excellent Antibacterial, Angiogenic, and Biocompatible Wound Healing Material

Amyloid-Inspired Engineered Multidomain Amphiphilic Injectable Peptide Hydrogel─An Excellent Antibacterial, Angiogenic, and Biocompatible Wound Healing Material

Intestinal Toxicity of Metal Nanoparticles: Silver Nanoparticles Disorder the Intestinal Immune Microenvironment

Metal‐coordinated amino acid/peptide/protein‐based supramolecular self‐assembled nanomaterials for anticancer applications

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