Fabrication of Cultured Oral Gingiva by Tissue Engineering Techniques Without Materials of Animal Origin

Yamada, Kazuto; Yamaura, Junji; Katoh, Masakazu; Hata, Ken‐ichiro; Okuda, K; Yoshie, Hiromasa

doi:10.1902/jop.2006.050223

Cited by 29 publications

(20 citation statements)

References 29 publications

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“…In the last decades there have been great advances in this area, which led to its application in different medical fields, including periodontology. Some authors have been evaluating non-enzymatic methods of gingival fibroblasts isolation in vitro for the use in gingival augmentation (6,(10)(11)(12)(13)(14). Gingival fibroblast culture in different matrices has been studied and has shown promising results in soft tissues regeneration (12)(13)(14).…”

Section: Discussionmentioning

confidence: 99%

Morphological, Functional and Biochemical Characterization of Canine Gingival Fibroblasts

et al. 2013

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As dogs are good models for in vivo studies, it is interesting to evaluate the behavior of canine gingival fibroblasts (CGF) in vitro, so that these cells could be seeded on a matrix and later studied in vivo. The aim of this study was to perform a morphological, functional and biochemical analysis of CGF, comparing it with human gingival fibroblasts (HGF), as well as to evaluate the change of their characteristics over several passages. Using gingival fibroblasts from 3 dogs and 3 humans in the subculture (Sub), first (P1), third (P3), fifth (P5) and seventh (P7) passages, the following parameters were assessed: cell morphology, spreading, adhesion, viability and total protein content. The results showed no major differences between the passages in terms of morphology and spreading, and a tendency of greater adhesion and viability for HGF when compared with CGF. The total protein content was significantly higher for HGF. HGF exhibited greater functional and biochemical activity in vitro compared to CGF. Higher numbers at Sub were observed for both CGF and HGF in all evaluated parameters. The differences do not prevent the use of CGF for tissue engineering, but its use seems to be more appropriate in the subculture or first passage.

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Section: Discussionmentioning

confidence: 99%

Morphological, Functional and Biochemical Characterization of Canine Gingival Fibroblasts

et al. 2013

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“…One of the prospective sources comes from marine organisms (sea urchins, fish scale and skin, jellyfish, shark skin). Many studies indicated that bird feet, frog skin, sea urchin and shark skin collagen have a molecular structure different than domestic animals [10][11][12]. Their amino acid composition, peptide constitution, glycosaminoglycan content and thermal behavior are significantly different from land animals.…”

mentioning

confidence: 99%

An Estimation of the Biological Properties of Fish Collagen in an Experimental In Vitro Study

Kuzan¹,

Smulczyńska-Demel²,

Chwiłkowska³

et al. 2015

Adv Clin Exp Med

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Background. The principal sources of medical collagen are pork, calf skin and bone. There are now more studies on a much safer, alternative source of active collagen, mainly from aquatic life. Active collagen and its peptides FCP (fish collagen peptides) have already been extracted from the skin of salmon, cobia, hoki, tilapia, zebrafish, ling, shark, silver carp and also jellyfish. Objectives. The aim of the study is to evaluate the effect of fish collagen on human fibroblasts from gingiva. The cytotoxicity of the new formulation and induction of endogenous collagen was estimated by means of the collagen derived from fish skin. Material and Methods. Fish collagen was extracted from the skin of silver carp at 16 degrees Celsius. To compare the biocompatibility and endogenous collagen production Geistlich Bio-Gide ® membrane was ordered in Geistlich Biomaterials (Geistich AG, Wolhusen, Switzerland). The culture of human fibroblasts was performed acc. to Saczko et al. The fibroblasts were treated 96 hours with 1.0%, 0.5% and 0.1% experimental collagen formulation to induce endogenous collagen production. The Sircol collagen assay was done to measure amount of collagen. Cell viability was assessed by measuring mitochondrial activity in MTT assay after 24 h followed by 24 h of incubation with experimental collagen formulation. Qualitative analysis was performed by immunocytochemically staining of collagen type I and III. Results. Preparations of fish collagen are not cytotoxic at concentrations below 1%. Cells cultured in the presence of this product are characterized by a large number of endogenous collagen, which is comparable to the control. In case of porcine collagen membrane was noticed decreased to 83% production of endogenous collagen and reduction of cell viability to 69%. Conclusions. Our study showed that experimental fish collagen is an innovative product which may induce expression of endogenous collagen in fibroblasts (Adv Clin Exp Med 2015, 24, 3, 385-392).

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“…*0.01 \ p \ 0.05, **p \ 0.01; no asterisk means no significant difference (after applying for Bonferroni correction). Error bar means SD (N = 9) Cytotechnology (2015) 67:507-514 511 to Eagle's minimal essential medium: HEPES, L-glutamine, lipids, sodium bicarbonate, sodium selenite, gentamicin sulfate, non-essential amino acids, recombinant human insulin, recombinant human epidermal growth factor, dexamethasone, and synthetic polymer (Itoh and Hoshi 2005;Yamada et al 2006;Morimoto et al 2011;Takami et al 2008). It contains no animal-derived components and is thought to promote a normal fibroblast phenotype.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, Dulbecco's modified Eagle medium (DMEM) plus fetal bovine serum (FBS) is usually used for human fibroblast culture (Rittié and Fisher 2005). An autologous serum can be used in place of FBS (Itoh and Hoshi 2005;Yamada et al 2006;Nishi et al 2010;Morimoto et al 2011). Fibroblasts cultured with serum have a high proliferative capacity, similar to that of keloids; however, human fibroblasts in living dermis rarely proliferate and maintain homeostasis of the dermis through collagen production.…”

Section: Introductionmentioning

confidence: 99%

“…The manufacturers of HFDM-1 have demonstrated that short-term (9 day) culture of fibroblasts in serum-free HFDM-1 and long-term (20 day) culture of fibroblasts in HFDM-1 plus 0.5 % human serum allowed cells to proliferate as well as cells cultured in DMEM plus 10 % FBS (Itoh and Hoshi 2005). Additionally, several investigators have used HFDM-1 as a culture medium (Yamada et al 2006;Nishi et al 2010;Takami et al 2008). However, no studies have described the growth and biochemical characteristics of fibroblasts cultured in serum-free HFDM-1 in detail.…”

Section: Introductionmentioning

confidence: 99%

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Use of synthetic serum-free medium for culture of human dermal fibroblasts to establish an experimental system similar to living dermis

et al. 2014

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In this study, we sought to establish a defined experimental system for fibroblast growth similar to that of the living dermis. To this end, we evaluated the growth and biochemical characteristics of fibroblasts cultured with serum-free HFDM-1, a finely tuned synthetic medium for human fibroblast culture. Three culture conditions were used to grow fibroblasts obtained from primary culture: (1) culture with Dulbecco's modified Eagle medium (DMEM) plus 10 % fetal bovine serum (serum-supplemented DMEM), (2) culture with DMEM (serum-free DMEM), and (3) culture with HFDM-1 (HFDM-1), and fibroblast morphology, growth, collagen type I production, and lipid composition were analyzed. Fibroblasts grown in HFDM-1 maintained cell numbers at nearly 100 % from days 14 to 21 and produced more collagen type I than cells grown in serum-supplemented and serum-free DMEM. Arachidonic acid (20:4) and total polyunsaturated fatty acids were lower in cells grown in serum-free DMEM and HFDM-1 than in serum-supplemented DMEM. These results suggested that HFDM-1 recapitulated growth conditions in the dermis better than traditional, serum-supplemented DMEM. In addition, the controlled chemical composition of HFDM-1 eliminated a potential source of variability in cell culture conditions.

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Fabrication of Cultured Oral Gingiva by Tissue Engineering Techniques Without Materials of Animal Origin

Abstract: The new cultured gingival substitute containing no animal-derived materials produced good cell proliferation and VEGF release. The results suggested that the substitute may provide a new tool for the treatment of gingival recession.

Cited by 29 publications

References 29 publications

Morphological, Functional and Biochemical Characterization of Canine Gingival Fibroblasts

Morphological, Functional and Biochemical Characterization of Canine Gingival Fibroblasts

An Estimation of the Biological Properties of Fish Collagen in an Experimental In Vitro Study

Use of synthetic serum-free medium for culture of human dermal fibroblasts to establish an experimental system similar to living dermis

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