Fabrication of complex PDMS microfluidic structures and embedded functional substrates by one-step injection moulding

Szydzik, Crispin; Niego, Be׳eri; Dalzell, Geordie; Knoerzer, Markus; Ball, Fabian; Nesbitt, Warwick Scott; Medcalf, Robert L.; Khoshmanesh, Khashayar; Mitchell, Arnan

doi:10.1039/c6ra20688c

Cited by 32 publications

(25 citation statements)

References 41 publications

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“…In recent years, with the development of micro-injection molding technology, researchers began to try to use this injection molding method to process microfluidic chips. The common injection materials used in microfluidic chips are PMMA, COC and PDMS [64]. Traditionally, processing microfluidic chips by injection molding requires processing molds first, which is expensive and time-consuming.…”

Section: Injection Moldingmentioning

confidence: 99%

Application of Microfluidic Chip Technology in Food Safety Sensing

Gao

Yan

et al. 2020

Sensors

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Food safety analysis is an important procedure to control food contamination and supervision. It is urgently needed to construct effective methods for on-site, fast, accurate and popular food safety sensing. Among them, microfluidic chip technology exhibits distinguish advantages in detection, including less sample consumption, fast detection, simple operation, multi-functional integration, small size, multiplex detection and portability. In this review, we introduce the classification, material, processing and application of the microfluidic chip in food safety sensing, in order to provide a good guide for food safety monitoring.

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Section: Injection Moldingmentioning

confidence: 99%

Application of Microfluidic Chip Technology in Food Safety Sensing

Gao

Yan

et al. 2020

Sensors

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“…214,215 While complex microfluidic systems have been demonstrated with a high degree of sophistication within these areas of application, little evidence exists in the literature of development of these high throughput systems with a specific focus on manipulation of whole blood. While it is possible that this delayed application is due to fabrication impracticalities, 216 challenges associated with hemocompatibility could also play a role, and it is possible that achieving the full potential that complex on-chip microfluidic systems have demonstrated in other application areas may be dependent on careful consideration of material and operational hemocompatibility.…”

Section: Microfluidic Technologies For On-chip Blood Manipulationmentioning

confidence: 99%

A Review of Design Considerations for Hemocompatibility within Microfluidic Systems

Szydzik

Brazilek

Nesbitt

2020

Semin Thromb Hemost

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The manipulation of blood within in vitro environments presents a persistent challenge, due to the highly reactive nature of blood, and its multifaceted response to material contact, changes in environmental conditions, and stimulation during handling. Microfluidic Lab-on-Chip systems offer the promise of robust point-of-care diagnostic tools and sophisticated research platforms. The capacity for precise control of environmental and experimental conditions afforded by microfluidic technologies presents unique opportunities that are particularly relevant to research and clinical applications requiring the controlled manipulation of blood. A critical bottleneck impeding the translation of existing Lab-on-Chip technology from laboratory bench to the clinic is the ability to reliably handle relatively small blood samples without negatively impacting blood composition or function. This review explores design considerations critical to the development of microfluidic systems intended for use with whole blood from an engineering perspective. Material hemocompatibility is briefly explored, encompassing common microfluidic device materials, as well as surface modification strategies intended to improve hemocompatibility. Operational hemocompatibility, including shear-induced effects, temperature dependence, and gas interactions are explored, microfluidic sample preparation methodologies are introduced, as well as current techniques for on-chip manipulation of the whole blood. Finally, methods of assessing hemocompatibility are briefly introduced, with an emphasis on primary hemostasis and platelet function.

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“…Fabrication of the microfluidic modules was accomplished utilising a combination of the 'membrane sandwich' approach 35 , and a novel PDMS Injection moulding technique, previously described by our group 28 Please do not adjust margins Please do not adjust margins plasma bonded to the interface slab; this assembled PDMS module can then be aligned over the sensor surface of the BiMW sensor and reversibly bonded. A thin (250-500 µm) PDMS cap can then be placed over the top of the module, this layer has cut-outs allowing access for pneumatic interface tubing, it is reversibly bonded and prevents evaporation of fluid from the on-chip reservoirs.…”

Section: Microfluidic Fabricationmentioning

confidence: 99%

“…We have recently begun to explore the possibility for complex integrated microfluidics and integrated optics 27 . We present here an automated fluid handling module designed for nanophotonic biosensors with complex valved microfluidics realised utilising a simple one-step injection moulding technique 28 , which can achieve complicated valved microfluidics which significantly reduce fabrication complexity and have the potential for scale-up mass manufacture. We demonstrate the utility of this microfluidic by integrating it with a highly sensitive Bimodal Waveguide photonic biosensor 4 , and demonstrate the functionality of the complete integrated system by performing repeated automated competitive immunoassays for the detection of an analyte of small molecular weight (the antibiotic tretacycline).…”

Section: Paper Introductionmentioning

confidence: 99%

An automated optofluidic biosensor platform combining interferometric sensors and injection moulded microfluidics

et al. 2017

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A primary limitation preventing practical implementation of photonic biosensors within point-of-care platforms is their integration with fluidic automation subsystems. For most diagnostic applications, photonic biosensors require complex fluid handling protocols; this is especially prominent in the case of competitive immunoassays, commonly used for detection of low-concentration, low-molecular weight biomarkers. For this reason, complex automated microfluidic systems are needed to realise the full point-of-care potential of photonic biosensors. To fulfil this requirement, we propose an on-chip valve-based microfluidic automation module, capable of automating such complex fluid handling. This module is realised through application of a PDMS injection moulding fabrication technique, recently described in our previous work, which enables practical fabrication of normally closed pneumatically actuated elastomeric valves. In this work, these valves are configured to achieve multiplexed reagent addressing for an on-chip diaphragm pump, providing the sample and reagent processing capabilities required for automation of cyclic competitive immunoassays. Application of this technique simplifies fabrication and introduces the potential for mass production, bringing point-of-care integration of complex automated microfluidics into the realm of practicality. This module is integrated with a highly sensitive, label-free bimodal waveguide photonic biosensor, and is demonstrated in the context of a proof-of-concept biosensing assay, detecting the low-molecular weight antibiotic tetracycline.

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Fabrication of complex PDMS microfluidic structures and embedded functional substrates by one-step injection moulding

Abstract: We report a novel injection moulding technique for fabrication of complex multi-layer microfluidic structures, allowing one-step robust integration of functional components with microfluidic channels and fabrication of elastomeric valves.

Cited by 32 publications

References 41 publications

Application of Microfluidic Chip Technology in Food Safety Sensing

Application of Microfluidic Chip Technology in Food Safety Sensing

A Review of Design Considerations for Hemocompatibility within Microfluidic Systems

An automated optofluidic biosensor platform combining interferometric sensors and injection moulded microfluidics

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