Fabrication of Cu Nanowires and its Effect on Proliferation of Mesenchymal Stem Cells

Abstract

Many studies have focused on the fabrication of Cu nanowires, because of their potential applications in diverse fields such as nanoelectronics and gene delivery. In this study, we successfully fabricated Cu nanowires by electrochemical deposition method. Cu nanowires was synthesized by electroplating from a 0.4M CuSO4 bath, and the current was 0.005A. The length of nanowires can be controlled via deposition time and current. After depositing for 60 minutes, the length of the nanowires is approximately 10 µm. Serum coated and non-coated nanowires with the concentration of 102, 103, 104 and 105 were added to 96-well cell culture plate in which mesenchymal stem cells were pre-seeded. After incubating for 3 days, cytotoxicity of nanowires was measured with MTT assay method. Results indicated that cytotoxicity increased as the increase of nanowires concentration. Serum coated nanowires had higher cell viability as compared with the non-coated group.

Cytotoxic effects of nickel nanowires in human fibroblasts

“…Cytotoxicity studies of NWs have shown a dependence on factors like material and size. A high concentration of NWs might produce very low toxicity and good biocompatibility in some cell lines such as HeLa cells [34] , 3T3 fibroblasts (CRL-1658) [11] and L929 mouse fibroblast [20] , while being highly toxic for other cell lines including mesenchymal stem cells [35] and human colorectal carcinoma cells (HCT 116) [28] . These results together with other contributions have made Ni NWs very interesting for biomedical applications.…”